Invitrogen

CXCL10 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Neutralization to ensure that the antibody binds to the antigen stated. View Details

View all (23) CXCL10 (IP-10) antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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CXCL10 Antibody in Immunocytochemistry (ICC) — Immunocytochemical analysis of CXCL10/IP-10 was detected in immersion fixed PHA-treated human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL human CXCL10/IP-10 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46999) for 3 hours at room temperature. Cells were stained with the 557-conjugated Anti-Goat IgG Secondary Antibody (re and counterstained with DAPI (blue).

CXCL10 Antibody in Neutralization (Neu) — Neutralization assay of recombinant human CXCL10/IP-10 chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by recombinant human CXCL10/IP-10 (0.2 µg/mL) is neutralized (green line) by increasing concentrations of human CXCL10/IP-10 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46999). The ND50 is typically 1-4 µg/mL.

CXCL10 Antibody in Neutralization — Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of CXCL10 is shown by the decrease in mean RFU (relative fluorescent units - measured by Resazurin - measure of chemotaxis) with increasing concentrations of CXCL10 Polyclonal Antibody (PA5-46999). {Neu}

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

5-15 µg/mL

Immunofluorescence (IF)

5-15 µg/mL

Flow Cytometry (Flow)

2.5 µg/1x10^6 cells

Neutralization (Neu)

1-4 µg/mL

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant human CXCL10/IP-10/CRG-2

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2609777

Product Specific Information

In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse CRG-2, recombinant human (rh) MIG, and rhGRO gamma.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.20 EU per 1 µg of the antibody by the LAL method.

Target Information

IP-10 (interferon-gamma induced protein 10 kD; in mouse, also called cytokine responsive gene 2, Crg-2, or mob-1) is a highly inducible, primary response gene that belongs to the CXC chemokine superfamily. It was first cloned in 1985. The biological functions of IP-10 are still unclear. Like Mig, IP-10 has no activity on neutrophils. Its functions include stimulation of monocytes, natural killer and T-cell migration, regulation of T-cell and bone marrow progenitor maturation, modulation of adhesion molecule expression as well as inhibition of angiogenesis. Mig and IP-10 share the same receptor, CXCR3.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 10 kDa interferon gamma-induced protein; C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 10; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 10; CXC; CXC motif chemokine; CXCL; CXCL10(1-73); gamma IP10; Gamma-IP10; H-IP-10; interferon-inducible cytokine IP-10; M-IP-10; protein 10 from interferon (gamma)-induced cell line; small inducible cytokine subfamily B (Cys-X-Cys), member 10; Small-inducible cytokine B10

Gene Aliases: C7; crg-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10

UniProt ID: (Human) P02778

Entrez Gene ID: (Human) 3627

Molecular Function: chemokine cytokine signaling molecule