Invitrogen

CYP24A1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

View all (5) CYP24A1 antibodies

Datasheet

Datasheet

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CYP24A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CYP24A1 in the cytosol of paraffin-embedded mouse kidney using a CYP24A1 polyclonal antibody (Product # PA5-21704) at a dilution of 1:500.

CYP24A1 Antibody in Western Blot (WB) — Western blot analysis was performed on tissue extracts of Mouse Kidney (Lane 1), Mouse Bladder (Lane 2), Mouse Lung (Lane 3), Mouse Liver (Lane 4), Mouse Skeletal Muscle (Lane 5) and Mouse Adipose (Lane 6). The blot was probed with Anti-CYP24A1 Polyclonal Antibody (Product # PA5-21704, 1:3000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 59 kDa band corresponding to CYP24A1 was detected across tissues tested and the protein levels were found to be low in Mouse Skeletal Muscle when compared to other tissues.

CYP24A1 Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell and tissue lysates owing to their inherent genetic constitution. Relatively low expression of CYP24A1 was observed in Mouse Skeletal Muscle when compared to other tissues tested using CYP24A1 Polyclonal antibody (Product # PA5-21704) in western blot. {RE}

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 241 and 514 of CYP24A1 (Uniprot ID#Q07973)

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11154312

Product Specific Information

PA5-21704 targets CYP24A1 in WB and IHC-P applications and shows reactivity with Human samples.

The PA5-21704 immunogen is a recombinant fragment corresponding to a region within amino acids 241 and 514 of CYP24A1 (Uniprot ID#Q07973).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.

Target Information

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 1; 1,25-@dihydroxyvitamin D3 24-hydroxylase; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; 25-@dihydroxyvitamin D3 24-hydroxylase; 25-dihydroxyvitamin D(3) 24-hydroxylase; 25-hydroxyvitamin D-24-hydroxylase; CP24; CYP24; cytochrome P450; Cytochrome P450 24A1; cytochrome P450, 24; cytochrome P450, 24a1; cytochrome P450, family 24, subfamily A, polypeptide 1; cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase); Cytochrome P450-CC24; exo-mitochondrial protein; HCAI; mitochondrial; P450-CC24; subfamily XXIV (vitamin D 24-hydroxylase); vitamin D 24-hydroxylase; vitamin D(3) 24-hydroxylase

Gene Aliases: 24-OHase; CP24; Cyp-24; CYP24; CYP24A1; HCAI; HCINF1; P450-CC24

UniProt ID: (Human) Q5I2W7, (Mouse) Q64441

Entrez Gene ID: (Human) 1591, (Mouse) 13081

Molecular Function: oxidoreductase oxygenase