Proteintech

CYP11A1 Polyclonal Antibody

CYP11A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CYP11A1 Antibody (13363-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human placenta tissue slide using 13363-1-AP ( CYP11A1 Antibody) at dilution of 1:200 (under 40x lens).

Product Image Gallery

Product Details

13363-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 29 publications 29 publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CYP11A1 Fusion Protein Ag4182 (1-300 aa encoded by BC032329)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MLAKGLPPR SVLVKGCQTF LSAPREGLGR LRVPTGEGAG ISTRSPRPFN EIPSPGDNGW LNLYHFWRET GTHKVHLHHV QNFQKYGPIY REKLGNVESV YVIDPEDVAL LFKSEGPNPE RFLIPPWVAY HQYYQRPIGV LLKKSAAWKK DRVALNQEVM APEATKNFLP LLDAVSRDFV SVLHRRIKKA GSGNYSGDIS DDLFRFAFES ITNVIFGERQ GMLEEVVNPE AQRFIDAIYQ MFHTSVPMLN LPPDLFRLFR TKTWKDHVAA WDVIFSKADI YTQNFYWELR QKGSVHHDYR G (1-300 aa encoded by BC032329)

Target Information

CYP11A1, also known as cytochrome P450C11A1, cytochrome P450scc and cytochrome P450, subfamily XIA, is an enzyme that catalyzes the first step of steroid biosynthesis under the modulation of cAMP signal. CYP11A1 in steroidogenic cells converts cholesterol to pregnenolone, which is determined by hormonal control of cholesterol availability. Expression of the CYP11A1 gene is controlled by the transcription factor SF-1, and the upstream SF-1 binding site in the CYP11A1 gene is required for hormonal stimulation. c-Jun and SF-1 may act synergistically to activate CYP11A1 gene expression. Both Forskolin and 8-Br-cAMPS elevate CYP11A1 mRNA levels in the interstitial cell monolayer, which has a fully functional adenylate cyclase.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prepubertal exposure to copper oxide nanoparticles induces Leydig cell injury with steroidogenesis disorders in mouse testes.

Biochemical and biophysical research communications

Zheng X,Chen J,Kang L,Wei Y,Wu Y,Hong Y,Wang X,Li D,Shen L,Long C,Wei G,Wu S

Sun Apr 30 00:00:00 UTC 2023

Exosomes derived from BMSCs ameliorate cyclophosphamide-induced testosterone deficiency by enhancing the autophagy of Leydig cells via the AMPK-mTOR signaling pathway.

Asian journal of andrology

Liang HY,Peng F,Pan MJ,Liao SL,Wei C,Wei GY,Xie X,Xue KY,Chen MK,Yang JK,Guo WB,Liu CD,Zhou QZ

Fri Jul 21 00:00:00 UTC 2023

Bisphenol S induces oxidative stress-mediated impairment of testosterone synthesis by inhibiting the Nrf2/HO-1 signaling pathway.

Journal of biochemical and molecular toxicology

Wang YX,Dai W,Li YZ,Wu ZY,Kan YQ,Zeng HC,He QZ

Wed Mar 01 00:00:00 UTC 2023

Epg5 deficiency leads to primary ovarian insufficiency due to WT1 accumulation in mouse granulosa cells.

Autophagy

Liu W,Chen M,Liu C,Wang L,Wei H,Zhang R,Ren Z,Chen Y,Luo M,Zhao J,Jiang H,Gao F,Li W

Wed Feb 01 00:00:00 UTC 2023

Lactoferrin Restores the Deoxynivalenol-Impaired Spermatogenesis and Blood-Testis Barrier Integrity via Improving the Antioxidant Capacity and Modifying the Cell Adhesion and Inflammatory Response.

Antioxidants (Basel, Switzerland)

Li Z,Zhao Y,Zong Q,Hu P,Bao W,Liu HY,Cai D

Mon Jan 09 00:00:00 UTC 2023

Rat Ovarian Function Is Impaired during Experimental Autoimmune Encephalomyelitis.

Cells

Milosevic A,Lavrnja I,Savic D,Milosevic K,Skuljec J,Bjelobaba I,Janjic MM

Thu Mar 30 00:00:00 UTC 2023

Androgens improve ovarian follicle function impaired by glucocorticoids through an androgen-IGF1-FSH synergistic effect.

Frontiers in endocrinology

Gao L,Gao H,Wang W

Tue Nov 08 00:00:00 UTC 2022

Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.

