Invitrogen

CXCL1 Polyclonal Antibody

CXCL1 Antibody in Immunocytochemistry (ICC/IF)

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CXCL1 Antibody (PA5-86508) in ICC/IF

Immunofluorescence analysis of Growth-regulated alpha protein was performed using 70% confluent log phase A-375 cells treated with 10 ng/mL TNF alpha for 24 hours and untreated A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CXCL1 Polyclonal Antibody (Product # PA5-86508) at 1:100 dilution in 0.1% BSA, incubated at 4°C overnight and then labeled with Donkey anti-Rabbit IgG (H+L... View More

CXCL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CXCL1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CXCL1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of Growth-regulated alpha protein was observed in Caki-1 (positive model) and HT-29 (negative model) using Anti-CXCL1 Polyclonal Antibody (Product # PA5-86508) in Western Blot. {RE}

Product Details

PA5-86508

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, corresponding to amino acids 41-90 of Human GROalpha.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2803283

Product Specific Information

Purity is > 95% by SDS-PAGE.

Target Information

CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

E3 ligase TRIM28 promotes anti-PD-1 resistance in non-small cell lung cancer by enhancing the recruitment of myeloid-derived suppressor cells.

Journal of experimental & clinical cancer research : CR

Liang M,Sun Z,Chen X,Wang L,Wang H,Qin L,Zhao W,Geng B

Published figure using CXCL1 polyclonal antibody (Product # PA5-86508) in Immunohistochemistry

Sat Oct 21 00:00:00 UTC 2023

Pharmacological perturbation of CXCL1 signaling alleviates neuropathogenesis in a model of HEVA71 infection.

Nature communications

Gunaseelan S,Ariffin MZ,Khanna S,Ooi MH,Perera D,Chu JJH,Chua JJE

PA5-86508 was used in Immunohistochemistry to highlight the CXCL1-CXCR2 signaling pathway as a potential target against HFMD neuropathogenesis.

Wed Feb 16 00:00:00 UTC 2022

Associations of C-X-C motif chemokine ligands 1/2/8/13/14 with clinicopathological features and survival profile in patients with colorectal cancer.

Oncology letters

Luo X,Tai J,Zhao Y,Zhao P,Sun D,Wang L

PA5-86508 was used in Immunohistochemistry to assess the correlation of C-X-C motif chemokine ligand (CXCL)1, CXCL2, CXCL8, CXCL13 and CXCL14 with clinicopathological features and survival profile in patients with colorectal cancer (CRC).

Sat Oct 01 00:00:00 UTC 2022

LncRNA MEG3 ameliorates NiO nanoparticles-induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway.

Environmental toxicology

Yang M,Chang X,Gao Q,Gong X,Zheng J,Liu H,Li K,Zhan H,Wang X,Li S,Sun X,Feng S,Sun Y

PA5-86508 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to explore the roles of long noncoding RNA maternally expressed gene 3 (lncRNA MEG3) and p38 mitogen activated protein kinases (p38 MAPK) pathway in pulmonary inflammatory injury induced by NiO NPs.

Sun May 01 00:00:00 UTC 2022

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation.

Theranostics

Lin ZC,Hwang TL,Huang TH,Tahara K,Trousil J,Fang JY

Published figure using CXCL1 polyclonal antibody (Product # PA5-86508) in Immunohistochemistry

Mon Aug 16 00:00:00 UTC 2021

Monovalent antibody-conjugated lipid-polymer nanohybrids for active targeting to desmoglein 3 of keratinocytes to attenuate psoriasiform inflammation.

Theranostics

Lin ZC,Hwang TL,Huang TH,Tahara K,Trousil J,Fang JY

Published figure using CXCL1 polyclonal antibody (Product # PA5-86508) in Immunohistochemistry

Mon Aug 16 00:00:00 UTC 2021

CXCL1 Regulated by miR-302e Is Involved in Cell Viability and Motility of Colorectal Cancer via Inhibiting JAK-STAT Signaling Pathway.

Frontiers in oncology

Chen B,Song L,Nie X,Lin F,Yu Z,Kong W,Qi X,Wang W

Published figure using CXCL1 polyclonal antibody (Product # PA5-86508) in Immunohistochemistry (Paraffin)

Sat Apr 23 00:00:00 UTC 2022

CXCL1 Regulated by miR-302e Is Involved in Cell Viability and Motility of Colorectal Cancer via Inhibiting JAK-STAT Signaling Pathway.

Frontiers in oncology

Chen B,Song L,Nie X,Lin F,Yu Z,Kong W,Qi X,Wang W

Published figure using CXCL1 polyclonal antibody (Product # PA5-86508) in Immunohistochemistry (Paraffin)

Sat Apr 23 00:00:00 UTC 2022

Involvement of CXCL1/CXCR2 During Microglia Activation Following Inflammation-Sensitized Hypoxic-Ischemic Brain Injury in Neonatal Rats.

Frontiers in neurology

Serdar M,Kempe K,Herrmann R,Picard D,Remke M,Herz J,Bendix I,Felderhoff-Müser U,Sabir H

PA5-86508 was used in Western Blot to indicate that the CXCL1/CXCR2 pathway may be involved during pro-inflammatory microglia activation following inflammation-sensitized hypoxic-ischemic brain injury in neonatal rats.

Sat Oct 31 00:00:00 UTC 2020

Involvement of CXCL1/CXCR2 During Microglia Activation Following Inflammation-Sensitized Hypoxic-Ischemic Brain Injury in Neonatal Rats.

Frontiers in neurology

Serdar M,Kempe K,Herrmann R,Picard D,Remke M,Herz J,Bendix I,Felderhoff-Müser U,Sabir H

Sat Oct 31 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CINC-1; CXC; CXC motif chemokine; CXCL; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; fibroblast secretory protein; gro; GRO a; GRO α; GRO-alpha(1-73); GRO1 oncogene; GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROalpha; Growth-regulated alpha protein; GROα; KC/GR)-alpha; KC/GRO-alpha; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; NAP-3; NAP3; Neutrophil-activating protein 3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51

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Gene Aliases: CINC-1; Cinc1; CXCL1; FSP; GRO; GRO1; GROA; KC; MGSA; MGSA-a; N51; NAP-3; SCYB1

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UniProt ID: (Human) P09341, (Rat) P14095, (Mouse) P12850

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Entrez Gene ID: (Human) 2919, (Rat) 81503, (Mouse) 14825

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Function(s)

receptor binding chemokine activity enzyme activator activity growth factor activity CXCR chemokine receptor binding cytokine activity chemokine

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CXCL1 Polyclonal Antibody

CXCL1 Antibody (PA5-86508) in ICC/IF

Product Details

PA5-86508

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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