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Invitrogen
Calbindin D28K Polyclonal Antibody
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FIGURE: 1 / 12
Calbindin D28K Antibody (PA5-85669) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Calbindin D28K was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Calbindin D28K Polyclonal Antibody (Product # PA5 85669) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.
Product Details
PA5-85669
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human Calbindin. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.51 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Keep as concentrated solution.
Predicted reactivity: Mouse (97%), Rat (98%), Pig (98%), Rabbit (97%), Chicken (80%), Bovine (100%).
Positive Control: mouse brain, rat brain.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28kDa calcium binding protein, with broad tissue distribution. It belongs, together with calmodulin, S-100, parvalbumin, troponin C and other proteins, to a family of low molecular weight calcium-binding proteins (CaBPs). These CaBPs have homologous primary structures, which contain conserved polypeptide folds of the EF-hand type for Ca2+ binding. Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Calbindin; calbindin 1, (28kD); calbindin 1, 28 kD; calbindin 1, 28kDa; Calbindin D28; calbindin-28K; calbindin-D (28k); CalbindinD28K; cerebellar Ca-binding protein spot 35 protein; cerebellar Ca-binding protein, spot 35 protein; D-28K; PCD-29; RTVL-H protein; Spot 35 protein; Vitamin D-dependent calcium-binding protein, avian-type
Gene Aliases: Brain-2; CAB27; CaBP28K; CALB; Calb-1; CALB1; CB; D-28K
UniProt ID: (Human) P05937, (Rat) P07171, (Mouse) P12658
Entrez Gene ID: (Human) 793, (Rat) 83839, (Mouse) 12307
short-term memory
long-term memory
locomotory behavior
retina layer formation
metanephric part of ureteric bud development
regulation of synaptic plasticity
regulation of cytosolic calcium ion concentration
cellular response to organic substance
metanephric collecting duct development
metanephric distal convoluted tubule development
metanephric connecting tubule development
learning or memory
vitamin D metabolic process
calcium ion homeostasis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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