Immunofluorescent analysis of CUTC showing staining in the cytoplasm and nucleus of H1299 cells. H1299 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a CUTC polyclonal antibody (Product # PA5-31586) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 206 of Human CUTC|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31586 targets CUTC in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-31586 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 206 of Human CUTC.
Members of the CUT family of copper transporters are associated with copper homeostasis and are involved in the uptake, storage, delivery, and efflux of copper (Gupta et al., 1995 [PubMed 7635807]; Li et al., 2005 [PubMed 16182249]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.