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CXCL16 Antibody (500-P200-100UG) in WB
Western Blot: To detect Human CXCL16 by Western Blot analysis CXCL16 Polyclonal Antibody (Product # 500-P200-1MG) can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P200-100UG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Human CXCL16
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2929783
Product Specific Information
AA Sequence of recombinant protein: NEGSVTGSCY CGKRISSDSP PSVQFMNRLR KHLRAYHRCL YYTRFQLLSW SVCGGNKDPW VQELMSCLDL KECGHAYSGI VAHQKHLLP.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human CXCL16. Anti-Human CXCL16-specific antibody was purified by affinity chromatography employing an immobilized Human CXCL16 matrix.
Sandwich ELISA: To detect Human CXCL16 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human CXCL16 (500-P200Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human CXCL16.
Western Blot: To detect Human CXCL16 by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human CXCL16 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
CXCL16 is a member of the CXC chemokine family and signals through the CXCR6 receptor. CXCL16 may play a role in attracting lymphocyte subsets during inflammation and may facilitate certain immune responses. The chemokine domain of CXCL16 contains six cysteine residues, including the four highly conserved cysteine residues characteristic of CXC chemokines. The CXCL16 gene codes for a 273 amino acid polypeptide, which includes a 29 amino acid cytoplasmic domain and transmembrane sequence containing approximately 20 amino acids. The extracellular portion of CXCL16 contains a chemokines domain and an extended C-terminal mucin-like stalk sequence. The extracellular domain contains 89 amino acid residues (86 a.a. residues for the murine homolog).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 16; CC motif chemokine; CCmotif chemokine; chemokine (C-X-C motif) ligand 16; CXC; CXC chemokine ligand 16; CXC motif chemokine; CXCL; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; Small-inducible cytokine B16; SR-PSOX; Transmembrane chemokine CXCL16
Gene Aliases: CXCL16; CXCLG16; SCYB16; SR-PSOX; SRPSOX; UNQ2759/PRO6714
UniProt ID: (Human) Q9H2A7
Entrez Gene ID: (Human) 58191
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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