Invitrogen

CXCL5 Polyclonal Antibody

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CXCL5 Antibody in Neutralization (Neu) — Neutralization assay of recombinant Rat CXCL5/LIX chemo-attracts the BaF3 mouse pro-B cell line transfected with human CXCR2 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by recombinant Rat CXCL5/LIX (0.2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CXCL5/LIX Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47738). The ND50 is typically 0.3-1.5 µg/mL.

Product Details

Tested Applications

Dilution

Neutralization (Neu)

0.3-1.5 µg/mL

Western Blot (WB)

0.1 µg/mL

Product Specifications

Species Reactivity

Rat

Host / Isotype

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived recombinant rat LIX Ala38-Gln130

Conjugate

Unconjugated

Form

Lyophilized

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5% trehalose

Contains

No Preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2608217

Product Specific Information

In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse GCP-2 is observed and less than 5% cross-reactivity with recombinant human GCP-2 is observed.

Reconstitute at 0.2 mg/mL in sterile PBS.

Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.

Target Information

CXCL5 is a member of the CXC subfamily of chemokines. The cDNA encodes a 114 amino acid precursor protein which when cleaved generates a 36 amino acid signal peptide and a 78 amino acid mature protein which contains a 12 amino acids sequence found before the CXC motif. CXCL5 is produced by various human epithelial cells and it binds to the same receptors as IL8 and NAP2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-X-C motif chemokine 5; chemokine (C-X-C motif) ligand 5; chemokine (C-X-C motif) ligand 6 (granulocyte chemotactic protein 2); CXC chemokine LIX; Cytokine LIX; ENA-78; LIX; Scyb5; Scyb6; Small-inducible cytokine B5

Gene Aliases: Cxcl5; Scyb5

UniProt ID: (Rat) P97885

Entrez Gene ID: (Rat) 60665

Molecular Function: chemokine cytokine signaling molecule