Invitrogen

Carbonic Anhydrase II Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

Questions & Answers

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Carbonic Anhydrase II Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Carbonic Anhydrase II was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-51598) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing predominantly cytoplasmic and plasma membrane localization. Panel e represents Hep G2 cells which has lower expression when compared to A-431 cells.. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.

Carbonic Anhydrase II Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of Carbonic Anhydrase II in human stomach and prostate tissues using Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-51598). Corresponding CA2 RNA-seq data are presented for the same tissues.

Carbonic Anhydrase II Antibody in Western Blot (WB) — Western blot was performed using Anti-Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-51598) and a 29 kDa band corresponding to Carbonic Anhydrase II was observed across all the cell lines and tissues tested except PC-3, Hep G2 and mouse skeletal muscle (Fig. a and Fig. b). Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), A-431 (Lane 2), HEK-293 (Lane 3), PC-3 (Lane 4), Hep G2 (Lane 5), Mouse Stomach (Lane 6), Mouse Kidney (Lane 7), Rat Kidney (Lane 8), Mouse Skeletal Muscle (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.2 µg/mL) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005).

Carbonic Anhydrase II Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of Carbonic Anhydrase II demonstrates antibody specificity. Immunohistochemical analysis of Carbonic Anhydrase II using anti-Carbonic Anhydrase II Polyclonal Antibody (Product # PA5-51598), shows significant staining of Carbonic Anhydrase II in human stomach and shows minimal or weak staining in human prostate tissues. The relative expression levels of Carbonic Anhydrase II within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-51598

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (IHC)	1:500-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human Carbonic Anhydrase II View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2639279

Product Specific Information

Immunogen sequence: YGKHNGPEHW HKDFPIAKGE RQSPVDIDTH TAKYDPSLKP LSVSYDQATS LRILNNGHAF NVEFDDSQDK AVLKGGPLDG TYRLIQFHFH WGSLDGQGSE HTVDKKKYAA ELHLVHWNTK YGDFGKAVQQ PDGLAVLG

Highest antigen sequence identity to the following orthologs: Mouse - 83%, Rat - 80%.

Target Information

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CA II; CA-II; CAC; Carbonate dehydratase II; Carbonic anhydrase 2; carbonic anhydrase B; Carbonic anhydrase C; Carbonic anhydrase II; carbonic dehydratase; Cyanamide hydratase CA2; epididymis luminal protein 76; epididymis secretory protein Li 282

Gene Aliases: AI131712; CA-II; CA2; CAC; CAII; Car-2; Car2; HEL-76; HEL-S-282; Ltw-5; Lvtw-5

UniProt ID: (Human) P00918, (Mouse) P00920, (Rat) P27139

Entrez Gene ID: (Human) 760, (Mouse) 12349, (Rat) 54231

Function(s)

morphogenesis of an epithelium one-carbon metabolic process regulation of pH carbon dioxide transport positive regulation of synaptic transmission, GABAergic positive regulation of cellular pH reduction angiotensin-activated signaling pathway regulation of anion transport positive regulation of osteoclast differentiation positive regulation of bone resorption secretion regulation of intracellular pH positive regulation of dipeptide transmembrane transport regulation of chloride transport kidney development response to pH response to zinc ion bicarbonate transport odontogenesis of dentin-containing tooth response to estrogen response to steroid hormone cellular response to fluid shear stress response to organic substance osteoclast differentiation regulation of cellular pH regulation of bone resorption

Process(es)

arylesterase activity carbonate dehydratase activity protein binding zinc ion binding lyase activity metal ion binding

Carbonic Anhydrase II Polyclonal Antibody

Product Details

PA5-51598

Western Blot (WB)

Immunohistochemistry (IHC)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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