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Invitrogen
CXCL1 Polyclonal Antibody
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FIGURE: 1 / 11
CXCL1 Antibody (PA5-86508) in ICC/IF
Immunofluorescence analysis of Growth-regulated alpha protein was performed using 70% confluent log phase A-375 cells treated with 10 ng/mL TNF alpha for 24 hours and untreated A-375 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CXCL1 Polyclonal Antibody (Product # PA5-86508) at 1:100 dilution in 0.1% BSA, incubated at 4°C overnight and then labeled with Donkey anti-Rabbit IgG (H+L... View More
Product Details
PA5-86508
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide, corresponding to amino acids 41-90 of Human GROalpha.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Purity is > 95% by SDS-PAGE.
Target Information
CXCL1, CXCL2, and CXCL3, also known respectively as GRO alpha, GRO beta (MIP-2 alpha) and GRO gamma (MIP-2 beta), are members of the CXC subfamily of chemokines. Mature CXCL1/2/3 proteins bind with high affinity to the IL-8 receptor type B and are potent neutrophil attractants and activators. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: alpha-chemokine; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 1; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha); CINC-1; CXC; CXC motif chemokine; CXCL; CXCL-1; Cytokine-induced neutrophil chemoattractant 1; fibroblast secretory protein; gro; GRO a; GRO α; GRO-alpha(1-73); GRO1 oncogene; GRO1 oncogene (melanoma growth stimulating activity, alpha); GRO1 oncogene (melanoma growth-stimulating activity); GROalpha; Growth-regulated alpha protein; GROα; KC/GR)-alpha; KC/GRO-alpha; melanoma growth stimulating activity, alpha; Melanoma growth stimulatory activity; melanoma growth stimulatory activity alpha; MGSA; MGSA alpha; NAP-3; NAP3; Neutrophil-activating protein 3; Platelet-derived growth factor-inducible protein KC; Secretory protein N51
Gene Aliases: CINC-1; Cinc1; CXCL1; FSP; GRO; GRO1; GROA; KC; MGSA; MGSA-a; N51; NAP-3; SCYB1
UniProt ID: (Human) P09341, (Rat) P14095, (Mouse) P12850
Entrez Gene ID: (Human) 2919, (Rat) 81503, (Mouse) 14825
chemotaxis
inflammatory response
immune response
signal transduction
G-protein coupled receptor signaling pathway
nervous system development
cell proliferation
negative regulation of cell proliferation
actin cytoskeleton organization
response to lipopolysaccharide
intracellular signal transduction
positive regulation of catalytic activity
cell chemotaxis
chemokine-mediated signaling pathway
positive regulation of neutrophil chemotaxis
response to amphetamine
response to molecule of bacterial origin
acute inflammatory response
positive regulation of leukocyte chemotaxis
positive regulation of cytosolic calcium ion concentration
response to heat
response to gamma radiation
positive regulation of sodium ion transport
neutrophil chemotaxis
response to estradiol
positive regulation of superoxide anion generation
regulation of cell proliferation
positive regulation of potassium ion transport
response to glucocorticoid
positive regulation of neutrophil mediated killing of fungus
positive regulation of hematopoietic stem cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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