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Invitrogen
CSK Polyclonal Antibody
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FIGURE: 1 / 4
CSK Antibody (PA5-79076) in IHC (P)
Immunohistochemical analysis of Csk in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Csk antibody (Product # PA5-79076) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used... View More
Product Details
PA5-79076
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human CSK recombinant protein (Position: S2-G204).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: rat testis tissue, rat thymus tissue, mouse liver tissue, human Hela whole cell, human Jurkat whole cell, human A549 whole cell, human MCF-7 whole cell, mouse NIH/3T3 whole cell, mouse NEURO whole cell. IHC: human tonsil tissue, human tonsil tissue.
Target Information
CSK is a tyrosine kinase that has been shown to downregulate the activity of SRC oncoprotein through phosphorylation of its carboxy terminus. Since cell transformation by SRC is caused by various mechanisms that interfere with this phosphorylation, the CSK gene might function as an antioncogene. CSK plays an important role in the regulation of cell growth, differentiation, migration, and immune responses. CSK phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases including LCK, SRC, HCK, FYN, LYN, CSK, and YES1. CSK is expressed ubiquitously as a protein of approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts due to interaction with the lipid raft-associated protein PAG (Csk-binding protein).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-SRC kinase; MGC117393; p50CSK; protein-tyrosine kinase (CSK); Protein-tyrosine kinase CYL; Protein-tyrosine kinase MPK-2; Tyrosine-protein kinase CSK
Gene Aliases: AW212630; CSK; p50CSK
UniProt ID: (Human) P41240, (Rat) P32577, (Mouse) P41241
Entrez Gene ID: (Human) 1445, (Rat) 315707, (Mouse) 12988
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
receptor binding
protein binding
ATP binding
protein C-terminus binding
kinase activity
protein phosphatase binding
protein kinase A catalytic subunit binding
identical protein binding
metal ion binding
proline-rich region binding
morphogenesis of an epithelium
protein phosphorylation
transmembrane receptor protein tyrosine kinase signaling pathway
brain development
negative regulation of cell proliferation
negative regulation of low-density lipoprotein particle clearance
cell migration
negative regulation of interleukin-6 production
negative regulation of kinase activity
adherens junction organization
peptidyl-tyrosine autophosphorylation
negative regulation of Golgi to plasma membrane protein transport
positive regulation of MAP kinase activity
innate immune response
negative regulation of bone resorption
protein autophosphorylation
oligodendrocyte differentiation
negative regulation of phagocytosis
regulation of Fc receptor mediated stimulatory signaling pathway
negative regulation of ERK1 and ERK2 cascade
cellular response to peptide hormone stimulus
regulation of cytokine production
adaptive immune response
epidermal growth factor receptor signaling pathway
central nervous system development
cell differentiation
T cell costimulation
regulation of cell proliferation
T cell receptor signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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