Particle and fibre toxicology

Jin H,Yan M,Pan C,Liu Z,Sha X,Jiang C,Li L,Pan M,Li D,Han X,Ding J

Thu Feb 17 00:00:00 UTC 2022

Paternal cadmium exposure affects testosterone synthesis by reducing the testicular cholesterol pool in offspring mice.

Ecotoxicology and environmental safety

Zhou J,Zhang Y,Zeng L,Wang X,Mu H,Wang M,Pan H,Su P

Thu Sep 01 00:00:00 UTC 2022

In ovo injection of betaine promotes adrenal steroidogenesis in pre-hatched chicken fetuses.

Poultry science

Abobaker H,Omer NA,Hu Y,Idriss AA,Zhao R

Wed Jun 01 00:00:00 UTC 2022

Chronic exposure to propylparaben at the humanly relevant dose triggers ovarian aging in adult mice.

Ecotoxicology and environmental safety

Yan W,Li M,Guo Q,Li X,Zhou S,Dai J,Zhang J,Wu M,Tang W,Wen J,Xue L,Jin Y,Luo A,Wang S

Fri Apr 15 00:00:00 UTC 2022

Effects of Bushen Yiyuan recipe on testosterone synthesis in Leydig cells of rats with exercise-induced low serum testosterone levels.

Pharmaceutical biology

Wang Y,Peng X,Zhou Z,Tang C,Liu W

Thu Dec 01 00:00:00 UTC 2022

The neurosteroid pregnenolone is synthesized by a mitochondrial P450 enzyme other than CYP11A1 in human glial cells.

The Journal of biological chemistry

Lin YC,Cheung G,Porter E,Papadopoulos V

Fri Jul 01 00:00:00 UTC 2022

The neurosteroid pregnenolone is synthesized by a mitochondrial P450 enzyme other than CYP11A1 in human glial cells.

The Journal of biological chemistry

Lin YC,Cheung G,Porter E,Papadopoulos V

Fri Jul 01 00:00:00 UTC 2022

Low-Intensity Pulsed Ultrasound Alleviates Human Testicular Leydig Cell Senescence In Vitro.

International journal of molecular sciences

Han S,Luo J,Xu S,Zhao L,Yao C,Xu J,Tian R,Zhi E,Huang Y,Xia S,Li Z,Li P

Tue Dec 27 00:00:00 UTC 2022

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Clinical and translational medicine

Zhou L,Zhang C,Yang X,Liu L,Hu J,Hou Y,Tao H,Sugimura H,Chen Z,Wang L,Chen K

Tue Jun 01 00:00:00 UTC 2021

Melatonin inhibits lipid accumulation to repress prostate cancer progression by mediating the epigenetic modification of CES1.

Clinical and translational medicine

Zhou L,Zhang C,Yang X,Liu L,Hu J,Hou Y,Tao H,Sugimura H,Chen Z,Wang L,Chen K

Tue Jun 01 00:00:00 UTC 2021

PRMT5 regulates ovarian follicle development by facilitating Wt1 translation.

eLife

Chen M,Dong F,Chen M,Shen Z,Wu H,Cen C,Cui X,Bao S,Gao F

Fri Aug 27 00:00:00 UTC 2021

PRMT5 regulates ovarian follicle development by facilitating Wt1 translation.

eLife

Chen M,Dong F,Chen M,Shen Z,Wu H,Cen C,Cui X,Bao S,Gao F

Fri Aug 27 00:00:00 UTC 2021

Proteomics analysis reveals the effect of 1α,25(OH)₂VD₃-glycosides on development of early testes in piglets.

Scientific reports

Chen H,Bühler K,Zhu Y,Nie X,Liu W

Mon May 31 00:00:00 UTC 2021

Cadmium attenuates testosterone synthesis by promoting ferroptosis and blocking autophagosome-lysosome fusion.

Free radical biology & medicine

Zeng L,Zhou J,Wang X,Zhang Y,Wang M,Su P

Sat Nov 20 00:00:00 UTC 2021

Prenatal exposure to propylparaben at human-relevant doses accelerates ovarian aging in adult mice.

Environmental pollution (Barking, Essex : 1987)

Li M,Zhou S,Wu Y,Li Y,Yan W,Guo Q,Xi Y,Chen Y,Li Y,Wu M,Zhang J,Wei J,Wang S

Wed Sep 15 00:00:00 UTC 2021

An integrated RNA-Seq and network study reveals that valproate inhibited progesterone production in human granulosa cells.

