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Invitrogen
CTCF Polyclonal Antibody
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FIGURE: 1 / 10
CTCF Antibody (PA5-88115) in ICC/IF
Immunofluorescence analysis of CTCF was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CTCF Polyclonal Antibody (Product # PA5-88115) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (... View More
Product Details
PA5-88115
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human CTCF (NP_0065561)
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
50 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MEGDAVEAIV EESETFIKGK ERKTYQRRRE GGQEEDACHL PQNQTDGGEV VQDVNSSVQM VMMEQLDPTL LQMKTEVMEG TVAPEAEAAV DDTQIITLQV VNMEEQPINI GELQLVQVPV PVTVPVATTS VEELQGAYEN EVSKEGLAES EPMICHTLPL PEGFQVVKVG ANGEVETLEQ GELPPQEDPS WQKDPDYQPP AKKTKKTKKS KLRYTEEGKD VDVSVYDFEE EQQEGLLSEV NAEKVVGNMK PPKPTKIKKK; Positive Samples: 293T, Jurkat; Cellular Location: Chromosome, Nucleus, centromere, nucleoplasm
Target Information
Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively. It is a multifunctional protein involved in the transactivation of APPB gene, silencing of c-myc gene, and insulation of human beta-globin and DMI myotonic dystrophy locus and imprinting control of IGF2 and H19 regions. CTCF has also been shown to regulate the transcription of Pax6 and IRAK2 promoter thereby opening a therapeutic avenue. Mutations in this gene have been associated with invasive breast cancers, prostate cancers and Wilms' tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 11 zinc finger transcriptional repressor; 11-zinc finger protein; CCCTC-binding factor; CCCTC-binding factor (zinc finger protein); CTCFL paralog; OTTHUMP00000197610; Transcriptional repressor CTCF; unnamed protein product
Gene Aliases: AW108038; CFAP108; CTCF; FAP108; MRD21
UniProt ID: (Human) P49711, (Rat) Q9R1D1, (Mouse) Q61164
Entrez Gene ID: (Human) 10664, (Rat) 83726, (Mouse) 13018
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcription cofactor binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
zinc ion binding
chromatin insulator sequence binding
sequence-specific DNA binding
metal ion binding
chromatin loop anchoring activity
sequence-specific double-stranded DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription regulatory region DNA binding
nucleic acid binding
C2H2 zinc finger transcription factor
negative regulation of transcription from RNA polymerase II promoter
in utero embryonic development
chromatin organization
methylation-dependent chromatin silencing
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
mitochondrion organization
chromosome segregation
heart development
negative regulation of cell proliferation
gene expression
positive regulation of macromolecule biosynthetic process
positive regulation of gene expression
negative regulation of gene expression
protein localization to chromosome
regulation of gene expression, epigenetic
hypermethylation of CpG island
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
cardiac muscle cell differentiation
cardiac muscle cell development
regulation of centromeric sister chromatid cohesion
protein localization to chromosome, centromeric region
genetic imprinting
chromatin looping
DNA methylation
regulation of gene expression by genetic imprinting
transcription, DNA-templated
transcription from RNA polymerase II promoter
maintenance of DNA methylation
chromatin modification
nucleosome positioning
regulation of histone methylation
regulation of histone acetylation
regulation of molecular function, epigenetic
dosage compensation by inactivation of X chromosome
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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