Invitrogen

Calmodulin 3 Polyclonal Antibody

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Calmodulin 3 Antibody in Western Blot (WB) — Western blot analysis of extracts of rat testis, using Calmodulin 3 Polyclonal antibody (Product # PA5-89886) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s.

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:50-1:100

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CALM3

Conjugate

Unconjugated

Form

liquid

Concentration

0.87 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen sequence: MADQLTEEQI AEFKEAFSLF RVRVLQGVRL SENVVNRMKE PSSPPPAPTS STFGLQDGNL RAPHKESTLP RSGSSGGQQP SGMKEGVKRY EQEHAAIQDK LFQVAKRERE AATKHSKASL PTGEGSISHE EQKSVRLARE LESREAELRR RDTFYKEQLE RIERKNAEMY KLSSEQFHEA ASKMESTIKP RRVEPVCSGL QAQILHCYRD RPHEVLLCSD LVKAYQRCVS AAHKG; Positive Samples: Rat testis; Cellular Location: Cytoplasm, cytoskeleton, spindle, spindle pole

Target Information

Calcium binding proteins (CaBP) play a crucial role in the calcium-mediated cellular signal transduction pathway in the central nervous system. The CaBP family shares much similarity with CaM I (calmodulin), and it has been shown that CaBP proteins can substitute functionally for, and possibly augment the function of, CaM I. CaBP7 (Calcium-binding protein 7), contains two EF-hand domains for calcium binding and shares 70% homology with CaBP8 and 50% homology with CaM I. It negatively regulates Golgi-to-plasma membrane trafficking by interacting with PI4KB and inhibiting its activity. CaBP7 and 8 possess a targeting mechanism that is unique amongst the CaBPs that may contribute to differential functional Ca2+-sensing by these family members.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.