|Tested species reactivity||Human, Mouse, Rabbit, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Purified rabbit skeletal muscle calreticulin|
|Storage buffer||whole serum diluted in glycine buffered saline, pH 7.4|
|Contains||0.09% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:50-1:70|
|Western Blot (WB)||1:300-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-33045 detects Calreticulin from human, mouse, rat, rabbit samples.
PA1-33045 has been successfully used in immunohistochemistry (frozen tissue) and Western blot applications. This antibody is not suitable for imunoprecipitation.
The PA1-33045 immunogen is purified rabbit skeletal muscle calreticulin.
Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. This protein was originally identified in SR membranes and plays a minor role in calcium storage in skeletal and cardiac muscle SR. Calreticulin is also known as calregulin, CRP55, CaBP3, calsequestrin-like protein and Ro/SS-A antigen. Calreticulin binds calcium with low affinity and high capacity, however it also exhibits a single high affinity binding site. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of calreticulin and other resident ER proteins including glucose regulated protein 78 (GRP78), GRP94 and protein disulfide isomerase (PDI). This sequence is responsible for the retention of newly synthesized proteins within the ER lumen. This retention is reported to be mediated by a KDEL receptor. Recent reports indicate that calreticulin can act as a modulator of the regulation of gene transcription by nuclear hormone receptors and may also act as a molecular chaperone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.