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Invitrogen
Calmodulin Monoclonal Antibody (6D4)
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FIGURE: 1 / 6
Calmodulin Antibody (MA3-918) in ICC/IF
Immunofluorescence analysis of Calmodulin was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Calmodulin (6D4) Mouse Monoclonal Antibody (Product # MA3-918) at 1:250 dilution in 0.1% BSA and incubated for 3 hours a... View More
Product Details
MA3-918
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria,
Bovine,
Chicken,
Human,
Rat
Published species
Algae,
Cnidarian,
Coral,
Fruit fly,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
6D4
Immunogen
Calmodulin purified from Dictyostelium discoideum.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-918 detects calmodulin from bovine, chicken, Chlamydomonas, Dictyostelium, and rat samples. This antibody does not detect parvalbumin, tropinin, S-100, or myosin light chain kinase (MLCK).
MA3-918 has been successfully used in Western blot, immunocytochemistry and ELISA procedures. By Western blot, this antibody detects a 17 kDa protein representing calmodulin from Dictyostelium cell lysate. Addition of EGTA to buffers completely inhibits antibody staining. Immunohistochemical staining of calmodulin in Dictyostelium cells with MA3-918 results in staining of the contractile vacuoles.
The MA3-918 antigen is calmodulin purified from Dictyostelium discoideum.
Target Information
Calmodulin mediates the control of a large number of enzymes, ion channels, aquaporins and other proteins by Ca2+. Among the enzymes to be stimulated by the calmodulin-Ca2+ complex are a number of protein kinases and phosphatases. Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis. Calmodulin is a small, highly conserved calcium binding protein found in all eukaryotic cells. With the capacity to bind up to four calcium ions, this 17 kDa protein acts as an important intracellular receptor for regulatory calcium signals. As it binds calcium, calmodulin undergoes conformational changes which can increase its affinity for target proteins. It acts both directly, through interaction with key target enzymes, and indirectly, via specific kinases. Studies have found that calmodulin participates in the regulation of several biological processes including energy and biosynthetic metabolism, cell motility, exocytosis, cytoskeletal assembly, and intracellular modulation of both cAMP and calcium concentrations. Calmodulin is the archetype of the family of calcium-modulated proteins of which nearly 20 members have been found. They are identified by their occurrence in the cytosol or on membranes facing the cytosol and by a high affinity for calcium. Calmodulin contains 149 amino acids and has 4 calcium-binding domains. Its functions include roles in growth and the cell cycle as well as in signal transduction and the synthesis and release of neurotransmitters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CALM 1; CALM2; Calmodulin; Calmodulin 1 (phosphorylase kinase, delta); calmodulin 2 (phosphorylase kinase, delta); Calmodulin III; Calmodulin-1; Calmodulin-2; Calmodulin-3; calmodulin-like protein; calmodulin-like protein (neoCaM); CaM; CAMI; DD312; epididymis secretory protein Li 72; LP7057 protein; Neo-calmodulin; NeoCaM; phosphorylase kinase delta; phosphorylase kinase subunit delta; phosphorylase kinase subunit delta 1; phosphorylase kinase subunit delta 2; phosphorylase kinase subunit delta 3; phosphorylase kinase, delta subunit; prepro-calmodulin 1; prepro-calmodulin 2; prepro-calmodulin 3; unnamed protein product
Gene Aliases: CALM; CALM1; CALM2; CALM3; CALML2; CAM; CAM1; CAM2; CAM3; CAMB; CAMC; CAMI; CAMII; CAMIII; CPVT4; CPVT6; DD132; HEL-S-72; LQT14; LQT15; LQT16; PHKD; PHKD1; PHKD2; PHKD3; RCJMB04_24e7; RNCAMIII
UniProt ID: (Chicken) P05419, (Human) P0DP23, (Chicken) P62149, (Rat) P0DP29, (Rat) P0DP31
calcium ion binding
protein binding
protein domain specific binding
enzyme regulator activity
calcium channel regulator activity
adenylate cyclase activator activity
calcium channel inhibitor activity
protein kinase binding
titin binding
protein serine/threonine kinase activator activity
ion channel binding
metal ion binding
calcium-dependent protein binding
protein phosphatase activator activity
transporter inhibitor activity
adenylate cyclase binding
protein C-terminus binding
myosin binding
CH domain binding
disordered domain specific binding
nitric-oxide synthase regulator activity
type 3 metabotropic glutamate receptor binding
phosphatidylinositol 3-kinase binding
nitric-oxide synthase binding
thioesterase binding
N-terminal myristoylation domain binding
phospholipase binding
protein N-terminus binding
calmodulin-related
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
regulation of catalytic activity
negative regulation of ryanodine-sensitive calcium-release channel activity
positive regulation of ryanodine-sensitive calcium-release channel activity
microtubule cytoskeleton organization
calcium-mediated signaling
G2/M transition of mitotic cell cycle
regulation of heart rate
detection of calcium ion
G-protein coupled receptor signaling pathway
JAK-STAT cascade
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
autophagosome membrane docking
substantia nigra development
regulation of cytokinesis
cellular response to interferon-beta
positive regulation of JAK-STAT cascade
regulation of calcium-mediated signaling
response to calcium ion
regulation of cardiac muscle contraction
long-term synaptic potentiation
regulation of ryanodine-sensitive calcium-release channel activity
cellular response to interferon-gamma
calcineurin-mediated signaling
regulation of cardiac muscle cell action potential
organelle localization by membrane tethering
presynaptic endocytosis
negative regulation of high voltage-gated calcium channel activity
regulation of cell communication by electrical coupling involved in cardiac conduction
negative regulation of calcium ion export across plasma membrane
mitochondrion-ER tethering
response to amphetamine
response to corticosterone
establishment of protein localization to membrane
establishment of protein localization to mitochondrial membrane
regulation of synaptic vesicle endocytosis
regulation of synaptic vesicle exocytosis
activation of adenylate cyclase activity
positive regulation of cyclic nucleotide metabolic process
positive regulation of phosphoprotein phosphatase activity
positive regulation of protein dephosphorylation
positive regulation of DNA binding
positive regulation of nitric-oxide synthase activity
positive regulation of cyclic-nucleotide phosphodiesterase activity
regulation of store-operated calcium channel activity
regulation of high voltage-gated calcium channel activity
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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