Invitrogen

CYP24A1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (8) CYP24A1 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

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CYP24A1 Antibody in Western Blot (WB) — Western blot analysis of CYP24A1 using 30µg of A) 293T (B) A431 (C) H1299 (D) HeLa S3 (E) HepG2 (F) MOLT4 and G) Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CYP24A1 polyclonal antibody (Product # PA5-21704) at a dilution of 1:3000.

CYP24A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CYP24A1 in the cytosol of paraffin-embedded mouse kidney using a CYP24A1 polyclonal antibody (Product # PA5-21704) at a dilution of 1:500.

CYP24A1 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell and tissue lysates owing to their inherent genetic constitution. Relatively low expression of CYP24A1 was observed in Mouse Skeletal Muscle when compared to other tissues tested using CYP24A1 Polyclonal antibody (Product # PA5-21704) in western blot. {RE}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 241 and 514 of CYP24A1 (Uniprot ID#Q07973)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11154312

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.

Predicted reactivity: Mouse (87%), Rat (88%), Pig (90%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 1,25-@dihydroxyvitamin D3 24-hydroxylase; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; 25-hydroxyvitamin D-24-hydroxylase; Cytochrome P450 24A1; cytochrome P450, 24; cytochrome P450, 24a1; cytochrome P450, family 24, subfamily A, polypeptide 1; cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase); Cytochrome P450-CC24; exo-mitochondrial protein; MGC126273; MGC126274; vitamin D 24-hydroxylase; vitamin D(3) 24-hydroxylase

Gene Aliases: 24-OHase; CP24; Cyp-24; CYP24; CYP24A1; HCAI; HCINF1; P450-CC24

UniProt ID: (Human) Q07973, (Mouse) Q64441

Entrez Gene ID: (Human) 1591, (Mouse) 13081

Molecular Function: oxidoreductase oxygenase