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Invitrogen
CXCR4 Polyclonal Antibody
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FIGURE: 1 / 14
CXCR4 Antibody (PA3-305) in ICC/IF
Immunofluorescent analysis of CXCR4 (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CXCR4 polyclonal antibody (Product # PA3-305) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugate... View More
Product Details
PA3-305
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Marsupial,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues K(338) G K R G G H S S V S T E S E S S S F H S S (359) of human CXCR4 receptor protein.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Several different molecular weight isoforms of CXCR4 have been described in the literature, which vary depending on cell type tested and extraction buffer used. Using Ramos, Raji, and Jurkat membrane fractions, two different MW forms of CXCR4 were detected with PA3-305 by Western blot, between 55-72kD.
It is recommended to use a membrane isolation buffer/kit for extracting CXCR4 for Western blot analysis. Cellular membrane fractions were isolated for Western blot using Mem-PER Plus (Product # 89842).
Target Information
CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C) receptor 4; chemokine receptor 4; CXC; CXC motif chemokine; CXC-R4; CXCR-4; FB22; Fusin; fusin; G protein-coupled receptor; HUMSTR; LESTR; G protein-coupled receptor; G-protein coupled receptor; HM89; HUMSTR; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; Lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPYRL; PB-CKR; Pre-B-cell-derived chemokine receptor; SDF-1 receptor; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor; unnamed protein product
Gene Aliases: b2b220Clo; CD184; Cmkar4; CXCR4; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; PB-CKR; PBSF/SDF-1; Sdf1r; WHIM; WHIMS; WHIMS1
UniProt ID: (Human) P61073, (Mouse) P70658
Entrez Gene ID: (Human) 7852, (Mouse) 12767
virus receptor activity
actin binding
G-protein coupled receptor activity
chemokine receptor activity
protein binding
coreceptor activity
C-C chemokine receptor activity
C-X-C chemokine receptor activity
cytokine binding
C-C chemokine binding
ubiquitin protein ligase binding
myosin light chain binding
C-X-C motif chemokine 12 receptor activity
ubiquitin binding
signal transducer activity
drug binding
patterning of blood vessels
ameboidal-type cell migration
neuron migration
epithelial cell development
chemotaxis
signal transduction
G-protein coupled receptor signaling pathway
germ cell development
nervous system development
brain development
motor neuron axon guidance
germ cell migration
organ morphogenesis
cell migration
calcium-mediated signaling
telencephalon cell migration
regulation of cell migration
T cell proliferation
neutrophil activation
regulation of programmed cell death
myelin maintenance
positive regulation of oligodendrocyte differentiation
regulation of chemotaxis
regulation of calcium ion transport
cardiac muscle contraction
chemokine-mediated signaling pathway
cellular response to cytokine stimulus
response to hypoxia
dendritic cell chemotaxis
apoptotic process
inflammatory response
immune response
adenylate cyclase-activating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
response to virus
neurogenesis
regulation of cell adhesion
positive regulation of cell migration
CXCL12-activated CXCR4 signaling pathway
viral entry into host cell
cell chemotaxis
positive regulation of cold-induced thermogenesis
positive regulation of vasculature development
positive regulation of macrophage migration inhibitory factor signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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