Invitrogen

CXCL1 Polyclonal Antibody

Product Image Gallery

CXCL1 Antibody in Western Blot (WB) — Western blot analysis of CXCL1 in supernatant of KC-transfected cells. Samples were probed with a CXCL1 polyclonal antibody (Product # PA1-29220).

CXCL1 Antibody — Figure 7 Role of fisetin in regulating the CXCLs signaling pathway in mice with PRI-induced systemic lupus erythematosus. (A) Western blot analysis was used to determine the expression levels of CXCL-1, CXCL-2, CCL-3 and CXCR-2 in the spleen tissue samples obtained from mice subjected to various treatments. Representative images are provided. (B) Quantification of CXCL-1, CXCL-2, CCL-3 and CXCR-2 protein levels from the western blots. Values are expressed as the mean +- standard error of the mean (n=10 in each group). *** P<0.001 vs. the Con group; + P<0.05, ++ P<0.01 and +++ P<0.001 vs. the PRI group. Con, control; PRI, pristine; CXC, chemokine (C-X-C) motif; CXCL, CXC ligand; CXCR, CXC receptor; CCL-3, chemokine (C-C motif) ligand 3; F25/50/100, treatment with 25/50/100 mg/kg fisetin.

Product Details

Applications

Tested Dilution

Publications

Immunoprecipitation (IP)

1:300-1:800

Western Blot (WB)

1:2000-1:5000

View 3 publications 3 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment - amino acids 1-72 of rat KC

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_1957060

Product Specific Information

PA1-29220 detects CXCL1 from rat and mouse samples.

Target Information

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CINC-1; CXC; CXC motif chemokine; CXCL; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; gro; GRO a; GRO α; GRO1 oncogene; GROalpha; Growth-regulated alpha protein; GROα; Hematopoietic synergistic factor; HSF; KC(5-72); KC-T; KC/GR)-alpha; KC/GRO-alpha; NAP3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51

Gene Aliases: CINC-1; Cinc1; Cxcl1; Fsp; Gro; Gro1; KC; Mgsa; N51; Scyb1

UniProt ID: (Rat) P14095, (Mouse) P12850

Entrez Gene ID: (Rat) 81503, (Mouse) 14825

Molecular Function: chemokine cytokine signaling molecule