Proteintech

CUL4A Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

CUL4A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using CUL4A antibody 14851-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

CUL4A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 14851-1-AP ( CUL4A-Specific Antibody) at dilution of 1:50 (under 40x lens).

CUL4A Antibody in Western Blot (WB) — Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14851-1-AP (CUL4A-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CUL4A Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug CUL4A-Specific antibody (14851-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

Product Details

14851-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 20 publications 20 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody is specifically against CUL4A,but not CUL4B. CUL4A has 2 isoforms with MW 88 kDa (759 aa) and 77 kDa (659 aa), and 14851-1-AP recognizes both.

Target Information

CUL4A (Cullin 4A) is the ubiquitin ligase component of a multimeric complex involved in the degradation of DNA damage-response proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Genetic Modulation of BET1L Confers Colorectal Cancer Susceptibility by Reducing miRNA Binding and m6A Modification.

Cancer research

Li S,Du M,Xu K,Ben S,Zhu T,Guo M,Xin J,Zhu L,Gu D,Zhang Z,Wang M

Wed Jul 05 00:00:00 EDT 2023

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy.

Signal transduction and targeted therapy

Meng Y,Qiu L,Zeng X,Hu X,Zhang Y,Wan X,Mao X,Wu J,Xu Y,Xiong Q,Chen Z,Zhang B,Han J

Fri Dec 09 00:00:00 EST 2022

Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy.

Signal transduction and targeted therapy

Meng Y,Qiu L,Zeng X,Hu X,Zhang Y,Wan X,Mao X,Wu J,Xu Y,Xiong Q,Chen Z,Zhang B,Han J

Fri Dec 09 00:00:00 EST 2022

AMPK induces degradation of the transcriptional repressor PROX1 impairing branched amino acid metabolism and tumourigenesis.

Nature communications

Wang Y,Luo M,Wang F,Tong Y,Li L,Shu Y,Qiao K,Zhang L,Yan G,Liu J,Ji H,Xie Y,Zhang Y,Gao WQ,Liu Y

Thu Nov 24 00:00:00 EST 2022

MOF negatively regulates estrogen receptor α signaling via CUL4B-mediated protein degradation in breast cancer.

Frontiers in oncology

Zhang X,Yang Y,Li D,Wu Z,Liu H,Zhao Z,Zhu H,Xie F,Li X

Tue Oct 11 00:00:00 EDT 2022

Evaluation of HZX-960, a novel DCN1-UBC12 interaction inhibitor, as a potential antifibrotic compound for liver fibrosis.

Biochemistry and cell biology = Biochimie et biologie cellulaire

Zhou W,Dong G,Gao G,He Z,Xu J,Aziz S,Ma L,Zhao W

Mon Aug 01 00:00:00 EDT 2022

Discovery of Potent and Selective 2-(Benzylthio)pyrimidine-based DCN1-UBC12 Inhibitors for Anticardiac Fibrotic Effects.

Journal of medicinal chemistry

He ZX,An Q,Wei B,Zhou WJ,Wei BF,Gong YP,Zhang X,Gao G,Dong GJ,Huo JL,Zhang XH,Yang FF,Liu HM,Ma LY,Zhao W

Thu Jan 13 00:00:00 EST 2022

Cardioprotective effect of MLN4924 on ameliorating autophagic flux impairment in myocardial ischemia-reperfusion injury by Sirt1.

Redox biology

Zhang J,Cui J,Zhao F,Yang L,Xu X,Shi Y,Wei B

Fri Oct 01 00:00:00 EDT 2021

The Mediator subunit MED20 organizes the early adipogenic complex to promote development of adipose tissues and diet-induced obesity.

Cell reports

Tang WS,Weng L,Wang X,Liu CQ,Hu GS,Yin ST,Tao Y,Hong NN,Guo H,Liu W,Wang HR,Zhao TJ

Tue Jul 06 00:00:00 EDT 2021

DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity.

Cell reports

Wang X,Wang HY,Hu GS,Tang WS,Weng L,Zhang Y,Guo H,Yao SS,Liu SY,Zhang GL,Han Y,Liu M,Zhang XD,Cen X,Shen HF,Xiao N,Liu CQ,Wang HR,Huang J,Liu W,Li P,Zhao TJ

Tue Jun 22 00:00:00 EDT 2021

Negative regulation of AMPK signaling by high glucose via E3 ubiquitin ligase MG53.

