Invitrogen

CRTC3 Monoclonal Antibody (R.185.4)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

View all (13) CRTC3 antibodies

Datasheet

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Datasheet

Questions & Answers

Product Image Gallery

CRTC3 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hela (Lane 1), A431 (Lane 2) U2OS (Lane 3), HEK293 (Lane 4), Jurkat (Lane 5), COS-7 (Lane 6) and MCF-7 (Lane 7). The blot was probed with Anti-Torc3 Monoclonal Antibody (Product # MA5-14919, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 67 kDa band corresponding to Torc3 was observed in HeLa, U2OS and HEK293 when compared to other cell lines which are reported to have low levels of Torc3 expression.

CRTC3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Torc3 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-Torc3 Rabbit Monoclonal Antibody (Product # MA5-14919) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CRTC3 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-2 OS cells were transfected with Torc3 siRNA and reduction of signal was observed in Western Blot using Torc3 Monoclonal Antibody (R.185.4) (Product # MA5-14919). {KD}

CRTC3 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target in cell lines owing to their inherent genetic constitution. Differential basal expression of Torc3 was observed in HeLa, U2OS and HEK293 when compared to other cell lines which are reported to have low levels of Torc3 expression using product (Product # MA5-14919) in western blot. {RE}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1000

Immunocytochemistry (ICC/IF)

1:200

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

R.185.4

Immunogen

Synthetic peptide corresponding to the sequence of human TORC3 (CRTC3) protein

Conjugate

Unconjugated

Form

Liquid

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

RRID

AB_10979546

Product Specific Information

It is not recommended to aliquot this antibody.

Target Information

The protein encoded by this gene has similarity to a yeast protein. It seems to be part of a peripheral membrane protein complex localized on cis/medial Golgi cisternae where it may participate in tethering intra-Golgi transport vesicles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CREB regulated transcription coactivator 3; CREB-regulated transcription coactivator 3; TORC-3; transducer of CREB protein 3; transducer of regulated cAMP response element-binding protein (CREB) 3; Transducer of regulated cAMP response element-binding protein 3; transducer of regulated CREB protein 3

Gene Aliases: 2610312F20Rik; 6332415K15Rik; AI429792; BC011210; CRTC3; RGD1309666; TORC-3; TORC3

UniProt ID: (Human) Q6UUV7, (Mouse) Q91X84

Entrez Gene ID: (Human) 64784, (Mouse) 70461, (Rat) 365297

Molecular Function: CREB transcription factor basic leucine zipper transcription factor transcription factor