Invitrogen

Calcium Sensing Receptor Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

Calcium Sensing Receptor Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Calcium Sensing Receptor (green) showing staining in the cytoplasm and nucleus of PC12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Calcium Sensing Receptor polyclonal antibody (Product # PA1-934A) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Calcium Sensing Receptor Antibody in Western Blot (WB) — Western blot analysis of Calcium Sensing Receptor was performed by loading 25 µg of U251 (lane 1) and rat brain (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with a Calcium Sensing Receptor polyclonal antibody (Product # PA1-934A) at a dilution of 1:500 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~120 kDa.

Product Details

PA1-934A

Applications	Tested Dilution	Publications
Western Blot (WB)	1:100	View 3 publications 3 publications
Immunohistochemistry (IHC)	-	View 6 publications 6 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:10-1:100	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to residues A(12) L A W H S S A Y G P D Q R A Q(27) of rat CaSR. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2071477

Product Specific Information

PA1-934A detects calcium sensing receptor (CaSR) from human and rat tissues.

PA1-934A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~120 kDa protein representing CaSR from HEK393 cells and rat brain homogenate.

The PA1-934A immunizing peptide corresponds to amino acid residues 12-27 from rat CaSR. This peptide (Cat.# PEP-009) is available for use in neutralization and control experiments.

Target Information

Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G- protein that activates a phosphatidylinositol-calcium second messenger system.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Calcium-Sensing Receptor Contributes to Hyperoxia Effects on Human Fetal Airway Smooth Muscle.

Frontiers in physiology

Roesler AM,Ravix J,Bartman CM,Patel BS,Schiliro M,Roos B,Nesbitt L,Pabelick CM,Martin RJ,MacFarlane PM,Prakash YS

Published figure using Calcium Sensing Receptor polyclonal antibody (Product # PA1-934A) in Immunocytochemistry

Tue May 11 00:00:00 EDT 2021

Calcium sensing receptor in developing human airway smooth muscle.

Journal of cellular physiology

Roesler AM,Wicher SA,Ravix J,Britt RD,Manlove L,Teske JJ,Cummings K,Thompson MA,Farver C,MacFarlane P,Pabelick CM,Prakash YS

PA1-934A was used in Immunocytochemistry-immunoflourescence to demonstrate functional Ca2+ ([Ca2+ ]o ) sensing receptor (CaSR) in developing ASM that modulates airway contractility and proliferation.

Thu Aug 01 00:00:00 EDT 2019

Evidence for a regulated Ca²⁺ entry in proximal tubular cells and its implication in calcium stone formation.

Journal of cell science

Ibeh CL,Yiu AJ,Kanaras YL,Paal E,Birnbaumer L,Jose PA,Bandyopadhyay BC

PA1-934A was used in Immunohistochemistry to show the existence of a regulated transcellular Ca2+ entry pathway in luminal membrane proximal tubule cells induced by Ca2+ sensing receptor-mediated activation of transient receptor potential canonical 3 channels.

Tue Apr 30 00:00:00 EDT 2019

Evidence for a regulated Ca²⁺ entry in proximal tubular cells and its implication in calcium stone formation.

Journal of cell science

Ibeh CL,Yiu AJ,Kanaras YL,Paal E,Birnbaumer L,Jose PA,Bandyopadhyay BC

Tue Apr 30 00:00:00 EDT 2019

Correlation between Choline Peak at MR Spectroscopy and Calcium-Sensing Receptor Expression Level in Breast Cancer: A Preliminary Clinical Study.

Molecular imaging and biology

Baio G,Rescinito G,Rosa F,Pace D,Boccardo S,Basso L,Salvi S,Calabrese M,Truini M,Neumaier CE

PA1-934A was used in immunohistochemistry - paraffin section to correlate choline with the CaSR expression surgical breast specimens

Sat Aug 01 00:00:00 EDT 2015

Decreased parathyroid Klotho expression is associated with persistent hyperparathyroidism after kidney transplantation.

