Active Motif

CTCF Polyclonal Antibody

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Antibody Testing Data (4)

CTCF Antibody in ChIP-Sequencing (Chip-Seq)

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CTCF Antibody (61311) in Chip-Seq

CTCF antibody (rAb) tested by ChIP-Seq Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT High Sensitivity Kit (Cat. No. 53040) with MCF7-SER breast cancer cell line chromatin and 4 µg of CTCF antibody. ChIP DNA was sequenced on the Illumina NextSeq and 15.4 million sequence tags were mapped to identify CTCF binding sites on chromosome 12.

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CTCF Antibody in Immunocytochemistry (ICC/IF) — CTCF antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with CTCF antibody at a 1:2,000 dilution.

CTCF Antibody in Western Blot (WB) — CTCF pAb tested by Western blot. CTCF detection by Western blot. The analysis was performed using 20 µg HeLa nuclear cell extract and CTCF at a 1:500 dilution.

CTCF Antibody in ChIP Assay (ChIP) — CTCF antibody (pAb) tested by ChIP analysis. Chromatin IP performed using the ChIP-IT® Express Kit (Catalog No. 53008) and HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) using 8 µl of CTCF antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the SCN4A gene (site A or site B). Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG (arbitrarily assigned a value of 1) using the ddCT method.

Product Details

61311

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (IHC)

1:1,000

Immunocytochemistry (ICC/IF)

1:2,000

ChIP assay (ChIP)

4 µL

ChIP-sequencing (ChIP-Seq)

2-8 µL

View 3 publications 3 publications

in situ PLA (PLA)

View 1 publication 1 publication

Gel Shift (GS)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 10 publications 10 publications

Product Specifications

Species Reactivity

Human

Published species

Chicken, Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

This CTCF antibody was raised against a peptide within the N-terminal region of human CTCF.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.5, with 30% glycerol

Contains

0.035% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2614975

Target Information

Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms' tumors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DNA (de)methylation in embryonic stem cells controls CTCF-dependent chromatin boundaries.

Genome research

Wiehle L,Thorn GJ,Raddatz G,Clarkson CT,Rippe K,Lyko F,Breiling A,Teif VB

Research topic: DNA methylation, Gene Expression

Wed May 01 00:00:00 EDT 2019

Trim33 is required for appropriate development of pre-cardiogenic mesoderm.

Developmental biology

Rajderkar S,Mann JM,Panaretos C,Yumoto K,Li HD,Mishina Y,Ralston B,Kaartinen V

Research topic: Cardiology, Developmental Biology

Sat Jun 15 00:00:00 EDT 2019

The anti-cancer drugs curaxins target spatial genome organization.

Nature communications

Kantidze OL,Luzhin AV,Nizovtseva EV,Safina A,Valieva ME,Golov AK,Velichko AK,Lyubitelev AV,Feofanov AV,Gurova KV,Studitsky VM,Razin SV

Research topic: Chromatin Structure

Fri Mar 29 00:00:00 EDT 2019

Linking aberrant chromatin features in chronic lymphocytic leukemia to transcription factor networks.

Molecular systems biology

Mallm JP,Iskar M,Ishaque N,Klett LC,Kugler SJ,Muino JM,Teif VB,Poos AM,Großmann S,Erdel F,Tavernari D,Koser SD,Schumacher S,Brors B,König R,Remondini D,Vingron M,Stilgenbauer S,Lichter P,Zapatka M,Mertens D,Rippe K

Research topic: Chronic lymphocytic leukemia (CLL)

Wed May 22 00:00:00 EDT 2019

lncRNA KHPS1 Activates a Poised Enhancer by Triplex-Dependent Recruitment of Epigenomic Regulators.

Cell reports

Blank-Giwojna A,Postepska-Igielska A,Grummt I

Research topic: Transcription

Tue Mar 12 00:00:00 EDT 2019

lncRNA KHPS1 Activates a Poised Enhancer by Triplex-Dependent Recruitment of Epigenomic Regulators.

Cell reports

Blank-Giwojna A,Postepska-Igielska A,Grummt I

Research topic: Transcription

Tue Mar 12 00:00:00 EDT 2019

Batf Pioneers the Reorganization of Chromatin in Developing Effector T Cells via Ets1-Dependent Recruitment of Ctcf.

Cell reports

Pham D,Moseley CE,Gao M,Savic D,Winstead CJ,Sun M,Kee BL,Myers RM,Weaver CT,Hatton RD

Research topic: Immunology, Transcription

Tue Oct 29 00:00:00 EDT 2019

Identification of Therapeutic Targets in Rhabdomyosarcoma through Integrated Genomic, Epigenomic, and Proteomic Analyses.

