Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
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AA Sequence of recombinant protein: VPLSRTVRCT CISISNQPVN PRSLEKLEII PASQFCPRVE IIATMKKKGE KRCLNPESKA IKNLLKAVSK ERSKRSP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IP-10 (CXCL10). Anti-Human IP-10 (CXCL10)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA: To detect hIP-10 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IP-10 (500-P93) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hIP-10.
Western Blot: To detect hIP-10 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hIP-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
500-P93BT-1MG will be provided as 2 x 500 µg
IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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