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Invitrogen
CRYAB Polyclonal Antibody
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FIGURE: 1 / 1
CRYAB Antibody (PA5-89366) in WB
Western blot analysis of CRYAB in extracts of various cell lines. Samples were incubated with CRYAB Polyclonal antibody (Product # PA5-89366) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-89366
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human CRYAB (NP_0018761)
Conjugate
Form
Liquid
Concentration
1.1 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR PPSFLRAPSW FDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVT AAPKK; Positive Samples: U-87MG, 293T, A-549, Mouse brain, Mouse heart, Mouse kidney, Mouse lung, Rat heart; Cellular Location: Cytoplasm, Nucleus
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha B-crystallin; Alpha(B)-crystallin; Alpha-crystallin B chain; CryAB antibody; crystallin, alpha 2; crystallin, alpha B; epididymis secretory protein Li 101; Heat shock protein beta-5; heat shock protein CryAB; Heat shock protein family B member 5; heat shock protein HspB5; heat-shock 20 kD like-protein; HspB5; P23; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component
Gene Aliases: AACRYA; CMD1II; Crya-2; CRYA2; CRYAB; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2; P23
UniProt ID: (Human) P02511, (Rat) P23928, (Mouse) P23927
Entrez Gene ID: (Human) 1410, (Rat) 25420, (Mouse) 12955
response to hypoxia
lens development in camera-type eye
protein folding
muscle contraction
tubulin complex assembly
muscle organ development
aging
negative regulation of gene expression
regulation of cell death
negative regulation of cell growth
microtubule polymerization or depolymerization
response to estradiol
negative regulation of intracellular transport
response to hydrogen peroxide
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
protein homooligomerization
stress-activated MAPK cascade
apoptotic process involved in morphogenesis
cellular response to gamma radiation
regulation of cellular response to heat
negative regulation of reactive oxygen species metabolic process
camera-type eye development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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