Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (IHC) |
1:1,000 | - |
Immunocytochemistry (ICC/IF) |
1:2,000 | - |
ChIP assay (ChIP) |
4 µL | - |
in situ PLA (PLA) |
- | View 1 publication 1 publication |
Gel Shift (GS) |
- | View 1 publication 1 publication |
ChIP-sequencing (ChIP-Seq) |
2-8 µL | View 3 publications 3 publications |
Miscellaneous PubMed (Misc) |
- | View 10 publications 10 publications |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Chicken, Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
This CTCF antibody was raised against a peptide within the N-terminal region of human CTCF. |
Conjugate |
Unconjugated |
Form |
Liquid |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.5, with 30% glycerol |
Contains |
0.035% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2614975 |
Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms' tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 11 zinc finger transcriptional repressor; 11-zinc finger protein; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein); CTCFL paralog; OTTHUMP00000197610; Transcriptional repressor CTCF
Gene Aliases: CTCF; MRD21
UniProt ID: (Human) P49711
Entrez Gene ID: (Human) 10664
Molecular Function:
C2H2 zinc finger transcription factor
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