Invitrogen

CX3CL1 Polyclonal Antibody

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CX3CL1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of CX3CL1 in HeLa cells. Samples were incubated in CX3CL1 polyclonal antibody (Product # PA5-23062). CX3CL (green). Nuclei (Blue) are counterstained using Hoechst 33258..

CX3CL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CX3CL1 in mouse brain. Samples were incubated in CX3CL1 polyclonal antibody (Product # PA5-23062).

CX3CL1 Antibody in Western Blot (WB) — Western blot was performed using Anti-CX3CL1 Polyclonal Antibody (Product # PA5-23062) and a 85 kDa band corresponding to soluble cleaved form of CX3CL1 was observed across tested cell line along with uncharacterized (*) bands at ~180 kDa and 60 kDa. Conditioned media (20 µg lysate) of HUVEC (Lane 1), HUVEC treated with TNF-alpha, 10 ng/ml for 12 hrs (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

CX3CL1 Antibody in Immunohistochemistry (IHC) — Figure 4 Protein expression of CX3CL1 and CX3CR1 evaluated in hepatoblastomas and hepatoblastoma lung metastasis by immunohistochemistry assay. (A-D) Show CX3CL1 data, and (A1-D1) , CX3CR1. (A) TCH361, CX3CL1 strong positivity of infiltrated lymphocytes (indicated by arrow 1) in necrotic regions of the tumor, and (A1) , CX3CR1 strong positivity of infiltrated lymphocytes (indicated by arrow 2) in necrotic regions of the tumor; (B,B1) TCH327, positivity in tumor cells (indicated by arrows 3 and 5) and infiltrated lymphocytes negative (indicated by arrows 4 and 6) for both proteins. (C) TCH321, positivity in the osteoblast component and strong positivity in the fetal type (indicated by arrow 7); infiltrated lymphocytes are negative (indicated by arrow 8); (C1) positivity in tumor cells and lymphocytes negative; (D,D1) TCH360, lung metastasis showing positivity in tumor cells (indicated by arrows 9 and 11), and no expression in infiltrated lymphocytes (indicated by arrows 10 and 12), for both proteins.

Product Details

PA5-23062

Applications	Tested Dilution	Publications
Western Blot (WB)	Assay-Dependent	-
Immunohistochemistry (Paraffin) (IHC (P))	1:250	-
Immunohistochemistry (Frozen) (IHC (F))	Assay-Dependent	-
Immunocytochemistry (ICC/IF)	1:100	-
Immunohistochemistry (IHC)	-	View 3 publications 3 publications

Product Specifications
Species Reactivity	Gerbil, Human, Mouse
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthetic peptide made to an internal region of the human CX3CL protein (within residues 20-150). View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.15 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 30% glycerol
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11152552

Target Information

Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-a in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Insights Into the Somatic Mutation Burden of Hepatoblastomas From Brazilian Patients.

Frontiers in oncology

Aguiar TFM,Rivas MP,Costa S,Maschietto M,Rodrigues T,Sobral de Barros J,Barbosa AC,Valieris R,Fernandes GR,Bertola DR,Cypriano M,Caminada de Toledo SR,Major A,Tojal I,Apezzato MLP,Carraro DM,Rosenberg C,Lima da Costa CM,Cunha IW,Sarabia SF,Terrada DL,Krepischi ACV

PA5-23062 was used in Immunohistochemistry to investigate somatic mutations in a cohort of 10 hepatoblastomas, including a congenital case.

Mon Sep 28 00:00:00 EDT 2020

Insights Into the Somatic Mutation Burden of Hepatoblastomas From Brazilian Patients.

Frontiers in oncology

PA5-23062 was used in Immunohistochemistry to investigate somatic mutations in a cohort of 10 hepatoblastomas, including a congenital case.

Mon Sep 28 00:00:00 EDT 2020

Augmented concentrations of CX3CL1 are associated with interstitial lung disease in systemic sclerosis.

PloS one

Hoffmann-Vold AM,Weigt SS,Palchevskiy V,Volkmann E,Saggar R,Li N,Midtvedt Ø,Lund MB,Garen T,Fishbein MC,Ardehali A,Ross DJ,Ueland T,Aukrust P,Lynch JP,Elashoff RM,Molberg Ø,Belperio JA

PA5-23062 was used in Immunohistochemistry to explore Fractalkine CX3CL1 in systemic sclerosis related progressive interstitial lung disease and pulmonary hypertension in two different but complementary sources of biomaterial.

Thu Apr 11 00:00:00 EDT 2019

Augmented concentrations of CX3CL1 are associated with interstitial lung disease in systemic sclerosis.

PloS one

Hoffmann-Vold AM,Weigt SS,Palchevskiy V,Volkmann E,Saggar R,Li N,Midtvedt Ø,Lund MB,Garen T,Fishbein MC,Ardehali A,Ross DJ,Ueland T,Aukrust P,Lynch JP,Elashoff RM,Molberg Ø,Belperio JA

Thu Apr 11 00:00:00 EDT 2019

CX₃CR1-CX₃CL1-dependent cell-to-cell Japanese encephalitis virus transmission by human microglial cells.

Scientific reports

Lannes N,Garcia-Nicolàs O,Démoulins T,Summerfield A,Filgueira L

PA5-23062 was used in Immunohistochemistry to suggest that human microglia may be a source of infection for neuronal populations and sustain Japanese encephalitis virus (JEV) brain pathogenesis in long-term infection.

Mon Mar 18 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: C-X3-C motif chemokine 1; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; FK; Fractalkine; Neurotactin; Processed fractalkine; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D, 1; Small-inducible cytokine D1

Gene Aliases: A-152E5.2; AB030188; ABCD-3; AI848747; C3Xkine; CX3C; CX3CL1; CXC3; CXC3C; D8Bwg0439e; FKN; fractalkine; neurotactin; NTN; NTT; SCYD1

UniProt ID: (Human) P78423, (Mouse) O35188

Entrez Gene ID: (Human) 6376, (Mouse) 20312

Molecular Function: cytokine intercellular signal molecule