Immunofluorescence analysis of CXCR7 was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CXCR7 Rabbit Polyclonal Antibody (Product # PA3-069) at 1:1000 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More

CXCR7 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CXCR7 Antibody — CXCR7 shRNA knocks down CXCR7 mRNA and protein expression levels in melanoma M14 cells. (A) Western blot analysis of CXCR7 protein expression levels in melanoma M14 and A375 cells. β-actin was used as a protein level control. (B) M14 cells had significantly higher CXCR7 protein expression compared with that of A375 cells. The CXCR7/β-actin protein ratio in M14 and A375 cells was 0.649±0.080 and 0.288±0.040, respectively (**P=0.006). (C) CXCR7 shRNA significantly reduced CXCR7 mRNA expression in M14 cells. CXCR7 shRNA knock down was performed in M14 cells. Empty vector-transfected and non-transfected M14 cells were used as controls. Polymerase chain reaction was conducted to monitor CXCR7 mRNA expression. β-actin was used as an internal mRNA level control. The CXCR7/β-actin mRNA ratio was 1.000±0.102 (control), 1.211±0.117 (vector-transfected) and 0.412±0.023 (CXCR7 shRNA-transfected) in M14 cells. (**P=0.007 was obtained for the comparison between vector-transfected and CXCR7 shRNA-transfected cells). (D) Western blot images of CXCR7 and β-actin protein expression levels. (E) CXCR7 shRNA significantly inhibited CXCR7 protein expression in M14 cells. The CXCR7/β-actin protein ratio was 1.000±0.002 (control), 1.016±0.004 (vector) and 0.144±0.005 (CXCR7 shRNA) in the different groups, which confirmed the knockdown of CXCR7 protein expression in M14 cells (***P<0.001 was obtained for the comparison between vector and CXCR7 shRNA groups). CXCR7, C-X-C chemokine receptor type 7; sh {KD}

Immunogen image

Immunogen Image CXCR7 Polyclonal Antibody

Product Details

PA3-069

Applications

Tested Dilution

Publications

Western Blot (WB)

1:4,000-1:6,000

View 3 publications 3 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:1,000

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

COOH-terminal amino acids of mouse CXCR7 (347-362) SRVSETEYSALEQNTK coupled to KLH.

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.02% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2540496

Product Specific Information

PA3-069 detects CXC Chemokine Receptor Type 7 from human and mouse samples.

PA3-069 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects a 42 kDa protein corresponding to mouse CXC Chemokine Receptor Type 7.

The PA3-069 antigen is the COOH-terminal amino acids of mouse CXCR7 (347-362) SRVSETEYSALEQNTK coupled to KLH.

Target Information

This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Atypical chemokine receptor 3; C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 7; CC motif chemokine; CCmotif chemokine; chemokine; chemokine (C-X-C motif) receptor 7; Chemokine orphan receptor 1; CXC; CXC motif chemokine; CXC-R7; CXCR-7; G protein-coupled receptor; G-protein coupled receptor 159; G-protein coupled receptor RDC1 homolog; RDC-1; unnamed protein product

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Gene Aliases: ACKR3; AW541270; CMKOR1; CXC-R7; CXCR-7; CXCR7; GPR159; RDC-1; RDC1

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UniProt ID: (Human) P25106, (Rat) O89039, (Mouse) P56485

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Entrez Gene ID: (Human) 57007, (Rat) 84348, (Mouse) 12778

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Function(s)

G-protein coupled receptor activity scavenger receptor activity protein binding coreceptor activity C-C chemokine receptor activity C-X-C chemokine receptor activity chemokine binding C-C chemokine binding C-X-C chemokine binding signal transducer activity chemokine receptor activity

Process(es)

angiogenesis vasculogenesis chemotaxis cell adhesion G-protein coupled receptor signaling pathway receptor internalization chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage positive regulation of defense response to virus by host signal transduction multicellular organism development immune response positive regulation of cytosolic calcium ion concentration negative regulation of cell proliferation calcium-mediated signaling oculomotor nerve development cell chemotaxis positive regulation of mesenchymal stem cell migration

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CXCR7 Polyclonal Antibody

CXCR7 Antibody (PA3-069) in ICC/IF

Product Details

PA3-069

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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