Invitrogen

CXCL1 Polyclonal Antibody

Product Image Gallery

CXCL1 Antibody — Figure 7 Role of fisetin in regulating the CXCLs signaling pathway in mice with PRI-induced systemic lupus erythematosus. (A) Western blot analysis was used to determine the expression levels of CXCL-1, CXCL-2, CCL-3 and CXCR-2 in the spleen tissue samples obtained from mice subjected to various treatments. Representative images are provided. (B) Quantification of CXCL-1, CXCL-2, CCL-3 and CXCR-2 protein levels from the western blots. Values are expressed as the mean +- standard error of the mean (n=10 in each group). *** P<0.001 vs. the Con group; + P<0.05, ++ P<0.01 and +++ P<0.001 vs. the PRI group. Con, control; PRI, pristine; CXC, chemokine (C-X-C) motif; CXCL, CXC ligand; CXCR, CXC receptor; CCL-3, chemokine (C-C motif) ligand 3; F25/50/100, treatment with 25/50/100 mg/kg fisetin.

CXCL1 Antibody in Western Blot (WB) — Western blot analysis of CXCL1 in supernatant of KC-transfected cells. Samples were probed with a CXCL1 polyclonal antibody (Product # PA1-29220).

Product Details

Tested Applications

Dilution

Immunoprecipitation (IP)

1:300-1:800

Western Blot (WB)

1:2000-1:5000

Published Applications

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Not Applicable

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment - amino acids 1-72 of rat KC

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_1957060

Product Specific Information

PA1-29220 detects CXCL1 from rat and mouse samples.

PA1-29220 has been successfully used in immunoprecipitation and Western blot applications. It is not suitable for use in immunohistochemistry applications.

The PA1-29220 immunogen is recombinant fragment - amino acids 1-72 of rat KC.

Store at 4°C for short term storage. For long term storage aliquot and store at -20°C or below. Avoid freeze/thaw cycles.

Target Information

KC, a homolog of human and hamster gro/MGSA, is a 72 amino acid CXC chemokine originally cloned from rat macrophages and lung tissue. It is the mediator for recruitment and activation of neutrophils in rat lung inflammation models. Expression of KC can be upregulated by LPS and IL-1b stimulation. IFN-gamma blocks LPS induced expression of KC.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha-chemokine; C-X-C motif chemokine 1; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CINC-1; Cytokine-induced neutrophil chemoattractant 1; Fsp; gro; Gro1; GRO1 oncogene; Growth-regulated alpha protein; Hematopoietic synergistic factor; HSF; KC; KC(5-72); KC-T; KC/GR)-alpha; KC/GRO-alpha; Mgsa; N51; Platelet-derived growth factor-inducible protein KC; Scyb1; Secretory protein N51

Gene Aliases: CINC-1; Cinc1; Cxcl1; Fsp; Gro; Gro1; KC; Mgsa; N51; Scyb1

UniProt ID: (Rat) P14095, (Mouse) P12850

Entrez Gene ID: (Rat) 81503, (Mouse) 14825

Molecular Function: chemokine cytokine signaling molecule