Invitrogen

Calmodulin 2 Polyclonal Antibody

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Calmodulin 2 Antibody in Western Blot (WB) — Western blot analysis of Calmodulin 2 using 50 µg of mouse brain lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with a Calmodulin 2 polyclonal antibody (Product # PA5-27515) at a dilution of 1:1000.

Calmodulin 2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of Calmodulin 2 was performed in DIV9 rat E18 primary cortical neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Calmodulin 2 Polyclonal Antibody (Product # PA5 27515) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3000

-

Immunocytochemistry (ICC/IF)

1:100-1:1000

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 165 of Human Calmodulin 2

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2544991

Product Specific Information

Recommended positive controls: Mouse brain.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (100%), Rabbit (100%), Sheep (100%), Chimpanzee (100%), Bovine (100%), Guinea pig (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: calmodulin; calmodulin 2 (phosphorylase kinase, delta); Calmodulin-1; Calmodulin-2; Calmodulin-3; caM; LP7057 protein; phosphorylase kinase delta; phosphorylase kinase subunit delta; prepro-calmodulin 2

Gene Aliases: 1500001E21Rik; AL024017; CALM; CALM1; CALM2; CALM3; CALML2; CAM; CAM1; CAM2; CAM3; CAMB; CAMC; CaMI; CAMII; CAMIII; LQT15; PHKD; PHKD2

UniProt ID: (Human) P0DP24, (Rat) P0DP30, (Mouse) P0DP27

Entrez Gene ID: (Human) 805, (Rat) 50663, (Mouse) 12314

Molecular Function: calcium-binding protein calmodulin intracellular calcium-sensing protein

G2/M transition of mitotic cell cycle MAPK cascade response to amphetamine regulation of heart rate platelet degranulation detection of calcium ion glycogen catabolic process muscle contraction G-protein coupled receptor signaling pathway activation of adenylate cyclase activity Wnt signaling pathway, calcium modulating pathway positive regulation of peptidyl-threonine phosphorylation negative regulation of peptidyl-threonine phosphorylation regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion substantia nigra development regulation of rhodopsin mediated signaling pathway positive regulation of cyclic nucleotide metabolic process positive regulation of protein autophosphorylation regulation of cytokinesis positive regulation of phosphoprotein phosphatase activity ion transmembrane transport positive regulation of protein dephosphorylation Fc-epsilon receptor signaling pathway positive regulation of DNA binding positive regulation of GTPase activity inositol phosphate metabolic process regulation of nitric-oxide synthase activity positive regulation of nitric-oxide synthase activity positive regulation of cyclic-nucleotide phosphodiesterase activity response to corticosterone response to calcium ion regulation of cardiac muscle contraction negative regulation of ryanodine-sensitive calcium-release channel activity positive regulation of ryanodine-sensitive calcium-release channel activity positive regulation of protein serine/threonine kinase activity regulation of high voltage-gated calcium channel activity regulation of cell communication by electrical coupling involved in cardiac conduction regulation of catalytic activity

Process(es)

Ras guanyl-nucleotide exchange factor activity calcium ion binding protein binding adenylate cyclase binding inositol-1,4,5-trisphosphate 3-kinase activity ligand-gated ion channel activity protein kinase binding protein domain specific binding nitric-oxide synthase regulator activity titin binding type 3 metabotropic glutamate receptor binding thioesterase binding N-terminal myristoylation domain binding phospholipase binding protein serine/threonine kinase activator activity phosphatidylinositol 3-kinase binding ion channel binding calcium-dependent protein binding nitric-oxide synthase binding protein phosphatase activator activity enzyme regulator activity protein N-terminus binding