Proteintech

CYP7B1 Polyclonal Antibody

Product Image Gallery

CYP7B1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HEK-293 cells using 24889-1-AP (CYP7B1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

CYP7B1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 24889-1-AP (CYP7B1 antibody) at dilution of 1:200 (under 10x lens).

CYP7B1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 24889-1-AP (CYP7B1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Western Blot (WB)

1:500-1:2000

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CYP7B1 Fusion Protein Ag19374

Conjugate

Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: 24-hydroxycholesterol 7-alpha-hydroxylase; 25-hydroxycholesterol 7-alpha-hydroxylase; 25/26-hydroxycholesterol 7-alpha-hydroxylase; 3-hydroxysteroid 7-alpha hydroxylase; Cytochrome P450 7B1; cytochrome P450, 7b1; cytochrome P450, subfamily VIIB (oxysterol 7 alpha-hydroxylase), polypeptide 1; HCT-1; Hippocampal transcript 1 protein; Oxysterol 7-alpha-hydroxylase

Gene Aliases: AW261589; CBAS3; CP7B; CYP7B1; D3Ertd552e; hct-1; SPG5A

UniProt ID: (Human) O75881, (Mouse) Q60991

Entrez Gene ID: (Human) 9420, (Mouse) 13123

Molecular Function: oxidoreductase oxygenase