AA Sequence of recombinant protein: IPLARTVRCN CIHIDDGPVR MRAIGKLEII PASLSCPRVE IIATMKKNDE QRCLNPESKT IKNLMKAFSQ KRSKRAP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine IP-10 (CXCL10). Anti-Murine IP-10 (CXCL10)-specific antibody was purified by affinity chromatography employing an immobilized Murine IP-10 (CXCL10) matrix.
Sandwich ELISA: To detect Murine IP-10 (CXCL10) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine IP-10 (CXCL10) (500-P129Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine IP-10 (CXCL10).
Western Blot: To detect Murine IP-10 (CXCL10) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine IP-10 (CXCL10) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
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Protein Aliases: 10 kDa interferon gamma-induced protein; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 10; C7; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; Gamma-IP10; H-IP-10; interferon activated gene 10; Interferon-gamma induced protein CRG-2; interferon-gamma-induced protein CRG-2; M-IP-10; small inducible cytokine B subfamily (Cys-X-Cys), member 10; Small-inducible cytokine B10
Gene Aliases: C7; CRG-2; Crg2; Cxcl10; gIP-10; Ifi10; INP10; IP-10; IP10; mob-1; Scyb10
UniProt ID: (Mouse) P17515
Entrez Gene ID: (Mouse) 15945
Molecular Function: chemokine