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Invitrogen
Calbindin D28K Polyclonal Antibody
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FIGURE: 1 / 8
Calbindin D28K Antibody (PA1-931) in WB
Western blot analysis was performed using whole cell extracts (30 µg) of Mouse Brain (Lane 1), Rat Brain (Lane 2), IMR-32 (Lane 3), SH-SY5Y (Lane 4) and U-2 OS (Lane 5).The blots were probed with Anti-Calbindin D-28K Rabbit Polyclonal Antibody (Product # PA1-931, 2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4µg/mL 1:2500 dilution). A ~28 kDa band corresponding to Calbindin D-28K was observed across tissues and cell lines tested. Known... View More
Product Details
PA1-931
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Cynomolgus monkey,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
28 kDa calbindin-D protein purified from rat kidney.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 15mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
Product Specific Information
PA1-931 detects calbindin D-28k protein in rat samples.
PA1-931 has successfully been used in Western blot , ELISA, immunoprecipitation and immunohistochemical procedures. By Western blot, this antibody detects a 28 kDa protein representing calbindin D from rat cerebellum and rat kidney extract. Immunohistochemical staining on paraffin sections demonstrates that calbindin D is localized to the cytoplasm in epithelial cells of distal tubules in human kidney cortex after staining with PA1-931.
The PA1-931 immunizing protein corresponds to the purified 28 kDa calbindin-D protein from rat kidney.
Reconstitute in 100 µL PBS to create a stock of 1 mg/mL.
Target Information
Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28kDa calcium binding protein, with broad tissue distribution. It belongs, together with calmodulin, S-100, parvalbumin, troponin C and other proteins, to a family of low molecular weight calcium-binding proteins (CaBPs). These CaBPs have homologous primary structures, which contain conserved polypeptide folds of the EF-hand type for Ca2+ binding. Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Calbindin; calbindin 1, (28kD); calbindin 1, 28 kD; calbindin 1, 28kDa; Calbindin D28; calbindin-28K; calbindin-D (28k); CalbindinD28K; cerebellar Ca-binding protein spot 35 protein; cerebellar Ca-binding protein, spot 35 protein; D-28K; PCD-29; RTVL-H protein; Spot 35 protein; Vitamin D-dependent calcium-binding protein, avian-type
Gene Aliases: Brain-2; CAB27; CaBP28K; CALB; Calb-1; CALB1; CB; D-28K
UniProt ID: (Human) P05937, (Rat) P07171, (Mouse) P12658
Entrez Gene ID: (Human) 793, (Rat) 83839, (Mouse) 12307
short-term memory
long-term memory
locomotory behavior
retina layer formation
metanephric part of ureteric bud development
regulation of synaptic plasticity
regulation of cytosolic calcium ion concentration
cellular response to organic substance
metanephric collecting duct development
metanephric distal convoluted tubule development
metanephric connecting tubule development
learning or memory
vitamin D metabolic process
calcium ion homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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