Invitrogen

Carbonic Anhydrase II Polyclonal Antibody

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Carbonic Anhydrase II Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Carbonic Anhydrase II in paraffin-embedded human breast carcinoma tissue. Sample was incubated with polyclonal Carbonic Anhydrase II antibody (Product # PA5-86557) using a 1:100 dilution.

Carbonic Anhydrase II Antibody in Western Blot (WB) — Western blot analysis of Carbonic Anhydrase II in Lane 1: HCT116 whole cell lysate (40 µg), Lane 2: Kidney tissue lysate of mouse (40 µg), Lane 3: brain tissue lysate of rat (20 µg), Lane 4: HEK293T whole cell lysate (10 µg). Sample was incubated with Carbonic Anhydrase II polyclonal antibody (Product # PA5-86557) using a 1:500 dilution.

Product Details

PA5-86557

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide, corresponding to amino acids 201-250 of Human CA II. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2803329

Target Information

CA2 is one of several (at least 7) isozymes of carbonic anhydrase. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CA II; CA-II; CAC; Carbonate dehydratase II; Carbonic anhydrase 2; carbonic anhydrase B; Carbonic anhydrase C; Carbonic anhydrase II; carbonic dehydratase; epididymis luminal protein 76; epididymis secretory protein Li 282

Gene Aliases: AI131712; CA-II; CA2; CAC; CAII; Car-2; Car2; HEL-76; HEL-S-282; Ltw-5; Lvtw-5

UniProt ID: (Human) P00918, (Mouse) P00920, (Rat) P27139

Entrez Gene ID: (Human) 760, (Mouse) 12349, (Rat) 54231

Function(s)