Immunofluorescent analysis of Calcium Sensing Receptor (green) showing staining in the cytoplasm and nucleus of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Calcium Sensing Receptor polyclonal antibody (Product # PA1-934A) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues A(12) L A W H S S A Y G P D Q R A Q(27) of rat CaSR.|
|Purification||Antigen affinity chromatography|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-934A detects calcium sensing receptor (CaSR) from human and rat tissues.
PA1-934A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~120 kDa protein representing CaSR from HEK393 cells and rat brain homogenate.
The PA1-934A immunizing peptide corresponds to amino acid residues 12-27 from rat CaSR. This peptide (Cat.# PEP-009) is available for use in neutralization and control experiments.
The calcium sensing receptor (CaSR) has been shown to play a major role in regulating parathyroid hormone secretion and subsequently influencing the calcium concentration of extracellular fluids. Based on sequence analysis of cDNA clones it appears that the CaSR is similar to the 7-transmembrane-domain G-protein-coupled receptor superfamily. Changes in extracellular calcium are thought to modulate a balance between proliferation and differentiation in a variety of cell types. In normal primary keratinocytes and breast epithelial cells, proliferation is inhibited and elevated extracellular calcium levels trigger differentiation. Malignant transformations of these cell types are accompanied by a loss of responsiveness to the anti-proliferative effects of elevated extracellular calcium. Several disorders of calcium homeostasis have been linked to mutations in the CaSR. These include familial hypocalciuric hypercalcemia (FHH), neonatal severe hyperparathyroidism (NSHPT), and autosomal dominant hypocalemia (ADHypo).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Correlation between Choline Peak at MR Spectroscopy and Calcium-Sensing Receptor Expression Level in Breast Cancer: A Preliminary Clinical Study.
PA1-934A was used in immunohistochemistry - paraffin section to correlate choline with the CaSR expression surgical breast specimens
|Baio G,Rescinito G,Rosa F,Pace D,Boccardo S,Basso L,Salvi S,Calabrese M,Truini M,Neumaier CE||Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging (17:548)||2015|
Decreased parathyroid Klotho expression is associated with persistent hyperparathyroidism after kidney transplantation.
PA1-934A was used in immunohistochemistry to study persistent hypoparathyroidism in kidney transplant patients and the role of reduced parathyroid expression of Klotho
|Hong YA,Choi DE,Lim SW,Yang CW,Chang YK||Transplantation proceedings (45:2957)||2013|
In vivo imaging of human breast cancer mouse model with high level expression of calcium sensing receptor at 3T.
PA1-934A was used in immunohistochemistry to evaluate an in vivo breast cancer cell imaging technique
|Baio G,Fabbi M,Emionite L,Cilli M,Salvi S,Ghedin P,Prato S,Carbotti G,Tagliafico A,Truini M,Neumaier CE||European radiology (22:551)||2012|
Establishment and characterization of a novel cell line derived from a human small cell lung carcinoma that secretes parathyroid hormone, parathyroid hormone-related protein, and pro-opiomelanocortin.
PA1-934A was used in immunohistochemistry to characterize a novel hormone-secreting cell line derived from human small cell lung carcinoma
|Ishikawa M,Kimura K,Tachibana T,Hashimoto H,Shimojo M,Ueshiba H,Tsuboi K,Shibuya K,Yoshino G||Human cell (23:58)||2010|
Regulation of mouse lung development by the extracellular calcium-sensing receptor, CaR.
PA1-934A was used in immunohistochemistry to investigate the role of calcium-sensing receptor in mouse lung development
|Finney BA,del Moral PM,Wilkinson WJ,Cayzac S,Cole M,Warburton D,Kemp PJ,Riccardi D||The Journal of physiology (586:6007)||2008|
Normocalcemic primary hyperparathyroidism in patients with recurrent kidney stones: pathological analysis of parathyroid glands.
PA1-934A was used in immunohistochemistry to study the abnormalities in parathyroid tissues from patients with normocalcemic primary hyperparathyroidism
|Yang AH,Hsu CW,Chen JY,Tseng LM,Won GS,Lee CH||Virchows Archiv : an international journal of pathology (449:62)||2006|
A novel germline inactivating mutation in the CASR gene in an Italian kindred affected by familial hypocalciuric hypercalcemia.
PA1-934A was used in western blot to study a novel germline CASR mutation in a case of familial hypocaliuric hypercalcemia in an Italian family
|Falchetti A,Gozzini A,Terranegra A,Soldati L,Vezzoli G,Leoncini G,Giusti F,Franceschelli F,Masi L,Tanini A,Cavalli L,Brandi ML||European journal of endocrinology (166:933)||2012|
The calcium sensing receptor and its alternatively spliced form in keratinocyte differentiation.
PA1-934A was used in western blot to study the functional properties of the calcium-sensing receptor and its alternatively spliced form in keratinocyte differentiation
|Oda Y,Tu CL,Pillai S,Bikle DD||The Journal of biological chemistry (273:23344)||1998|