Invitrogen

CXCL1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

3 Published Figures

5 References

View all (30) CXCL1 (GRO alpha) antibodies

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Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

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CXCL1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Growth-regulated alpha protein was performed using 70% confluent log phase A-375 cells treated with 10 ng/mL TNF alpha for 24 hours and untreated A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CXCL1 Polyclonal Antibody (Product # PA5-86508) at 1:100 dilution in 0.1% BSA, incubated at 4°C overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green) in A-375 treated cells. Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization in TNF alpha treated A-375 cells. Panel e represents untreated A-375 cells that show no expression of CXCL1. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CXCL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CXCL1 in paraffin-embedded human tonsil carcinoma tissue. Sample was incubated with polyclonal CXCL1 antibody (Product # PA5-86508) using a 1:50 dilution.

CXCL1 Antibody in Western Blot (WB) — Western blot was performed using Anti-CXCL1 Polyclonal Antibody (Product # PA5-86508) and a 9 kDa band corresponding to soluble cleaved form of CXCL1 was observed across cell lines tested. Conditioned media (20 µg lysate) of Caki-1, positive model (Lane 1) and HT-29, negative model (Lane 2) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556).

CXCL1 Antibody in Immunohistochemistry (IHC) — Figure 6 The keratinocyte-target lipid-polymer nanohybrids suppress IMQ-induced cytokine and chemokine expression, as well as neutrophil infiltration in psoriasis-like skin. IHC analysis represents (A) IL-17, (B) CXCL1, (C) CXCL2, (D) Ki67 + , (E) Ly6G, and (F) MPO in psoriasis-like skin. Scale bars, 100 um.

CXCL1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-375 cells were transfected with CXCL1 siRNA followed by TNF alpha treatment and decrease in signal intensity was observed in ICC application using Anti-CXCL1 Polyclonal Antibody (Product # PA5-86508).

Product Details

PA5-86508

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, corresponding to amino acids 41-90 of Human GROalpha.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2803283

Target Information

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

LncRNA MEG3 ameliorates NiO nanoparticles-induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway.

Environmental toxicology

Yang M,Chang X,Gao Q,Gong X,Zheng J,Liu H,Li K,Zhan H,Wang X,Li S,Sun X,Feng S,Sun Y

PA5-86508 was used in Western Blotting to explore the roles of long noncoding RNA maternally expressed gene 3 (lncRNA MEG3) and p38 mitogen activated protein kinases (p38 MAPK) pathway in pulmonary inflammatory injury induced by NiO NPs.

Sun May 01 00:00:00 EDT 2022

CXCL1 Regulated by miR-302e Is Involved in Cell Viability and Motility of Colorectal Cancer via Inhibiting JAK-STAT Signaling Pathway.

Frontiers in oncology

Chen B,Song L,Nie X,Lin F,Yu Z,Kong W,Qi X,Wang W

PA5-86508 was used in Immunohistochemistry (Paraffin) to make a systemic description for the CXCL1-dependent regulatory mechanism in colorectal cancer.

Sat Apr 23 00:00:00 EDT 2022

CXCL1 Regulated by miR-302e Is Involved in Cell Viability and Motility of Colorectal Cancer via Inhibiting JAK-STAT Signaling Pathway.

Frontiers in oncology

Chen B,Song L,Nie X,Lin F,Yu Z,Kong W,Qi X,Wang W

PA5-86508 was used in Immunohistochemistry (Paraffin) to make a systemic description for the CXCL1-dependent regulatory mechanism in colorectal cancer.

Sat Apr 23 00:00:00 EDT 2022

Pharmacological perturbation of CXCL1 signaling alleviates neuropathogenesis in a model of HEVA71 infection.

Nature communications

Gunaseelan S,Ariffin MZ,Khanna S,Ooi MH,Perera D,Chu JJH,Chua JJE

PA5-86508 was used in Immunohistochemistry to highlight the CXCL1-CXCR2 signaling pathway as a potential target against HFMD neuropathogenesis.

Wed Feb 16 00:00:00 EST 2022

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation.

Theranostics

Lin ZC,Hwang TL,Huang TH,Tahara K,Trousil J,Fang JY

PA5-86508 was used in Immunohistochemistry to develop actively targeted nanocarriers loaded with the phosphodiesterase 4 inhibitor AN2728.

Mon Aug 16 00:00:00 EDT 2021

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation.

Theranostics

Lin ZC,Hwang TL,Huang TH,Tahara K,Trousil J,Fang JY

PA5-86508 was used in Immunohistochemistry to develop actively targeted nanocarriers loaded with the phosphodiesterase 4 inhibitor AN2728.

Mon Aug 16 00:00:00 EDT 2021

Involvement of CXCL1/CXCR2 During Microglia Activation Following Inflammation-Sensitized Hypoxic-Ischemic Brain Injury in Neonatal Rats.

Frontiers in neurology

Serdar M,Kempe K,Herrmann R,Picard D,Remke M,Herz J,Bendix I,Felderhoff-Müser U,Sabir H

PA5-86508 was used in Western Blot to indicate that the CXCL1/CXCR2 pathway may be involved during pro-inflammatory microglia activation following inflammation-sensitized hypoxic-ischemic brain injury in neonatal rats.

Sat Oct 31 00:00:00 EDT 2020

Involvement of CXCL1/CXCR2 During Microglia Activation Following Inflammation-Sensitized Hypoxic-Ischemic Brain Injury in Neonatal Rats.

Frontiers in neurology

Serdar M,Kempe K,Herrmann R,Picard D,Remke M,Herz J,Bendix I,Felderhoff-Müser U,Sabir H

Sat Oct 31 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CINC-1; CXC; CXC motif chemokine; CXCL; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; fibroblast secretory protein; gro; GRO a; GRO α; GRO-alpha(1-73); GRO-alpha(4-73); GRO-alpha(5-73); GRO-alpha(6-73); GRO1 oncogene; GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROalpha; Growth-regulated alpha protein; GROα; Hematopoietic synergistic factor; HSF; KC(5-72); KC-T; KC/GR)-alpha; KC/GRO-alpha; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; NAP-3; NAP3; Neutrophil-activating protein 3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51

Gene Aliases: CINC-1; Cinc1; CXCL1; FSP; GRO; GRO1; GROA; KC; MGSA; MGSA-a; N51; NAP-3; SCYB1

UniProt ID: (Human) P09341, (Rat) P14095, (Mouse) P12850

Entrez Gene ID: (Human) 2919, (Rat) 81503, (Mouse) 14825

Molecular Function: chemokine