Transplantation proceedings

Hong YA,Choi DE,Lim SW,Yang CW,Chang YK

PA1-934A was used in immunohistochemistry to study persistent hypoparathyroidism in kidney transplant patients and the role of reduced parathyroid expression of Klotho

Tue Oct 01 00:00:00 EDT 2013

A novel germline inactivating mutation in the CASR gene in an Italian kindred affected by familial hypocalciuric hypercalcemia.

European journal of endocrinology

Falchetti A,Gozzini A,Terranegra A,Soldati L,Vezzoli G,Leoncini G,Giusti F,Franceschelli F,Masi L,Tanini A,Cavalli L,Brandi ML

PA1-934A was used in western blot to study a novel germline CASR mutation in a case of familial hypocaliuric hypercalcemia in an Italian family

Tue May 01 00:00:00 EDT 2012

In vivo imaging of human breast cancer mouse model with high level expression of calcium sensing receptor at 3T.

European radiology

Baio G,Fabbi M,Emionite L,Cilli M,Salvi S,Ghedin P,Prato S,Carbotti G,Tagliafico A,Truini M,Neumaier CE

PA1-934A was used in immunohistochemistry to evaluate an in vivo breast cancer cell imaging technique

Thu Mar 01 00:00:00 EST 2012

Establishment and characterization of a novel cell line derived from a human small cell lung carcinoma that secretes parathyroid hormone, parathyroid hormone-related protein, and pro-opiomelanocortin.

Human cell

Ishikawa M,Kimura K,Tachibana T,Hashimoto H,Shimojo M,Ueshiba H,Tsuboi K,Shibuya K,Yoshino G

PA1-934A was used in immunohistochemistry to characterize a novel hormone-secreting cell line derived from human small cell lung carcinoma

Sat May 01 00:00:00 EDT 2010

Regulation of mouse lung development by the extracellular calcium-sensing receptor, CaR.

The Journal of physiology

Finney BA,del Moral PM,Wilkinson WJ,Cayzac S,Cole M,Warburton D,Kemp PJ,Riccardi D

PA1-934A was used in immunohistochemistry to investigate the role of calcium-sensing receptor in mouse lung development

Mon Dec 15 00:00:00 EST 2008

Normocalcemic primary hyperparathyroidism in patients with recurrent kidney stones: pathological analysis of parathyroid glands.

Virchows Archiv : an international journal of pathology

Yang AH,Hsu CW,Chen JY,Tseng LM,Won GS,Lee CH

PA1-934A was used in immunohistochemistry to study the abnormalities in parathyroid tissues from patients with normocalcemic primary hyperparathyroidism

Sat Jul 01 00:00:00 EDT 2006

The calcium sensing receptor and its alternatively spliced form in keratinocyte differentiation.

The Journal of biological chemistry

Oda Y,Tu CL,Pillai S,Bikle DD

PA1-934A was used in western blot to study the functional properties of the calcium-sensing receptor and its alternatively spliced form in keratinocyte differentiation

Fri Sep 04 00:00:00 EDT 1998

Bioinformatics

Protein Aliases: Ca sensing receptor; calcium-sensing receptor; Calcium-sensing receptor (hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism); Calcium-sensing receptor (hypocalciuric hypercalcemia 1, severe neonatal hyperparathyroidism); CaR; CaSR; CaSR antibody; Extracellular calcium-sensing receptor; parathyroid Ca(2+)-sensing receptor 1; Parathyroid cell calcium-sensing receptor; Parathyroid cell calcium-sensing receptor 1; PCaR1

Gene Aliases: CAR; CASR; EIG8; FHH; FIH; GPRC2A; HHC; HHC1; HYPOC1; NSHPT; PCAR1

UniProt ID: (Human) P41180, (Rat) P48442

Entrez Gene ID: (Human) 846, (Rat) 24247

Molecular Function: G-protein coupled receptor