Proteintech

CYP2E1 Polyclonal Antibody

View all (25) CYP2E1 antibodies

Questions & Answers

Questions & Answers

Product Image Gallery

CYP2E1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

CYP2E1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using CYP2E1 antibody 19937-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

CYP2E1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CYP2E1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

CYP2E1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 19937-1-AP (CYP2E1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

CYP2E1 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 19937-1-AP ( CYP2E1-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 28 publications 28 publications

Immunohistochemistry (IHC)

-

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

-

Immunocytochemistry (ICC/IF)

1:10-1:100

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated

Form

Liquid

Concentration

0.61 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

CYP2E1 can be detected as 50-57 kDa (full-length) and ~40 kDa (N-terminal signal peptide cleaved). The antibody is specific to CYP2E1.

Target Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 4-nitrophenol 2-hydroxylase; CP2E1; CYPIIE1; Cytochrome P450 2E1; cytochrome P450 subfamily 2e1 (ethanol-inducible); cytochrome P450, 2e1, ethanol inducible; cytochrome P450, family 2, subfamily E, polypeptide 1; cytochrome P450, subfamily 2E, polypeptide 1; cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1; Cytochrome P450-ALC; Cytochrome P450-J; Cytochrome P450RLM6; flavoprotein-linked monooxygenase; microsomal monooxygenase; P450-J; P450RLM6; xenobiotic monooxygenase

Gene Aliases: CPE1; CYP2E; Cyp2e-1; CYP2E1; P450-J; P450C2E

UniProt ID: (Human) P05181, (Mouse) Q05421, (Rat) P05182

Entrez Gene ID: (Human) 1571, (Mouse) 13106, (Rat) 25086

Molecular Function: oxidoreductase oxygenase metabolite interconversion enzyme

triglyceride metabolic process xenobiotic metabolic process steroid metabolic process response to ozone response to organonitrogen compound monoterpenoid metabolic process drug metabolic process carbon tetrachloride metabolic process benzene metabolic process epoxygenase P450 pathway halogenated hydrocarbon metabolic process response to drug response to ethanol heterocycle metabolic process oxidation-reduction process exogenous drug catabolic process

Process(es)

monooxygenase activity iron ion binding arachidonic acid epoxygenase activity steroid hydroxylase activity oxidoreductase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen oxygen binding enzyme binding heme binding 3-(3-hydroxyphenyl)propionate hydroxylase activity oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen 4-chlorobenzaldehyde oxidase activity 3,5-xylenol methylhydroxylase activity phenylacetate hydroxylase activity 4-nitrophenol 4-monooxygenase activity dimethyl sulfide monooxygenase activity alpha-pinene monooxygenase [NADH] activity phenanthrene 9,10-monooxygenase activity 1-hydroxy-2-naphthoate hydroxylase activity toluene 4-monooxygenase activity xylene monooxygenase activity dibenzothiophene monooxygenase activity 6-hydroxy-3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity chlorophenol 4-monooxygenase activity carbon disulfide oxygenase activity toluene 2-monooxygenase activity 1-hydroxy-2-oxolimonene 1,2-monooxygenase activity phenanthrene 1,2-monooxygenase activity tetrahydrofuran hydroxylase activity styrene monooxygenase activity toluene-4-sulfonate monooxygenase activity toluene-sulfonate methyl-monooxygenase activity 3-methyl-2-oxo-1,2-dihydroquinoline 6-monooxygenase activity 2-hydroxy-phenylacetate hydroxylase activity 2-oxo-delta3-4,5,5-trimethylcyclopentenylacetyl-CoA 1,2-monooxygenase activity phenanthrene 3,4-monooxygenase activity toluene 3-monooxygenase activity 4-hydroxyphenylacetate,NADH:oxygen oxidoreductase (3-hydroxylating) activity limonene monooxygenase activity 2-methylnaphthalene hydroxylase activity 1-methylnaphthalene hydroxylase activity bisphenol A hydroxylase A activity salicylate 5-hydroxylase activity isobutylamine N-hydroxylase activity branched-chain dodecylbenzene sulfonate monooxygenase activity 3-HSA hydroxylase activity 4-hydroxypyridine-3-hydroxylase activity 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase activity 6-hydroxynicotinate 3-monooxygenase activity metal ion binding tocotrienol omega-hydroxylase activity thalianol hydroxylase activity FMN binding