Proteintech

Calpain 2 Polyclonal Antibody

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Calpain 2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using CAPN2 antibody 11472-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Calpain 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using 11472-1-AP (Calpain 2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Calpain 2 Antibody in Western Blot (WB) — Human placenta tissue were subjected to SDS PAGE followed by western blot with 11472-1-AP (Calpain 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

11472-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Calpain 2 Fusion Protein Ag2029 (1-330 aa encoded by BC021303)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MAGIAAKLA KDREAAEGLG SHERAIKYLN QDYEALRNEC LEAGTLFQDP SFPAIPSALG FKELGPYSSK TRGIEWKRPT EICADPQFII GGATRTDICQ GALGDCWLLA AIASLTLNEE ILARVVPLNQ SFQENYAGIF HFQFWQYGEW VEVVVDDRLP TKDGELLFVH SAEGSEFWSA LLEKAYAKIN GCYEALSGGA TTEGFEDFTG GIAEWYELKK PPPNLFKIIQ KALQKGSLLG CSIDITSAAD SEAITFQKLV KGHAYSVTGA EEVESNGSLQ KLIRIRNPWG EVEWTGRWND NCPSWNTIDP EERERLTRRH EDGEFWMSFS D (1-330 aa encoded by BC021303)

Target Information

Mu-Calpain, also known as Calpain-I, and m-calpain, also known as Calpain-II, are intracellular, calcium-dependent cysteine proteases. Mu-calpain has a micromolar sensitivity (thus the mu-) as compared to the millimolar calcium sensitivity of m-calpain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis.

Journal of translational medicine

Liu X,Jiang L,Zhang W,Zhang J,Luan X,Zhan Y,Wang T,Da J,Liu L,Zhang S,Guo Y,Zhang K,Wang Z,Miao N,Xie X,Liu P,Li Y,Jin H,Zhang B

Tue Jun 27 00:00:00 EDT 2023

Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis.

Journal of translational medicine

Liu X,Jiang L,Zhang W,Zhang J,Luan X,Zhan Y,Wang T,Da J,Liu L,Zhang S,Guo Y,Zhang K,Wang Z,Miao N,Xie X,Liu P,Li Y,Jin H,Zhang B

Tue Jun 27 00:00:00 EDT 2023

Fam20c regulates the calpain proteolysis system through phosphorylating Calpasatatin to maintain cell homeostasis.

Journal of translational medicine

Liu X,Jiang L,Zhang W,Zhang J,Luan X,Zhan Y,Wang T,Da J,Liu L,Zhang S,Guo Y,Zhang K,Wang Z,Miao N,Xie X,Liu P,Li Y,Jin H,Zhang B

Tue Jun 27 00:00:00 EDT 2023

Calpain inhibitor MDL28170 alleviates cerebral ischemia‑reperfusion injury by suppressing inflammation and autophagy in a rat model of cardiac arrest.

Experimental and therapeutic medicine

Wang WY,Shi JX,Chen MH,Zhuge XZ,Dai CG,Xie L

Mon May 01 00:00:00 EDT 2023

Calpain inhibitor MDL28170 alleviates cerebral ischemia‑reperfusion injury by suppressing inflammation and autophagy in a rat model of cardiac arrest.

Experimental and therapeutic medicine

Wang WY,Shi JX,Chen MH,Zhuge XZ,Dai CG,Xie L

Mon May 01 00:00:00 EDT 2023

Cardiac Myocyte-Specific Overexpression of FASTKD1 Prevents Ventricular Rupture After Myocardial Infarction.

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Marshall KD,Klutho PJ,Song L,Roy R,Krenz M,Baines CP

Tue Feb 21 00:00:00 EST 2023

Prominent Effects of Berbamine Hydrochloride on Alzheimer's Disease Model Mice.

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Chen CL,Wang HL,Qian F,Liu MH,Guo XX,Lv J,Huang JH,Zhang NZ,Xiang Z

Tue Jul 19 00:00:00 EDT 2022

TRPC1 contributes to endotoxemia-induced myocardial dysfunction via mediating myocardial apoptosis and autophagy.

