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Invitrogen
CSF1R Polyclonal Antibody
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FIGURE: 1 / 7
CSF1R Antibody (PA5-25974) in ICC/IF
Immunofluorescence analysis of CSF1R was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-CSF1R Polyclonal Antibody (Product # PA5-25974) at 1:200 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More
Product Details
PA5-25974
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Horse,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
CSF1R recombinant protein.
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: c-fms; CD115; CD115 antigen; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; FMS proto-oncogene; kinase CSFR; macrophage colony stimulating factor I receptor; Macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; OTTHUMP00000224093; Proto-oncogene c-Fms; proto-oncogene fms; transmembrane protein; unnamed protein product
Gene Aliases: AI323359; BANDDOS; C-FMS; CD115; CSF-1R; CSF1R; Csfmr; CSFR; Fim-2; FIM2; FMS; GPSC; HDLS; HDLS1; M-CSF-R; M-CSFR
UniProt ID: (Human) P07333, (Mouse) P09581
Entrez Gene ID: (Human) 1436, (Mouse) 12978
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
macrophage colony-stimulating factor receptor activity
protein binding
ATP binding
kinase activity
transferase activity
growth factor binding
protein phosphatase binding
cytokine binding
protein homodimerization activity
transferase activity, transferring phosphorus-containing groups
transmembrane signal receptor
positive regulation of protein phosphorylation
immune system process
myeloid leukocyte differentiation
response to ischemia
response to stress
inflammatory response
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
axon guidance
cell proliferation
positive regulation of cell proliferation
negative regulation of cell proliferation
regulation of cell shape
anatomical structure morphogenesis
positive regulation of macrophage chemotaxis
cell migration
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
olfactory bulb development
forebrain neuron differentiation
regulation of cell morphogenesis
hemopoiesis
monocyte differentiation
macrophage differentiation
osteoclast differentiation
positive regulation of cell migration
ruffle organization
positive regulation of chemokine production
regulation of actin cytoskeleton organization
cellular response to macrophage colony-stimulating factor stimulus
macrophage colony-stimulating factor signaling pathway
positive regulation of tyrosine phosphorylation of STAT protein
negative regulation of apoptotic process
regulation of MAPK cascade
positive regulation by host of viral process
innate immune response
regulation of bone resorption
cell-cell junction maintenance
protein autophosphorylation
animal organ development
positive regulation of protein kinase B signaling
mammary gland duct morphogenesis
positive regulation of protein tyrosine kinase activity
microglial cell proliferation
positive regulation of ERK1 and ERK2 cascade
cellular response to cytokine stimulus
positive regulation of macrophage proliferation
regulation of macrophage migration
positive regulation of cell motility
protein phosphorylation
phosphorylation
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of osteoclast differentiation
phosphatidylinositol metabolic process
phosphatidylinositol-mediated signaling
positive regulation of protein serine/threonine kinase activity
positive regulation of chemokine secretion
regulation of actin cytoskeleton reorganization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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