Proteintech

CTHRC1 Polyclonal Antibody

View all (13) CTHRC1 antibodies

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Questions & Answers

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CTHRC1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed A375 cells using 16534-1-AP (CTHRC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CTHRC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 16534-1-AP (CTHRC1 antibody) at dilution of 1:50 (under 40x lens).

CTHRC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 16534-1-AP (CTHRC1 antibody) at dilution of 1:50 (under 10x lens).

CTHRC1 Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CTHRC1 Antibody in Western Blot (WB) — Mouse lung tissue were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CTHRC1 Antibody in Western Blot (WB) — Rat lung tissue were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CTHRC1 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CTHRC1 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

CTHRC1 Antibody in Western Blot (WB) — A375 cells were subjected to SDS PAGE followed by western blot with 16534-1-AP (CTHRC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

CTHRC1 Antibody in Immunoprecipitation (IP) — IP result of anti-CTHRC1 (IP:16534-1-AP, 3ug; Detection:16534-1-AP 1:500) with A375 cells lysate 3600ug.

Product Details

16534-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 4 publications 4 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CTHRC1 Fusion Protein Ag9812 (1-243 aa encoded by BC014245) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.21 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MRPQGPAAS PQRLRGLLLL LLLQLPAPSS ASEIPKGKQK AQLRQREVVD LYNGMCLQGP AGVPGRDGSP GANGIPGTPG IPGRDGFKGE KGECLRESFE ESWTPNYKQC SWSSLNYGID LGKIAECTFT KMRSNSALRV LFSGSLRLKC RNACCQRWYF TFNGAECSGP LPIEAIIYLD QGSPEMNSTI NIHRTSSVEG LCEGIGAGLV DVAIWVGTCS DYPKGDASTG WNSVSRIIIE ELPK (1-243 aa encoded by BC014245)

Target Information

The Collagen triple helix repeat-containing protein (CTHRC1) is a glycosylated, secreted protein that is transiently expressed in the arterial wall in response to injury where it is thought to contribute to vascular remodeling by inhibiting collagen expression and deposition and promoting cell migration. CTHRC1 is highly expressed multiple human cancers, including pancreatic cancer where it plays a significant role in the progression and metastasis of the disease by regulating migration and adhesion of tumors cells. CTHRC1 overexpression can also be used as an independent prognostic marker in gastric cancer. Mutations at this locus have been associated with Barrett esophagus and esophageal adenocarcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CTHRC1 mediates IL‑1β‑induced apoptosis in chondrocytes via JNK1/2 signaling.

International journal of molecular medicine

Zhang Q,Yin ZS,Zhang FW,Cao K,Sun HY

Sun Apr 01 00:00:00 EDT 2018

Collagen triple helix repeat containing 1 (CTHRC1) activates Integrin β3/FAK signaling and promotes metastasis in ovarian cancer.

Journal of ovarian research

Guo B,Yan H,Li L,Yin K,Ji F,Zhang S

Wed Oct 11 00:00:00 EDT 2017

Collagen triple helix repeat containing 1 (CTHRC1) activates Integrin β3/FAK signaling and promotes metastasis in ovarian cancer.

Journal of ovarian research

Guo B,Yan H,Li L,Yin K,Ji F,Zhang S

Wed Oct 11 00:00:00 EDT 2017

Lentivirus-Mediated Knockdown of CTHRC1 Inhibits Osteosarcoma Cell Proliferation and Migration.

Cancer biotherapy & radiopharmaceuticals

Sang W,Zhu L,Ma J,Lu H,Wang C

Fri Apr 01 00:00:00 EDT 2016

SP1-mediated microRNA-520d-5p suppresses tumor growth and metastasis in colorectal cancer by targeting CTHRC1.

American journal of cancer research

Yan L,Yu J,Tan F,Ye GT,Shen ZY,Liu H,Zhang Y,Wang JF,Zhu XJ,Li GX

Tue Jun 23 00:00:00 EDT 2015

MiR-9 inhibits Schwann cell migration by targeting Cthrc1 following sciatic nerve injury.

Journal of cell science

Zhou S,Gao R,Hu W,Qian T,Wang N,Ding G,Ding F,Yu B,Gu X

Sat Mar 01 00:00:00 EST 2014

MiR-9 inhibits Schwann cell migration by targeting Cthrc1 following sciatic nerve injury.

Journal of cell science

Zhou S,Gao R,Hu W,Qian T,Wang N,Ding G,Ding F,Yu B,Gu X

Sat Mar 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: Collagen triple helix repeat-containing protein 1

Gene Aliases: 1110014B07Rik; CTHRC1; UNQ762/PRO1550

UniProt ID: (Human) Q96CG8, (Mouse) Q9D1D6, (Rat) Q8CG08

Entrez Gene ID: (Human) 115908, (Mouse) 68588, (Rat) 282836

Molecular Function: oxygenase

cell migration positive regulation of protein binding positive regulation of osteoblast proliferation ossification involved in bone remodeling positive regulation of osteoblast differentiation Wnt signaling pathway, planar cell polarity pathway inner ear receptor stereocilium organization negative regulation of canonical Wnt signaling pathway cochlea morphogenesis establishment of planar polarity involved in neural tube closure

Process(es)

frizzled binding Wnt-protein binding protein binding