Cancer cell

Stewart E,McEvoy J,Wang H,Chen X,Honnell V,Ocarz M,Gordon B,Dapper J,Blankenship K,Yang Y,Li Y,Shaw TI,Cho JH,Wang X,Xu B,Gupta P,Fan Y,Liu Y,Rusch M,Griffiths L,Jeon J,Freeman BB,Clay MR,Pappo A,Easton J,Shurtleff S,Shelat A,Zhou X,Boggs K,Mulder H,Yergeau D,Bahrami A,Mardis ER,Wilson RK,Zhang J,Peng J,Downing JR,Dyer MA

Research topic: Skeletal Muscle - Rhabdomyosarcoma

Mon Sep 10 00:00:00 EDT 2018

PLoS biology

Yakushiji-Kaminatsui N,Lopez-Delisle L,Bolt CC,Andrey G,Beccari L,Duboule D

Research topic: Embryogenesis

Thu Nov 01 00:00:00 EDT 2018

PLoS biology

Yakushiji-Kaminatsui N,Lopez-Delisle L,Bolt CC,Andrey G,Beccari L,Duboule D

Research topic: Embryogenesis

Thu Nov 01 00:00:00 EDT 2018

SIRT1 mediates obesity- and nutrient-dependent perturbation of pubertal timing by epigenetically controlling Kiss1 expression.

Nature communications

Vazquez MJ,Toro CA,Castellano JM,Ruiz-Pino F,Roa J,Beiroa D,Heras V,Velasco I,Dieguez C,Pinilla L,Gaytan F,Nogueiras R,Bosch MA,Rønnekleiv OK,Lomniczi A,Ojeda SR,Tena-Sempere M

Research topic: Prostate Cancer

Wed Oct 10 00:00:00 EDT 2018

CXXC1 is not essential for normal DNA double-strand break formation and meiotic recombination in mouse.

PLoS genetics

Tian H,Billings T,Petkov PM

Research topic: Virology (HPV)

Mon Oct 01 00:00:00 EDT 2018

Pan-cancer analysis of somatic copy-number alterations implicates IRS4 and IGF2 in enhancer hijacking.

Nature genetics

Weischenfeldt J,Dubash T,Drainas AP,Mardin BR,Chen Y,Stütz AM,Waszak SM,Bosco G,Halvorsen AR,Raeder B,Efthymiopoulos T,Erkek S,Siegl C,Brenner H,Brustugun OT,Dieter SM,Northcott PA,Petersen I,Pfister SM,Schneider M,Solberg SK,Thunissen E,Weichert W,Zichner T,Thomas R,Peifer M,Helland A,Ball CR,Jechlinger M,Sotillo R,Glimm H,Korbel JO

Research topic: Cancer

Sun Jan 01 00:00:00 EST 2017

The Dynamic Epigenetic Landscape of the Retina During Development, Reprogramming, and Tumorigenesis.

Neuron

Aldiri I,Xu B,Wang L,Chen X,Hiler D,Griffiths L,Valentine M,Shirinifard A,Thiagarajan S,Sablauer A,Barabas ME,Zhang J,Johnson D,Frase S,Zhou X,Easton J,Zhang J,Mardis ER,Wilson RK,Downing JR,Dyer MA

Research topic: Eyes

Wed May 03 00:00:00 EDT 2017

Genome-Wide Binding of Posterior HOXA/D Transcription Factors Reveals Subgrouping and Association with CTCF.

PLoS genetics

Jerković I,Ibrahim DM,Andrey G,Haas S,Hansen P,Janetzki C,González Navarrete I,Robinson PN,Hecht J,Mundlos S

Research topic: Chromstin Structure

Sun Jan 01 00:00:00 EST 2017

The Human GATA1 Gene Retains a 5' Insulator That Maintains Chromosomal Architecture and GATA1 Expression Levels in Splenic Erythroblasts.

Molecular and cellular biology

Moriguchi T,Yu L,Takai J,Hayashi M,Satoh H,Suzuki M,Ohneda K,Yamamoto M

Research topic: Erythropoiesis

Fri May 01 00:00:00 EDT 2015

The Human GATA1 Gene Retains a 5' Insulator That Maintains Chromosomal Architecture and GATA1 Expression Levels in Splenic Erythroblasts.

Molecular and cellular biology

Moriguchi T,Yu L,Takai J,Hayashi M,Satoh H,Suzuki M,Ohneda K,Yamamoto M

Research topic: Erythropoiesis

Fri May 01 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: 11 zinc finger transcriptional repressor; 11-zinc finger protein; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein); CTCFL paralog; OTTHUMP00000197610; Transcriptional repressor CTCF

Gene Aliases: CTCF; MRD21

UniProt ID: (Human) P49711

Entrez Gene ID: (Human) 10664

Molecular Function: C2H2 zinc finger transcription factor

Function(s)

negative regulation of transcription from RNA polymerase II promoter DNA methylation regulation of gene expression by genetic imprinting transcription from RNA polymerase II promoter chromosome segregation maintenance of DNA methylation positive regulation of gene expression covalent chromatin modification nucleosome positioning regulation of histone methylation regulation of histone acetylation regulation of gene expression, epigenetic regulation of molecular function, epigenetic negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of centromeric sister chromatid cohesion

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding zinc ion binding chromatin insulator sequence binding sequence-specific DNA binding transcription regulatory region DNA binding

CTCF Polyclonal Antibody

CTCF Antibody (61311) in Chip-Seq

Product Details

61311

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

ChIP assay (ChIP)

ChIP-sequencing (ChIP-Seq)

in situ PLA (PLA)

Gel Shift (GS)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Purification

Storage buffer

Contains

Storage conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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