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Invitrogen
CYP4F2 Polyclonal Antibody
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FIGURE: 1 / 1
CYP4F2 Antibody (PA5-87655) in WB
Western blot analysis of CYP4F2 in extracts of various cell lines. Samples were incubated with CYP4F2 Polyclonal antibody (Product # PA5-87655) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-87655
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 40-120 of human CYP4F2 (NP_0010733)
Conjugate
Form
Liquid
Concentration
2.14 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: AFYDNCRRLR CFPQPPRRNW FWGHQGMVNP TEEGMRVLTQ LVATYPQGFK VWMGPISPLL SLCHPDIIRS VINASAAIAP K; Positive Samples: LO2, HepG2, 293T; Cellular Location: Endoplasmic reticulum membrane, Microsome membrane, Peripheral membrane protein
Target Information
CYP4F isoforms are involved in the oxidation of important cellular mediators, such as leukotriene B4 (LTB4) and prostaglandins. CYP4F2 catalyzes omega-hydroxylation of LTB4 to a less potent proinflammatory eicosanoid, 20-OH-LTB4 as well as arachidonic acid. CYP4F2 is expressed in liver and kidney. Its expression is repressed by peroxisomal proliferators and induced by retinoic acid. X-linked adrenoleukodystrophy (X-ALD) is a severe neurodegenerative disorder biochemically characterized by elevated levels of very long-chain fatty acids (VLCFA). CYP4F2 participates in the omega-hydroxylation of VLCFAs, which may provide an alternative mode of treatment for X-ALD patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 20-HETE synthase; 20-hydroxyeicosatetraenoic acid synthase; Arachidonic acid omega-hydroxylase; CYPIVF2; Cytochrome P450 4F2; cytochrome P450, family 4, subfamily F, polypeptide 2; cytochrome P450, subfamily IVF, polypeptide 2; Cytochrome P450-LTB-omega; Docosahexaenoic acid omega-hydroxylase; Leukotriene-B(4) 20-monooxygenase 1; Leukotriene-B(4) omega-hydroxylase 1; Phylloquinone omega-hydroxylase CYP4F2
Gene Aliases: CPF2; CYP4F2
UniProt ID: (Human) P78329
Entrez Gene ID: (Human) 8529
monooxygenase activity
iron ion binding
protein binding
arachidonic acid epoxygenase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
alkane 1-monooxygenase activity
heme binding
leukotriene-B4 20-monooxygenase activity
arachidonic acid omega-hydroxylase activity
alpha-tocopherol omega-hydroxylase activity
tocotrienol omega-hydroxylase activity
20-hydroxy-leukotriene B4 omega oxidase activity
20-aldehyde-leukotriene B4 20-monooxygenase activity
oxygenase
very long-chain fatty acid metabolic process
long-chain fatty acid metabolic process
renal water homeostasis
pressure natriuresis
icosanoid metabolic process
leukotriene metabolic process
blood coagulation
regulation of blood pressure
drug metabolic process
arachidonic acid metabolic process
epoxygenase P450 pathway
negative regulation of icosanoid secretion
positive regulation of icosanoid secretion
leukotriene B4 catabolic process
vitamin E metabolic process
menaquinone catabolic process
phylloquinone catabolic process
vitamin K catabolic process
sodium ion homeostasis
oxidation-reduction process
omega-hydroxylase P450 pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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