Invitrogen

CXCL1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

4 References

View all (30) CXCL1 (GRO alpha) antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

FIGURE: 1 / 5

CXCL1 Antibody (PA1-29220) in WB

Rat KC detected by Western Blot in the supernatant of KC-transfected cells using CXCL1 Polyclonal Antibody (Product # PA1-29220).

Product Image Gallery

CXCL1 Antibody — Figure 8 Fisetin reduces inflammation and expression of CXCLs in LPS-induced RAW 264.7 cells. RAW 264.7 cells were pre-treated with LPS (100 ng/ml) for 2 h, followed by treatment with fisetin (15 or 30 u M) for another 24 h. (A) Flow cytometric analysis was used to quantify CD3-expressing cells. (B) CD3 expression levels were measured using western blot analysis. (C) Reverse transcription-quantitative polymerase chain reaction analysis of pro-inflammatory cytokines as indicated. (D) CXCL-1, CXCL-2, CCL-3 and CXCR-2 expression levels were assessed using western blot analysis. Values are expressed as the mean +- standard error of the mean (n=8 in each group). *** P<0.001 vs. the Con group; + P<0.05, ++ P<0.01 and +++ P<0.001 vs. the LPS group. Con, control; LPS, lipopolysaccharide; CXC, chemokine (C-X-C) motif; CXCL, CXC ligand; CXCR, CXC receptor; CCL-3, chemokine (C-C motif) ligand 3; F15/30, treatment with 15/30 u m fisetin. {TM}

Product Details

PA1-29220

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:5,000

View 4 publications 4 publications

Immunoprecipitation (IP)

1:300-1:800

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment - amino acids 1-72 of rat KC

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_1957060

Product Specific Information

PA1-29220 detects CXCL1 from rat and mouse samples.

Target Information

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The inflammation-resolution promoting molecule resolvin-D1 prevents atrial proarrhythmic remodelling in experimental right heart disease.

Cardiovascular research

Hiram R,Xiong F,Naud P,Xiao J,Sirois M,Tanguay JF,Tardif JC,Nattel S

PA1-29220 was used in Western Blot to evaluate resolvin-therapy effects on atrial arrhythmogenic remodelling in experimental right heart disease.

Wed Jun 16 00:00:00 EDT 2021

Nlrp12 Mediates Adverse Neutrophil Recruitment during Influenza Virus Infection.

Journal of immunology (Baltimore, Md. : 1950)

Hornick EE,Banoth B,Miller AM,Zacharias ZR,Jain N,Wilson ME,Gibson-Corley KN,Legge KL,Bishop GA,Sutterwala FS,Cassel SL

PA1-29220 was used in Western Blotting to determine the role of Nlrp12 in modulating neutrophilic responses during lethal influenza A virus infection.

Thu Feb 01 00:00:00 EST 2018

Fisetin inhibits pristine-induced systemic lupus erythematosus in a murine model through CXCLs regulation.

International journal of molecular medicine

Xu SP,Li YS

Published figure using CXCL1 polyclonal antibody (Product # PA1-29220) in Western Blot

Sat Dec 01 00:00:00 EST 2018

Identifying the functional contribution of the defatty-acylase activity of SIRT6.

Nature chemical biology

Zhang X,Khan S,Jiang H,Antonyak MA,Chen X,Spiegelman NA,Shrimp JH,Cerione RA,Lin H

Published figure using CXCL1 polyclonal antibody (Product # PA1-29220) in Western Blot

Mon Aug 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CINC-1; CXC; CXC motif chemokine; CXCL; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; gro; GRO a; GRO α; GRO1 oncogene; GROalpha; Growth-regulated alpha protein; GROα; Hematopoietic synergistic factor; HSF; KC(5-72); KC-T; KC/GR)-alpha; KC/GRO-alpha; NAP3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51

Gene Aliases: CINC-1; Cinc1; Cxcl1; Fsp; Gro; Gro1; KC; Mgsa; N51; Scyb1

UniProt ID: (Rat) P14095, (Mouse) P12850

Entrez Gene ID: (Rat) 81503, (Mouse) 14825

Molecular Function: chemokine