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Calcyclin Antibody (720326) in WB
Western blot analysis was performed on Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), Jurkat (Lane 3), A549 (Lane 4), HEK-293 (Lane 5) and MCF-7 (Lane 6). The blots were probed with Anti-Calcyclin binding protein Rabbit Polyclonal Antibody (Product # 720326, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 30 kDa band corresponding to Calcyclin binding protein was observed across the cell lines... View More
Product Details
720326
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Peptides corresponding to Human CACYBP (aa 47-60, 155-168)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 30% glycerol, 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2633259
Product Specific Information
These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
This antibody is predicted to react with Monkey, Mouse, Rat and Bovine
Target Information
Calcyclin is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in stimulation of Ca2+-dependent insulin release, stimulation of prolactin secretion, and exocytosis. Chromosomal rearrangements and altered expression of this gene have been implicated in melanoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 2A9; CACY; CacyBP; Calcyclin-binding protein; growth-inhibiting gene 5 protein; hCacyBP; RP1-102G20.6; S100A6; S100A6-binding protein; Siah-interacting protein; Siah-interacting protein (SIP)
Gene Aliases: CACYBP; GIG5; PNAS-107; S100A6BP; SIP
UniProt ID: (Human) Q9HB71
Entrez Gene ID: (Human) 27101
Molecular Function:
ubiquitin-protein ligase
protein modifying enzyme
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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