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Invitrogen
CRYAB Polyclonal Antibody
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FIGURE: 1 / 4
CRYAB Antibody (PA5-97626) in ICC/IF
Immunofluorescent analysis of CRYAB in U251 cells using a CRYAB polyclonal antibody (Product # PA5-97626) at a dilution of 1:66. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
Product Details
PA5-97626
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Alpha-crystallin B chain protein (1-175AA)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Lens proteins consist almost entirely of crystallins (about 95%). Crystallins are also found vertebrate skeletal muscle tissue. In the lens, their structural function is to assist in maintaining the proper refractive index of the lens. The mammalian lens contains 3 major classes of crystallins: alpha, beta, and gamma. Alpha-crystallin is the largest of the crystallins and is composed of 2 primary gene products-alpha-A and alpha-B. There are at least 5 different proteins comprising the beta-crystallins. The gamma-crystallins are monomeric, but there are at least 5 gamma crystallins identified in bovine and rat lens. Alpha-Crystallin comprises 40% of total lens protein composition. In addition to maintaining proper refractive index, it also functions in a chaperone like manner by preventing the formation of aggregates possibly leading to cataract formation. It is believed that the phosphorylated states of the alpha-crystallin occur in response to cellular stress and may serve a structural control function and play a role in protein maintenance. Alpha-B crystallin has been linked to Alexander's disease where it accumulates in brain cells of those afflicted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha B-crystallin; Alpha(B)-crystallin; Alpha-crystallin B chain; CryAB antibody; crystallin, alpha 2; epididymis secretory protein Li 101; heat shock 20 kDa protein-like protein; heat shock protein beta-5; Heat shock protein beta-5; heat shock protein CryAB; Heat shock protein family B member 5; heat shock protein HspB5; heat-shock 20 kD like-protein; HspB5; P23; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component; similar to human alpha B-crystallin Rosenthal fiber component encoded by GenBank Accession Number S45630; unnamed protein product
Gene Aliases: AACRYA; CMD1II; Crya-2; CRYA2; CRYAB; CTPP2; CTRCT16; HEL-S-101; HSPB5; MFM2; MFM2A; MFM2B; P23
UniProt ID: (Human) P02511, (Rat) P23928, (Mouse) P23927
Entrez Gene ID: (Human) 1410, (Rat) 25420, (Mouse) 12955
response to hypoxia
lens development in camera-type eye
protein folding
muscle contraction
tubulin complex assembly
muscle organ development
response to heat
negative regulation of metabolic process
negative regulation of gene expression
negative regulation of cell growth
microtubule polymerization or depolymerization
negative regulation of protein complex assembly
response to estradiol
negative regulation of intracellular transport
protein refolding
response to hydrogen peroxide
camera-type eye development
negative regulation of apoptotic process
regulation of programmed cell death
negative regulation of transcription, DNA-templated
protein stabilization
stress-activated MAPK cascade
apoptotic process involved in morphogenesis
cellular response to gamma radiation
negative regulation of amyloid fibril formation
negative regulation of reactive oxygen species metabolic process
regulation of cell death
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
protein homooligomerization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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