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Invitrogen
Calsequestrin Polyclonal Antibody
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FIGURE: 1 / 84
Calsequestrin Antibody (PA1-913) in ICC/IF
Immunofluorescent analysis of Calsequestrin (green) showing staining in the cytoplasm and nucleus of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Calsequestrin polyclonal antibody (Product # PA1-... View More
Product Details
PA1-913
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Sheep,
Rat
Published species
Cat,
Dog,
Human,
Mouse,
Rabbit,
Rat,
Sheep,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified canine cardiac calsequestrin.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum, PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-913 detects calsequestrin from canine, human, mouse, rat and sheep tissues. This antibody recognizes both cardiac and skeletal muscle calsequestrin.
PA1-913 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, immunohistochemistry (paraffin) and immunoprecipitation procedures. By Western blot, this antibody detects an ~55 kDa protein representing calsequestrin from canine cardiac extract. Additional bands at 97 kDa may be observed and have been reported to be calsequestrin-like proteins.
PA1-913 antigen is purified canine cardiac calsequestrin.
Target Information
The sarcoplasmic reticulum (SR) is, in part, responsible for maintaining the level of intracellular calcium in cardiac and skeletal muscle by storing and releasing calcium. Several intralumenal SR calcium binding proteins have been identified, the most prominent of these is calsequestrin. Calsequstrin is a calcium binding protein known to sequester calcium accumulated in the sarcoplasmic reticulum of muscle cells during relaxation and is found discretely localized to the junctional and corbular (terminal cisternae) SR. Calsequestrin functions to localize calcium near the junctional face of the terminal cisternae from which calcium can be released into the cytosol via the ryanodine receptor. This protein is highly acidic and has a large capacity and moderate to low affinity for calcium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Aspartactin; calmitin; Calmitine; calsequestrin 1 (fast-twitch, skeletal muscle); Calsequestrin, skeletal muscle isoform; Calsequestrin-1; Laminin-binding protein
Gene Aliases: CASQ; CASQ1; CSQ; CSQ-1; CSQ1; PDIB1; sCSQ; VMCQA
UniProt ID: (Human) P31415, (Rat) P19633, (Mouse) O09165
Entrez Gene ID: (Sheep) 101118652, (Dog) 608401, (Human) 844, (Rat) 686019, (Mouse) 12372
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of store-operated calcium channel activity
regulation of store-operated calcium entry
endoplasmic reticulum organization
skeletal muscle tissue development
response to heat
response to organic substance
regulation of skeletal muscle contraction by regulation of release of sequestered calcium ion
response to denervation involved in regulation of muscle adaptation
ion transmembrane transport
sarcomere organization
protein polymerization
regulation of cardiac conduction
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
response to muscle inactivity
regulation of sequestering of calcium ion
regulation of muscle contraction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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