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Invitrogen
CYP2A6 Polyclonal Antibody
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FIGURE: 1 / 2
CYP2A6 Antibody (PA1-342) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1) and Mouse Pancreas (Lane 2). The blots were probed with Anti-Cytochrome P450 2A6 Rabbit polyclonal Antibody (Product # PA1-342, 1:1000-1:5000 dilution dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 56 kDa band corresponding to Cytochrome P450 2A6 was observed in Hep G2 but not in Mouse Pancreas. Known quantity of protein samples were electrophore... View More
Product Details
PA1-342
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified, His-tagged, full length human P450 2A6 fusion protein.
Conjugate
Form
Liquid
Purification
Ammonium sulfate precipitation
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-342 detects cytochrome P450 (P450) 2A6 from human, mouse and rat samples. This antibody detects, to a lesser extent, other P450 isoforms.
PA1-342 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~49 kDa protein representing P450 2A6 from rat liver extract.
The PA1-342 immunogen is purified, His-tagged, full length human P450 2A6 fusion protein.
Target Information
This gene, CYP2A6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to hydroxylate coumarin, and also metabolizes nicotine, aflatoxin B1, nitrosamines, and some pharmaceuticals. Individuals with certain allelic variants are said to have a poor metabolizer phenotype, meaning they do not efficiently metabolize coumarin or nicotine. This gene is part of a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q. The gene was formerly referred to as CYP2A3; however, it has been renamed CYP2A6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 1,4-cineole 2-exo-monooxygenase; Coumarin 7-hydroxylase; CYP; CYPIIA6; Cytochrome P450 2A6; Cytochrome P450 IIA3; Cytochrome P450(I); cytochrome P450, family 2, subfamily A, polypeptide 6; cytochrome P450, subfamily IIA (phenobarbital-inducible), polypeptide 6; flavoprotein-linked monooxygenase; P450; P450 IIA3; P450(I); xenobiotic monooxygenase
Gene Aliases: CPA6; CYP2A; CYP2A3; CYP2A6; CYPIIA6; P450C2A; P450PB
UniProt ID: (Human) P11509
Entrez Gene ID: (Human) 1548
iron ion binding
coumarin 7-hydroxylase activity
arachidonic acid epoxygenase activity
steroid hydroxylase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
enzyme binding
heme binding
oxidoreductase
oxygenase
metabolite interconversion enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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