Proteintech

CUL4B Polyclonal Antibody

View all (31) Cullin 4B antibodies

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CUL4B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 20882-1-AP (CUL4B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CUL4B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 20882-1-AP ( CUL4B Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CUL4B Antibody in Western Blot (WB) — Human testis tissue were subjected to SDS PAGE followed by western blot with 20882-1-AP (CUL4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

CUL4B Antibody in Immunoprecipitation (IP) — IP result of anti-CUL4B (IP:20882-1-AP, 4ug; Detection:20882-1-AP 1:300) with mouse brain tissue lysate 4000ug.

Product Details

20882-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CUL4B Fusion Protein Ag15024 (1-195 aa encoded by BC036216) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Proteintech's CUL4B antibody 20882-1-AP can specifically recognize CUL4B.

Immunogen sequence: MMSQSSGSG DGNDDEATTS KDGGFSSPSP SAAAAAQEVR SATDGNTSTT PPTSAKKRKL NSSSSSSSNS SNEREDFDST SSSSSTPPLQ PRDSASPSTS SFCLGVSVAA SSHVPIQKKL RFEDTLEFVG FDAKMAEESS SSSSSSSPTA ATSQQQQLKN KSILISSVAS VHHANGLAKS STTVSSFANS KPGSAK (1-195 aa encoded by B C036216)

Target Information

Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition subunit. CUL4B may act within the complex as a scaffold protein, contributing to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Plays a role as part of the E3 ubiquitin-protein ligase complex in polyubiquitination of CDT1, histone H2A, histone H3 and histone H4 in response to radiation-induced DNA damage. Targeted to UV damaged chromatin by DDB2 and may be important for DNA repair and DNA replication. Required for ubiquitination of cyclin E, and consequently, normal G1 cell cycle progression. Regulates the mammalian target-of-rapamycin (mTOR) pathway involved in control of cell growth, size and metabolism. Specific CUL4B regulation of the mTORC1-mediated pathway is dependent upon 26S proteasome function and requires interaction between CUL4B and MLST8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.