Western blot analysis was performed on membrane enriched extracts (30 ug lysate) of A-549 (Lane 1), HEK 293 (Lane 2) and Mouse Brain (Lane 3). The blot was probed with Anti-Calreticulin Chicken Polyclonal Antibody (Product # PA1-902A, 1:500-1:2000 dilution) and detected by chemiluminescence using Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate (Product # A16054, 0.4 ug/ml, 1:2500 dilution). A 48 kDa band corresponding to Calreticulin was observed across the cell lines and tissue tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
|Tested species reactivity||Dog, Hamster, Human, Mouse, Rat|
|Published species reactivity||Human, Mouse, Not Applicable|
|Host / Isotype||Chicken / IgY|
|Immunogen||Synthetic Peptide: K(24) E Q F L D G D A W T N R W V E S K H K(43).|
|Purification||Antigen affinity chromatography|
|Storage buffer||1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Blocking Assay (BLOCK)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
PA1-902A detects calreticulin from canine, hamster, human, mouse and rat tissues.
PA1-902A has been successfully used in Western blot, immunocytochemistry and immunofluorescene procedures. By Western blot, this antibody detects an ~60-66 kDa protein representing calreticulin.
PA1-902A immunizing peptide corresponds to amino acid residues 24-43 from mouse calreticulin. PA1-902A immunizing peptide (Cat. # PEP-018) is available for use in neutralization and control experiments.
Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. This protein was originally identified in SR membranes and plays a minor role in calcium storage in skeletal and cardiac muscle SR. Calreticulin is also known as calregulin, CRP55, CaBP3, calsequestrin-like protein and Ro/SS-A antigen. Calreticulin binds calcium with low affinity and high capacity, however it also exhibits a single high affinity binding site. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of calreticulin and other resident ER proteins including glucose regulated protein 78 (GRP78), GRP94 and protein disulfide isomerase (PDI). This sequence is responsible for the retention of newly synthesized proteins within the ER lumen. This retention is reported to be mediated by a KDEL receptor. Recent reports indicate that calreticulin can act as a modulator of the regulation of gene transcription by nuclear hormone receptors and may also act as a molecular chaperone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Concurrent MEK and autophagy inhibition is required to restore cell death associated danger-signalling in Vemurafenib-resistant melanoma cells.
PA1-902A was used in blocking or activating experiment to study the role of autophagy in melanoma
|Martin S,Dudek-Peri¿ AM,Maes H,Garg AD,Gabrysiak M,Demirsoy S,Swinnen JV,Agostinis P||Biochemical pharmacology (93:290)||2015|
Telocytes subtypes in human urinary bladder.
PA1-902A was used in immunohistochemistry - paraffin section to describe human urinary bladder by phenotypically by transmission electron microscopy (TEM) and immunohistochemistry
|Vannucchi MG,Traini C,Guasti D,Del Popolo G,Faussone-Pellegrini MS||Journal of cellular and molecular medicine (18:2000)||2014|
Inner and outer portions of colonic circular muscle: ultrastructural and immunohistochemical changes in rat chronically treated with otilonium bromide.
PA1-902A was used in immunohistochemistry - frozen section and western blot to study the ultrastructural and immunohistochemical changes of inner and outer portions of colonic circular muscle in rats treated with otilonium bromide chronically
|Traini C,Faussone-Pellegrini MS,Evangelista S,Mazzaferro K,Cipriani G,Santicioli P,Vannucchi MG||PloS one (9:null)||2014|
Relative contribution of c1q and apoptotic cell-surface calreticulin to macrophage phagocytosis.
PA1-902A was used in immunocytochemistry to study the role in macrophage phagocytosis of the interaction between c1q and calreticulin
|Verneret M,Tacnet-Delorme P,Osman R,Awad R,Grichine A,Kleman JP,Frachet P||Journal of innate immunity (6:426)||2014|
Cytoplasmic domain of herpes simplex virus gE causes accumulation in the trans-Golgi network, a site of virus envelopment and sorting of virions to cell junctions.
PA1-902A was used in immunocytochemistry to investigate the role of the CT domain of HSV gE in the trans-Golgi network.
|McMillan TN,Johnson DC||Journal of virology (75:1928)||2001|
Endoplasmic reticulum calcium depletion impacts chaperone secretion, innate immunity, and phagocytic uptake of cells.
PA1-902A was used in flow cytometry to investigate the effect of endoplasmic reticulum calcium depletion on cellular function
|Peters LR,Raghavan M||Journal of immunology (Baltimore, Md. : 1950) (187:919)||2011|
Chronic ethanol consumption in mice alters hepatocyte lipid droplet properties.
PA1-902A was used in immunohistochemistry to study the effects of chronic ethanol consumption on hepatocyte lipid droplets in mice
|Orlicky DJ,Roede JR,Bales E,Greenwood C,Greenberg A,Petersen D,McManaman JL||Alcoholism, clinical and experimental research (35:1020)||2011|
The WFS1 gene, responsible for low frequency sensorineural hearing loss and Wolfram syndrome, is expressed in a variety of inner ear cells.
PA1-902A was used in immunohistochemistry to study the expression of wolframin in mouse inner ear at different developmental stages
|Cryns K,Thys S,Van Laer L,Oka Y,Pfister M,Van Nassauw L,Smith RJ,Timmermans JP,Van Camp G||Histochemistry and cell biology (119:247)||2003|