Proteintech

Calsequestrin 2 Polyclonal Antibody

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Calsequestrin 2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed C2C12 cell using 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Calsequestrin 2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart tissue slide using 18422-1-AP ( Calsequestrin 2 antibody at dilution of 1:200 (under 40x lens).

Calsequestrin 2 Antibody in Western Blot (WB) — Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18422-1-AP (Calsequestrin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

18422-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Pig, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Calsequestrin 2 Fusion Protein Ag13246 (1-399 aa encoded by BC022288) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.26 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MKRTHLFIV GIYFLSSCRA EEGLNFPTYD GKDRVVSLSE KNFKQVLKKY DLLCLYYHEP VSSDKVTQKQ FQLKEIVLEL VAQVLEHKAI GFVMVDAKKE AKLAKKLGFD EEGSLYILKG DRTIEFDGEF AADVLVEFLL DLIEDPVEII SSKLEVQAFE RIEDYIKLIG FFKSEDSEYY KAFEEAAEHF QPYIKFFATF DKGVAKKLSL KMNEVDFYEP FMDEPIAIPN KPYTEEELVE FVKEHQRPTL RRLRPEEMFE TWEDDLNGIH IVAFAEKSDP DGYEFLEILK QVARDNTDNP DLSILWIDPD DFPLLVAYWE KTFKIDLFRP QIGVVNVTDA DSVWMEIPDD DDLPTAEELE DWIEDVLSGK INTEDDDEDD DDDDNSDEED NDDSDDDDDE (1-399 aa encoded by BC022288)

Target Information

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Fer1l6 is essential for the development of vertebrate muscle tissue in zebrafish.

Molecular biology of the cell

Bonventre JA,Holman C,Manchanda A,Codding SJ,Chau T,Huegel J,Barton C,Tanguay R,Johnson CP

Fri Feb 01 00:00:00 EST 2019

SK4 K⁺ channels are therapeutic targets for the treatment of cardiac arrhythmias.

EMBO molecular medicine

Haron-Khun S,Weisbrod D,Bueno H,Yadin D,Behar J,Peretz A,Binah O,Hochhauser E,Eldar M,Yaniv Y,Arad M,Attali B

Sat Apr 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: calsequestrin 2 (cardiac muscle); calsequestrin 2, fast-twitch, cardiac muscle; Calsequestrin, cardiac muscle isoform; Calsequestrin-2; cardiac calsequestrin; SR calcium binding protein

Gene Aliases: AA033488; AW146219; cardCSQ; CASQ2; cCSQ; Csq2; ESTM52; PDIB2

UniProt ID: (Human) O14958, (Mouse) O09161, (Rat) P51868

Entrez Gene ID: (Human) 845, (Pig) 397436, (Mouse) 12373, (Rat) 29209

Molecular Function: calcium-binding protein