Molecular cell

Jiang P,Ren L,Zhi L,Yu Z,Lv F,Xu F,Peng W,Bai X,Cheng K,Quan L,Zhang X,Wang X,Zhang Y,Yang D,Hu X,Xiao RP

Thu Feb 04 00:00:00 EST 2021

The E3 ubiquitin ligase Cul4b promotes CD4+ T cell expansion by aiding the repair of damaged DNA.

PLoS biology

Dar AA,Sawada K,Dybas JM,Moser EK,Lewis EL,Park E,Fazelinia H,Spruce LA,Ding H,Seeholzer SH,Oliver PM

Mon Feb 01 00:00:00 EST 2021

Functional impacts of the ubiquitin-proteasome system on DNA damage recognition in global genome nucleotide excision repair.

Scientific reports

Sakai W,Yuasa-Sunagawa M,Kusakabe M,Kishimoto A,Matsui T,Kaneko Y,Akagi JI,Huyghe N,Ikura M,Ikura T,Hanaoka F,Yokoi M,Sugasawa K

Thu Nov 12 00:00:00 EST 2020

Effect of CUL4A on the metastatic potential of lung adenocarcinoma to the bone.

Oncology reports

Cao PP,Chen WJ,Pang HL,Shen WW,Xue P,Duan L,Xu YX,Liu LL,Zhang HL

Sat Feb 01 00:00:00 EST 2020

Cul4a as a New Interaction Protein of PARP1 Inhibits Oxidative Stress-Induced H9c2 Cell Apoptosis.

Oxidative medicine and cellular longevity

Ye N,Zhang N,Zhang Y,Qian H,Wu B,Sun Y

Thu Jan 16 00:00:00 EST 2020

Potent 5-Cyano-6-phenyl-pyrimidin-Based Derivatives Targeting DCN1-UBE2M Interaction.

Journal of medicinal chemistry

Zhou W,Ma L,Ding L,Guo Q,He Z,Yang J,Qiao H,Li L,Yang J,Yu S,Zhao L,Wang S,Liu HM,Suo Z,Zhao W

Thu Jun 13 00:00:00 EDT 2019

Cul4 E3 ubiquitin ligase regulates ovarian cancer drug resistance by targeting the antiapoptotic protein BIRC3.

Cell death & disease

Hu X,Meng Y,Xu L,Qiu L,Wei M,Su D,Qi X,Wang Z,Yang S,Liu C,Han J

Mon Feb 04 00:00:00 EST 2019

Cul4-Ddb1 ubiquitin ligases facilitate DNA replication-coupled sister chromatid cohesion through regulation of cohesin acetyltransferase Esco2.

PLoS genetics

Sun H,Zhang J,Xin S,Jiang M,Zhang J,Li Z,Cao Q,Lou H

Fri Feb 01 00:00:00 EST 2019

Amyloid Precursor Protein (APP) May Act as a Substrate and a Recognition Unit for CRL4CRBN and Stub1 E3 Ligases Facilitating Ubiquitination of Proteins Involved in Presynaptic Functions and Neurodegeneration.

The Journal of biological chemistry

Del Prete D,Rice RC,Rajadhyaksha AM,D'Adamio L

Fri Aug 12 00:00:00 EDT 2016

The Journal of biological chemistry

Del Prete D,Rice RC,Rajadhyaksha AM,D'Adamio L

Fri Aug 12 00:00:00 EDT 2016

AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.

Developmental cell

Antonioli M,Albiero F,Nazio F,Vescovo T,Perdomo AB,Corazzari M,Marsella C,Piselli P,Gretzmeier C,Dengjel J,Cecconi F,Piacentini M,Fimia GM

Mon Dec 22 00:00:00 EST 2014

AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.

Developmental cell

Antonioli M,Albiero F,Nazio F,Vescovo T,Perdomo AB,Corazzari M,Marsella C,Piselli P,Gretzmeier C,Dengjel J,Cecconi F,Piacentini M,Fimia GM

Mon Dec 22 00:00:00 EST 2014

CSN6 drives carcinogenesis by positively regulating Myc stability.

Nature communications

Chen J,Shin JH,Zhao R,Phan L,Wang H,Xue Y,Post SM,Ho Choi H,Chen JS,Wang E,Zhou Z,Tseng C,Gully C,Velazquez-Torres G,Fuentes-Mattei E,Yeung G,Qiao Y,Chou PC,Su CH,Hsieh YC,Hsu SL,Ohshiro K,Shaikenov T,Wang H,Yeung SC,Lee MH