Proteintech

Calbindin-D28k Polyclonal Antibody

Calbindin-D28k Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Calbindin-D28k Antibody (14479-1-AP) in IHC (P)

IHC result of Calbindin antibody (14479-1-AP, 1:5000 in 1% BSA 0.3%Tx-100 Tris buffer incubated at 4°C overnight) with freshly formalin fixed human hippocampus (floating section). Secondary antibody biotinylated anti-rabbit lgG 1:200 in 1% BSA 0.3%Tx-100 Tris buffer for 2 hours at room temperature.

Calbindin-D28k Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Calbindin-D28k Antibody in Western Blot (WB)

Calbindin-D28k Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14479-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:4,000

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 15 publications 15 publications

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Calbindin-D28k Fusion Protein Ag5861 (109-262 aa encoded by BC006478)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.29 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: KYDTDHSGF IETEELKNFL KDLLEKANKT VDDTKLAEYT DLMLKLFDSN NDGKLELTEM ARLLPVQENF LLKFQGIKMC GKEFNKAFEL YDQDGNGYID ENELDALLKD LCEKNKQDLD INNITTYKKN IMALSDGGKL YRTDLALILC AGDN (109-262 aa encoded by B C006478)

Target Information

Calbindin-D-28K (also termed vitamin D-dependent calcium-binding protein, or cholecalcin), is a highly conserved 28kDa calcium binding protein, with broad tissue distribution. It belongs, together with calmodulin, S-100, parvalbumin, troponin C and other proteins, to a family of low molecular weight calcium-binding proteins (CaBPs). These CaBPs have homologous primary structures, which contain conserved polypeptide folds of the EF-hand type for Ca2+ binding. Calbindin-D-28K is found predominantly in subpopulations of central and peripheral nervous system neurons, and in certain epithelial nervous system neurons, and in certain epithelial cells involved in Ca2+ transport such as distal tubular cells and cortical collecting tubules of the kidney, and in enteric neuroendocrine cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Selenoprotein Gene mRNA Expression Evaluation During Renal Ischemia-Reperfusion Injury in Rats and Ebselen Intervention Effects.

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sEVs^RVG selectively delivers antiviral siRNA to fetus brain, inhibits ZIKV infection and mitigates ZIKV-induced microcephaly in mouse model.

Molecular therapy : the journal of the American Society of Gene Therapy

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Wed May 04 00:00:00 UTC 2022

sEVs^RVG selectively delivers antiviral siRNA to fetus brain, inhibits ZIKV infection and mitigates ZIKV-induced microcephaly in mouse model.

Molecular therapy : the journal of the American Society of Gene Therapy

Zhang R,Fu Y,Cheng M,Ma W,Zheng N,Wang Y,Wu Z

Wed May 04 00:00:00 UTC 2022

Autophagy and post-ischemic conditioning in retinal ischemia.

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Mathew B,Chennakesavalu M,Sharma M,Torres LA,Stelman CR,Tran S,Patel R,Burg N,Salkovski M,Kadzielawa K,Seiler F,Aldrich LN,Roth S

Tue Jun 01 00:00:00 UTC 2021

The long noncoding RNA Synage regulates synapse stability and neuronal function in the cerebellum.

Cell death and differentiation

Wang F,Wang Q,Liu B,Mei L,Ma S,Wang S,Wang R,Zhang Y,Niu C,Xiong Z,Zheng Y,Zhang Z,Shi J,Song X

Wed Sep 01 00:00:00 UTC 2021

Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.

Cell

Zhou H,Su J,Hu X,Zhou C,Li H,Chen Z,Xiao Q,Wang B,Wu W,Sun Y,Zhou Y,Tang C,Liu F,Wang L,Feng C,Liu M,Li S,Zhang Y,Xu H,Yao H,Shi L,Yang H

Thu Apr 30 00:00:00 UTC 2020

Reversing a model of Parkinson's disease with in situ converted nigral neurons.

Nature

Qian H,Kang X,Hu J,Zhang D,Liang Z,Meng F,Zhang X,Xue Y,Maimon R,Dowdy SF,Devaraj NK,Zhou Z,Mobley WC,Cleveland DW,Fu XD

Mon Jun 01 00:00:00 UTC 2020

Paraoxonase 3 functions as a chaperone to decrease functional expression of the epithelial sodium channel.