The Journal of steroid biochemistry and molecular biology

Li S,Qi J,Sun Y,Gao X,Ma J,Zhao S

Mon Nov 01 00:00:00 UTC 2021

Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells.

Cell death & disease

Jing J,Ding N,Wang D,Ge X,Ma J,Ma R,Huang X,Jueraitetibaike K,Liang K,Wang S,Cao S,Zhao AZ,Yao B

Fri Aug 14 00:00:00 UTC 2020

CYP11A1 Upregulation Leads to Trophoblast Oxidative Stress and Fetal Neurodevelopmental Toxicity That can be Rescued by Vitamin D.

Frontiers in molecular biosciences

Wang X,Li M,Zhang X,Li Y,He G,Dinnyés A,Sun Q,Xu W

Fri Mar 05 00:00:00 UTC 2021

Genipin improves reproductive health problems caused by circadian disruption in male mice.

Reproductive biology and endocrinology : RB&E

Xu Y,Wang L,Cao S,Hu R,Liu R,Hua K,Guo Z,Di HJ,Hu Z

Fri Dec 11 00:00:00 UTC 2020

La₂O₃ Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice.

International journal of nanomedicine

Yuan L,Li Q,Bai D,Shang X,Hu F,Chen Z,An T,Chen Y,Zhang X

Thu Jul 23 00:00:00 UTC 2020

Bisphenol A decreases progesterone synthesis in human ovarian granulosa cells.

Birth defects research

Qi J,Liu L,Yang J,Gao X,Zhang W

Tue Dec 01 00:00:00 UTC 2020

Proteomic analysis of mouse ovaries during the prepubertal stages.

Experimental cell research

Xiong J,Wu M,Zhang Q,Zhang C,Xiong G,Ma L,Lu Z,Wang S

Mon Apr 15 00:00:00 UTC 2019

The ovarian estrogen synthesis function was impaired in Y123F mouse and partly restored by exogenous FSH supplement.

Reproductive biology and endocrinology : RB&E

Tu X,Liu M,Tang J,Zhang Y,Shi Y,Yu L,Sun Z

Fri May 04 00:00:00 UTC 2018

The ovarian estrogen synthesis function was impaired in Y123F mouse and partly restored by exogenous FSH supplement.

Reproductive biology and endocrinology : RB&E

Tu X,Liu M,Tang J,Zhang Y,Shi Y,Yu L,Sun Z

Fri May 04 00:00:00 UTC 2018

The ovarian estrogen synthesis function was impaired in Y123F mouse and partly restored by exogenous FSH supplement.

Reproductive biology and endocrinology : RB&E

Tu X,Liu M,Tang J,Zhang Y,Shi Y,Yu L,Sun Z

Fri May 04 00:00:00 UTC 2018

The ovarian estrogen synthesis function was impaired in Y123F mouse and partly restored by exogenous FSH supplement.

Reproductive biology and endocrinology : RB&E

Tu X,Liu M,Tang J,Zhang Y,Shi Y,Yu L,Sun Z

Fri May 04 00:00:00 UTC 2018

Mitochondrial Function Regulated by Mitoguardin-1/2 Is Crucial for Ovarian Endocrine Functions and Ovulation.

Endocrinology

Liu XM,Zhang YL,Ji SY,Zhao LW,Shang WN,Li D,Chen Z,Tong C,Fan HY

Wed Nov 01 00:00:00 UTC 2017

Mitochondrial Function Regulated by Mitoguardin-1/2 Is Crucial for Ovarian Endocrine Functions and Ovulation.

Endocrinology

Liu XM,Zhang YL,Ji SY,Zhao LW,Shang WN,Li D,Chen Z,Tong C,Fan HY

Wed Nov 01 00:00:00 UTC 2017

An Outer Mitochondrial Translocase, Tom22, Is Crucial for Inner Mitochondrial Steroidogenic Regulation in Adrenal and Gonadal Tissues.

Molecular and cellular biology

Rajapaksha M,Kaur J,Prasad M,Pawlak KJ,Marshall B,Perry EW,Whittal RM,Bose HS

Tue Jan 19 00:00:00 UTC 2016

An Outer Mitochondrial Translocase, Tom22, Is Crucial for Inner Mitochondrial Steroidogenic Regulation in Adrenal and Gonadal Tissues.

Molecular and cellular biology

Rajapaksha M,Kaur J,Prasad M,Pawlak KJ,Marshall B,Perry EW,Whittal RM,Bose HS

Tue Jan 19 00:00:00 UTC 2016

Ovarian-like differentiation in eutopic and ectopic endometrioses with aberrant FSH receptor, INSL3 and GATA4/6 expression.

