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Immunofluorescent analysis of HeLa cells using a CUX1 polyclonal antibody (Product # PA5-25788). HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a CUX1 polyclonal antibody (Product # PA5-25788) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 1347-1374 amino acids from the C-terminal region of human CUX1|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is a member of the homeodomain family of DNA binding proteins. It may regulate gene expression, morphogenesis, and differentiation and it may also play a role in the cell cycle progession. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CASP; CCAAT displacement protein; CDP; CDP/Cut; CDP1; Clox; COY1; cut homolog; cut-like homeobox 1; CUTL1; CUX; Cux/CDP; golgi integral membrane protein 6; GOLIM6; homeobox protein cux-1; Nbla10317; p200; protein CASP; putative protein product of Nbla10317
CASP; CDP; CDP/Cut; CDP1; Clox; COY1; CUTL1; CUX; Cux/CDP; CUX1; GOLIM6; Nbla10317; p100; p110; p200; p75