The Journal of biological chemistry

Shi S,Montalbetti N,Wang X,Rush BM,Marciszyn AL,Baty CJ,Tan RJ,Carattino MD,Kleyman TR

Fri Apr 10 00:00:00 UTC 2020

Identification and immunohistochemical characterization of G-quadruplexes in mouse brain.

Biochemical and biophysical research communications

Asamitsu S,Imai Y,Yabuki Y,Ikenoshita S,Takeuchi M,Kashiwagi H,Tanoue Y,Fukuda T,Shioda N

Thu Oct 08 00:00:00 UTC 2020

Maternal exposure to ibuprofen can affect the programming of the hypothalamus of the male offspring.

Regulatory toxicology and pharmacology : RTP

Balin PDS,Jorge BC,Leite ARR,Borges CS,Oba E,Silva EJR,de Barros AL,Horta-Júnior JAC,Arena AC

Sun Mar 01 00:00:00 UTC 2020

Suppression of microglial activation and monocyte infiltration ameliorates cerebellar hemorrhage induced-brain injury and ataxia.

Brain, behavior, and immunity

Xie ST,Chen AX,Song B,Fan J,Li W,Xing Z,Peng SY,Zhang QP,Dong L,Yan C,Zhang XY,Wang JJ,Zhu JN

Thu Oct 01 00:00:00 UTC 2020

The absence of BBSome function decreases synaptogenesis and causes ectopic synapse formation in the retina.

Scientific reports

Hsu Y,Garrison JE,Seo S,Sheffield VC

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Differentiation of adult human retinal pigment epithelial cells into dopaminergic-like cells in vitro and in the recipient monkey brain.

Molecular medicine (Cambridge, Mass.)

Li S,Zhang H,Wang A,Liu Y,Liu H,Yue F,Abulaiti X,Zhang C,Li L

Fri Mar 29 00:00:00 UTC 2019

Differential effects of N-acetylcysteine on retinal degeneration in two mouse models of normal tension glaucoma.

Cell death & disease

Sano H,Namekata K,Kimura A,Shitara H,Guo X,Harada C,Mitamura Y,Harada T

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Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus.

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Jia F,Zhu X,Lv P,Hu L,Liu Q,Jin S,Xu F

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Notch-mediated Sox9⁺ cell activation contributes to kidney repair after partial nephrectomy.

Life sciences

Ma Q,Wang Y,Zhang T,Zuo W

Mon Jan 15 00:00:00 UTC 2018

Notch-mediated Sox9⁺ cell activation contributes to kidney repair after partial nephrectomy.

Life sciences

Ma Q,Wang Y,Zhang T,Zuo W

Mon Jan 15 00:00:00 UTC 2018

Topical Ripasudil Suppresses Retinal Ganglion Cell Death in a Mouse Model of Normal Tension Glaucoma.

Investigative ophthalmology & visual science

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Topical Ripasudil Suppresses Retinal Ganglion Cell Death in a Mouse Model of Normal Tension Glaucoma.

Investigative ophthalmology & visual science

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Oncology letters

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Bioinformatics

Protein Aliases: Calbindin; calbindin 1, (28kD); calbindin 1, 28 kD; calbindin 1, 28kDa; Calbindin D28; calbindin-28K; calbindin-D (28k); CalbindinD28K; cerebellar Ca-binding protein spot 35 protein; cerebellar Ca-binding protein, spot 35 protein; D-28K; PCD-29; RTVL-H protein; Spot 35 protein; Vitamin D-dependent calcium-binding protein, avian-type

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Gene Aliases: Brain-2; CAB27; CaBP28K; CALB; Calb-1; CALB1; CB; D-28K

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UniProt ID: (Human) P05937, (Rat) P07171, (Mouse) P12658

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Entrez Gene ID: (Human) 793, (Rat) 83839, (Mouse) 12307

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Function(s)

vitamin D binding calcium ion binding protein binding zinc ion binding metal ion binding calmodulin-related

Process(es)

short-term memory long-term memory locomotory behavior retina layer formation metanephric part of ureteric bud development regulation of synaptic plasticity regulation of cytosolic calcium ion concentration cellular response to organic substance metanephric collecting duct development metanephric distal convoluted tubule development metanephric connecting tubule development learning or memory vitamin D metabolic process calcium ion homeostasis

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Calbindin-D28k Polyclonal Antibody

Calbindin-D28k Antibody (14479-1-AP) in IHC (P)

Product Details

14479-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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