Invitrogen

CXCL1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

3 Published Figures

3 References

View all (24) CXCL1 (GRO alpha) antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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CXCL1 Antibody in Western Blot (WB) — Western blot analysis of CXCL1 in supernatant of KC-transfected cells. Samples were probed with a CXCL1 polyclonal antibody (Product # PA1-29220).

CXCL1 Antibody — Figure 8 Fisetin reduces inflammation and expression of CXCLs in LPS-induced RAW 264.7 cells. RAW 264.7 cells were pre-treated with LPS (100 ng/ml) for 2 h, followed by treatment with fisetin (15 or 30 u M) for another 24 h. (A) Flow cytometric analysis was used to quantify CD3-expressing cells. (B) CD3 expression levels were measured using western blot analysis. (C) Reverse transcription-quantitative polymerase chain reaction analysis of pro-inflammatory cytokines as indicated. (D) CXCL-1, CXCL-2, CCL-3 and CXCR-2 expression levels were assessed using western blot analysis. Values are expressed as the mean +- standard error of the mean (n=8 in each group). *** P<0.001 vs. the Con group; + P<0.05, ++ P<0.01 and +++ P<0.001 vs. the LPS group. Con, control; LPS, lipopolysaccharide; CXC, chemokine (C-X-C) motif; CXCL, CXC ligand; CXCR, CXC receptor; CCL-3, chemokine (C-C motif) ligand 3; F15/30, treatment with 15/30 u m fisetin. {TM}

Product Details

PA1-29220

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:5,000	View 3 publications 3 publications
Immunoprecipitation (IP)	1:300-1:800	-

Product Specifications
Species Reactivity	Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment - amino acids 1-72 of rat KC View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.2
Contains	0.1% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_1957060

Product Specific Information

PA1-29220 detects CXCL1 from rat and mouse samples.

Target Information

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fisetin inhibits pristine-induced systemic lupus erythematosus in a murine model through CXCLs regulation.

International journal of molecular medicine

Xu SP,Li YS

Published figure using CXCL1 polyclonal antibody (Product # PA1-29220) in Western Blot

Sat Dec 01 00:00:00 EST 2018

Nlrp12 Mediates Adverse Neutrophil Recruitment during Influenza Virus Infection.

Journal of immunology (Baltimore, Md. : 1950)

Hornick EE,Banoth B,Miller AM,Zacharias ZR,Jain N,Wilson ME,Gibson-Corley KN,Legge KL,Bishop GA,Sutterwala FS,Cassel SL

PA1-29220 was used in Western Blotting to determine the role of Nlrp12 in modulating neutrophilic responses during lethal influenza A virus infection.

Thu Feb 01 00:00:00 EST 2018

Identifying the functional contribution of the defatty-acylase activity of SIRT6.

Nature chemical biology

Zhang X,Khan S,Jiang H,Antonyak MA,Chen X,Spiegelman NA,Shrimp JH,Cerione RA,Lin H

Published figure using CXCL1 polyclonal antibody (Product # PA1-29220) in Western Blot

Mon Aug 01 00:00:00 EDT 2016

Bioinformatics

Protein Aliases: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CINC-1; CXC; CXC motif chemokine; CXCL; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; gro; GRO a; GRO α; GRO1 oncogene; GROalpha; Growth-regulated alpha protein; GROα; Hematopoietic synergistic factor; HSF; KC(5-72); KC-T; KC/GR)-alpha; KC/GRO-alpha; NAP3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51

Gene Aliases: CINC-1; Cinc1; Cxcl1; Fsp; Gro; Gro1; KC; Mgsa; N51; Scyb1

UniProt ID: (Rat) P14095, (Mouse) P12850

Entrez Gene ID: (Rat) 81503, (Mouse) 14825

Molecular Function: chemokine