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Tian W,Liu SY,Zhang M,Meng JR,Tang N,Feng YD,Sun Y,Gao YY,Zhou L,Cao W,Li XQ

Fri Jul 01 00:00:00 EDT 2022

Mechanosensitive Piezo1 channels mediate renal fibrosis.

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Utility of 7,8-dihydroxyflavone in preventing astrocytic and synaptic deficits in the hippocampus elicited by PTSD.

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Wang J,Gao F,Cui S,Yang S,Gao F,Wang X,Zhu G

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Astrocytic Kir4.1 regulates NMDAR/calpain signaling axis in lipopolysaccharide-induced depression-like behaviors in mice.

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Song Z,Bian Z,Zhang Z,Wang X,Zhu A,Zhu G

Fri Oct 15 00:00:00 EDT 2021

The effect of repeated bouts of electrical stimulation-induced muscle contractions on proteolytic signaling in rat skeletal muscle.

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Inhibition of Shear-Induced Platelet Aggregation by Xueshuantong via Targeting Piezo1 Channel-Mediated Ca²⁺ Signaling Pathway.

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Liu L,Zhang Q,Xiao S,Sun Z,Ding S,Chen Y,Wang L,Yin X,Liao F,Jiang LH,Xue M,You Y

Tue Apr 13 00:00:00 EDT 2021

Inhibition of extracellular signal-regulated kinase/calpain-2 pathway reduces neuroinflammation and necroptosis after cerebral ischemia-reperfusion injury in a rat model of cardiac arrest.

International immunopharmacology

Wang WY,Xie L,Zou XS,Li N,Yang YG,Wu ZJ,Tian XY,Zhao GY,Chen MH

Thu Apr 01 00:00:00 EDT 2021

Inhibition of extracellular signal-regulated kinase/calpain-2 pathway reduces neuroinflammation and necroptosis after cerebral ischemia-reperfusion injury in a rat model of cardiac arrest.

International immunopharmacology

Wang WY,Xie L,Zou XS,Li N,Yang YG,Wu ZJ,Tian XY,Zhao GY,Chen MH

Thu Apr 01 00:00:00 EDT 2021

Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

Wang S,Huang Y,Yan Y,Zhou H,Wang M,Liao L,Wang Z,Chen D,Ji D,Xia X,Liu F,Huang J,Xiong K

Tue Jan 01 00:00:00 EST 2019

Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

Wang S,Huang Y,Yan Y,Zhou H,Wang M,Liao L,Wang Z,Chen D,Ji D,Xia X,Liu F,Huang J,Xiong K

Tue Jan 01 00:00:00 EST 2019

Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

Wang S,Huang Y,Yan Y,Zhou H,Wang M,Liao L,Wang Z,Chen D,Ji D,Xia X,Liu F,Huang J,Xiong K

Tue Jan 01 00:00:00 EST 2019

Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons.

Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft

Wang S,Huang Y,Yan Y,Zhou H,Wang M,Liao L,Wang Z,Chen D,Ji D,Xia X,Liu F,Huang J,Xiong K

Tue Jan 01 00:00:00 EST 2019

Bioinformatics

Protein Aliases: 80 kDa M-calpain subunit; Calcium-activated neutral proteinase 2; CALP80; calpain 2, (m/II) large subunit; calpain 2, large [catalytic] subunit; calpain 2, large subunit; Calpain large polypeptide L2; Calpain M-type; calpain, large polypeptide L2; Calpain-2 catalytic subunit; Calpain-2 large subunit; CANP 2; cysteine proteinase; M-calpain; m-calpain 80 kDa large subunit; m-calpain large subunit; Millimolar-calpain

Gene Aliases: AI326419; CANP2; CANPL2; CANPml; Capa-2; Capa2; CAPN2; m-calpain; m-calpin; mCANP

UniProt ID: (Human) P17655, (Mouse) O08529, (Rat) Q07009

Entrez Gene ID: (Human) 824, (Mouse) 12334, (Rat) 29154

Molecular Function: cysteine protease