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CYFIP1 Antibody (PA5-87780) in WB
Western blot analysis of CYFIP1 in extracts of various cell lines. Samples were incubated with CYFIP1 Polyclonal antibody (Product # PA5-87780) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-87780
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CYFIP1 (NP_0554231)
Conjugate
Form
Liquid
Concentration
0.77 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2804403
Product Specific Information
Immunogen sequence: MAAQVTLEDA LSNVDLLEEL PLPDQQPCIE PPPSSLLYQP NFNTNFEDRN AFVTGIARYI EQATVHSSMN EMLEEGQEYA VMLYTWRSCS RAIPQVKCNE QPNRVEIYEK TVEVLEPEVT KLMNFMYFQR NAIERFCGEV RRLCHAERRK DFVSEAYLIT LGKFINMFAV LDELKNMKCS VKNDHSAYKR AAQFLRKMAD PQSIQESQNL SMFLANHNKI TQSLQQQLEV ISGYEELLAD IVNLCVDYYE NRMYLTPSEK HMLLKVMGFG; Positive Samples: B cells, HepG2, HT-1080, Mouse lung, Mouse brain; Cellular Location: Cell junction, Cell projection, Cytoplasm, lamellipodium, perinuclear region, ruffle, synapse, synaptosome
Target Information
Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA. Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CYFIP1; Cytoplasmic FMR1-interacting protein 1; cytoplasmic FMRP interacting protein 1; FLJ45151; p140sra-1; selective hybridizing clone; Specifically Rac1-associated protein 1; Sra-1
Gene Aliases: CYFIP1; E030028J09Rik; KIAA0068; l(7)1Rl; l71Rl; l7Rl1; mKIAA0068; P140SRA-1; P140sra1; pl-1; SHYC; SRA-1; SRA1
UniProt ID: (Human) Q7L576, (Mouse) Q7TMB8
Entrez Gene ID: (Human) 23191, (Mouse) 20430
regulation of cell shape
Rac protein signal transduction
lamellipodium assembly
ruffle organization
Fc-gamma receptor signaling pathway involved in phagocytosis
vascular endothelial growth factor receptor signaling pathway
axon extension
cognition
positive regulation of Arp2/3 complex-mediated actin nucleation
multicellular organism development
nervous system development
cell differentiation
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