Invitrogen

CYTL1 Polyclonal Antibody

View all (4) CYTL1 antibodies

Questions & Answers

Questions & Answers

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CYTL1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CYTL1 in LOVO cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with CYTL1 polyclonal antibody (Product # PA5-101322) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

CYTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded CYTL1 in human Melanoma tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with CYTL1 polyclonal antibody (Product # PA5-101322) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

CYTL1 Antibody in Western Blot (WB) — Western blot analysis of CYTL1 in LOVO cells. Samples were incubated with CYTL1 polyclonal antibody (Product # PA5-101322).

Product Details

PA5-101322

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human CYTL1(Accession Q9NRR1), corresponding to amino acid residues V78-S105. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2850759

Target Information

C17 is a cytokine-like protein specifically expressed in bone marrow and cord blood mononuclear cells that bear the CD34 surface marker (Liu et al., 2000 ).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cytokine like 1; cytokine-like 1; Cytokine-like protein 1; cytokine-like protein C17; Protein C17

Gene Aliases: 4930443F05Rik; 4Cytl1; C17; C4orf4; Cyt1; CYTL1; Gm147; UNQ1942/PRO4425

UniProt ID: (Human) Q9NRR1

Entrez Gene ID: (Human) 54360, (Mouse) 231162

Molecular Function: cytokine intercellular signal molecule

chondrocyte differentiation signal transduction positive regulation of transcription from RNA polymerase II promoter inner ear development chondroitin sulfate proteoglycan biosynthetic process positive regulation of sequence-specific DNA binding transcription factor activity cartilage homeostasis

Process(es)

receptor binding