Invitrogen

CYTL1 Polyclonal Antibody

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CYTL1 Antibody in Western Blot (WB) — Western blot analysis of CYTL1 in LOVO cells. Samples were incubated with CYTL1 polyclonal antibody (Product # PA5-101322).

CYTL1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded CYTL1 in human Melanoma tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with CYTL1 polyclonal antibody (Product # PA5-101322) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

CYTL1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of CYTL1 in LOVO cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C) incubated with CYTL1 polyclonal antibody (Product # PA5-101322) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 at a dilution of 1:600.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC)

1:100-1:500

Immunofluorescence (IF)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human CYTL1, corresponding to a region within the internal amino acids.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2850759

Target Information

C17 is a cytokine-like protein specifically expressed in bone marrow and cord blood mononuclear cells that bear the CD34 surface marker (Liu et al., 2000 ).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cytokine like 1; cytokine-like 1; Cytokine-like protein 1; cytokine-like protein C17; Protein C17

Gene Aliases: 4930443F05Rik; 4Cytl1; C17; C4orf4; Cyt1; CYTL1; Gm147; UNQ1942/PRO4425

UniProt ID: (Human) Q9NRR1

Entrez Gene ID: (Human) 54360, (Mouse) 231162

Function(s)

chondrocyte differentiation positive regulation of transcription from RNA polymerase II promoter inner ear development chondroitin sulfate proteoglycan biosynthetic process positive regulation of sequence-specific DNA binding transcription factor activity cartilage homeostasis signal transduction

Process(es)

receptor binding

CYTL1 Polyclonal Antibody

Product Details

Target Information

References (0)

Bioinformatics

Material safety data sheets (MSDS)

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