BBA clinical

Fouquet B,Santulli P,Noel JC,Misrahi M

Thu Dec 01 00:00:00 UTC 2016

Ovarian-like differentiation in eutopic and ectopic endometrioses with aberrant FSH receptor, INSL3 and GATA4/6 expression.

BBA clinical

Fouquet B,Santulli P,Noel JC,Misrahi M

Thu Dec 01 00:00:00 UTC 2016

1,25D3 prevents CD8(+)Tc2 skewing and asthma development through VDR binding changes to the Cyp11a1 promoter.

Nature communications

Schedel M,Jia Y,Michel S,Takeda K,Domenico J,Joetham A,Ning F,Strand M,Han J,Wang M,Lucas JJ,Vogelberg C,Kabesch M,O'Connor BP,Gelfand EW

Mon Jan 11 00:00:00 UTC 2016

1,25D3 prevents CD8(+)Tc2 skewing and asthma development through VDR binding changes to the Cyp11a1 promoter.

Nature communications

Schedel M,Jia Y,Michel S,Takeda K,Domenico J,Joetham A,Ning F,Strand M,Han J,Wang M,Lucas JJ,Vogelberg C,Kabesch M,O'Connor BP,Gelfand EW

Mon Jan 11 00:00:00 UTC 2016

Mitochondria-associated endoplasmic reticulum membrane (MAM) regulates steroidogenic activity via steroidogenic acute regulatory protein (StAR)-voltage-dependent anion channel 2 (VDAC2) interaction.

The Journal of biological chemistry

Prasad M,Kaur J,Pawlak KJ,Bose M,Whittal RM,Bose HS

Fri Jan 30 00:00:00 UTC 2015

Pregnenolone can protect the brain from cannabis intoxication.

Science (New York, N.Y.)

Vallée M,Vitiello S,Bellocchio L,Hébert-Chatelain E,Monlezun S,Martin-Garcia E,Kasanetz F,Baillie GL,Panin F,Cathala A,Roullot-Lacarrière V,Fabre S,Hurst DP,Lynch DL,Shore DM,Deroche-Gamonet V,Spampinato U,Revest JM,Maldonado R,Reggio PH,Ross RA,Marsicano G,Piazza PV

Fri Jan 03 00:00:00 UTC 2014

Pregnenolone can protect the brain from cannabis intoxication.

Science (New York, N.Y.)

Fri Jan 03 00:00:00 UTC 2014

Immunohistochemical Characterization of S100A6 in the Murine Ovary.

Acta histochemica et cytochemica

Hanaue M,Miwa N,Takamatsu K

Wed Feb 29 00:00:00 UTC 2012

Immunohistochemical Characterization of S100A6 in the Murine Ovary.

Acta histochemica et cytochemica

Hanaue M,Miwa N,Takamatsu K

Wed Feb 29 00:00:00 UTC 2012

Bioinformatics

Protein Aliases: cholesterol 20-22 desmolase; Cholesterol desmolase; cholesterol monooxygenase (side-chain cleaving); cholesterol side chain cleavage; cholesterol side-chain cleavage cytochrome P450; Cholesterol side-chain cleavage enzyme, mitochondrial; CYPXIA1; cytochrome P450 11a cholesterol side chain cleavage; Cytochrome P450 11A1; cytochrome P450 family 11 subfamily A polypeptide 1; cytochrome P450 side-chain cleavage enzyme; cytochrome P450 subfamily 11A; Cytochrome P450(scc); cytochrome P450, 11a, cholesterol side-chain cleavage; cytochrome P450, family 11, subfamily A, polypeptide 1; cytochrome P450, subfamily XIA (cholesterol side chain cleavage); cytochrome P450C11A1; P450(scc); steroid 20-22-lyase

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Gene Aliases: cscc; CYP11A; Cyp11a-1; CYP11A1; CYPXIA1; D9Ertd411e; P450SCC; Scc

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UniProt ID: (Human) P05108, (Rat) P14137, (Mouse) Q9QZ82

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Entrez Gene ID: (Human) 1583, (Rat) 29680, (Mouse) 13070

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Function(s)

iron ion binding cholesterol monooxygenase (side-chain-cleaving) activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen heme binding cholesterol binding monooxygenase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen metal ion binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CYP11A1 Polyclonal Antibody

CYP11A1 Antibody (13363-1-AP) in IHC (P)

Product Details

13363-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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