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Fibronectin Monoclonal Antibody (A17)
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FIGURE: 1 / 2
Fibronectin Antibody (CSI 005-17-02) in ICC/IF
BMC developmental biology 2007 -
Figure 2 Results of the immunofluorescent experiments for KRT18, FN1 and MYL6 . Confocal laser scanning images of in vitro produced bovine embryos labelled with primary mouse antibodies for KRT18, FN1 and MYL6 respectively in combination with FITC-labelled secondary goat-anti-mouse antibodies. The nuclei are stained with propidium iodide. Different stages of preimplantation embryo development were analysed ... View More
Product Details
CSI 005-17-02
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
A17
Immunogen
Lysed bovine corneal endothelial cells and extracellular matrix.
Conjugate
Form
Liquid
Concentration
1.04 mg/mL
Amount
416 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
CSI 005-17-02 is highly specific for fibronectin. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, elastin, collagen, laminin). CSI 005-17-02 cross-reacts with human and chicken fibronectin. Other species have not been tested.
CSI 005-17-02 has been successfully used in Western blottin, ELISA, Immunohistochemistry and Immunoprecipitation procedures. The antibody inhibits integrin-mediated cell adhesion to the cell binding domain of fibronectin. It can be used to probe fibronectin conformation. Strong reaction is seen in ELISA with thrombospondin directly coated onto the microtiter well.
The specificity of the CSI 005-17-02 epitope is located in the 120 kDa cell binding fragment.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; cumulus cell-specific fibronectin 1 transcript; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; embryo-specific fibronectin 1 transcript; epididymis secretory sperm binding protein; extracellular matrix protein; ferritin L subunit; ferritin L-chain; Fibronectin; fibronectin ED-A- region; fibronectin variable region; FN; lnc-ABCA12-8; Migration stimulating factor (MSF); migration-stimulating factor; unnamed protein product
Gene Aliases: CIG; ED-B; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF
UniProt ID: (Human) P02751, (Chicken) P11722
Entrez Gene ID: (Human) 2335, (Bovine) 280794, (Chicken) 396133
protease binding
receptor binding
integrin binding
extracellular matrix structural constituent
protein binding
collagen binding
heparin binding
peptidase activator activity
identical protein binding
proteoglycan binding
receptor agonist activity
disordered domain specific binding
extracellular matrix protein
extracellular matrix structural protein
angiogenesis
regulation of protein phosphorylation
acute-phase response
cell-substrate junction assembly
cell adhesion
cell-matrix adhesion
calcium-independent cell-matrix adhesion
integrin-mediated signaling pathway
nervous system development
heart development
positive regulation of cell proliferation
regulation of cell shape
response to wounding
positive regulation of gene expression
response to muscle activity
integrin activation
substrate adhesion-dependent cell spreading
endodermal cell differentiation
wound healing
endothelial cell migration
positive regulation of axon extension
positive regulation of fibroblast proliferation
interaction with symbiont
positive regulation of protein kinase B signaling
regulation of ERK1 and ERK2 cascade
negative regulation of transforming growth factor beta production
blood coagulation, fibrin clot formation
negative regulation of monocyte activation
neural crest cell migration involved in autonomic nervous system development
positive regulation of substrate-dependent cell migration, cell attachment to substrate
cell migration
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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