|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:2,000|
|Immunohistochemistry (IHC)||See 1 publications below|
|Western Blot (WB)||See 8 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Dog, Hamster, Human, Mouse, Rabbit, Rat|
|Published species reactivity||Rabbit , Rat , Amphibian , Human Mouse|
|Host / Isotype||Chicken / IgY|
|Immunogen||Synthetic Peptide: D(399) E K E E D E E E S P G Q A K D(414).|
|Purification||Antigen affinity chromatography|
|Storage buffer||1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-903 detects calreticulin from human, rat, mouse, hamster, rabbit and canine tissues.
PA1-903 has been successfully used in Western blot, immunoprecipitation, immunocytochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa protein representing calreticulin and an ~120 kDa unidentified protein from rat brain extract.
PA1-903 immunizing peptide corresponds to amino acid residues 399-414 from mouse calreticulin. PA1-903 immunizing peptide (Cat. # PEP-017) is available for use in neutralization and control experiments.
Calreticulin is the major calcium binding protein found in smooth muscle sarcoplasmic reticulum (SR) and non-muscle endoplasmic reticulum (ER) membranes. This protein was originally identified in SR membranes and plays a minor role in calcium storage in skeletal and cardiac muscle SR. Calreticulin is also known as calregulin, CRP55, CaBP3, calsequestrin-like protein and Ro/SS-A antigen. Calreticulin binds calcium with low affinity and high capacity, however it also exhibits a single high affinity binding site. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C-terminus of calreticulin and other resident ER proteins including glucose regulated protein 78 (GRP78), GRP94 and protein disulfide isomerase (PDI). This sequence is responsible for the retention of newly synthesized proteins within the ER lumen. This retention is reported to be mediated by a KDEL receptor. Recent reports indicate that calreticulin can act as a modulator of the regulation of gene transcription by nuclear hormone receptors and may also act as a molecular chaperone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CABP3; CALBP; Calcium-binding protein 3; Calregulin; Calreticulin; Calsequestrin-Like Protein; CRP55; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; grp60; HACBP; Ro/SS-A Antigen; Sicca syndrome antigen A (autoantigen Ro; calreticulin)
Gene Aliases: CALR; Calregulin; cC1qR; CRT; CRTC; HEL-S-99n; RO; SSA
Molecular Function: calcium-binding protein
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.