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Caspase-1 Polyclonal Antibody
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FIGURE: 1 / 3
Caspase-1 Antibody (600-401-AC5) in ICC/IF
Immunofluorescence Microscopy of Caspase-1 antibody. Cell Type: HeLa cells. Fixation: 0.5% PFA. Antigen retrieval: not required. Primary antibody: Caspase-1 antibody at 20 µg/mL for 1 hr at RT. Secondary antibody: Fluorescein rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: Caspase-1 is cytoplasmic. Staining: Caspase-1 as red fluorescent signal.
Product Details
600-401-AC5
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-Caspase-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 15 amino acid synthetic peptide from near the internal region of human Caspase-1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. Members of this subfamily include caspase-1, -4, -5, and -12 and can activate proinflammatory cytokines such as IL-1-b and IL-18. Caspase-1 was initially identified as an IL-1-b-converting enzyme; later experiments revealed it to be a mammalian homolog of the C. elegans cell death gene ced-3 whose overexpression can induce apoptosis in fibroblasts.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CASP-1; caspase 1, apoptosis-related cysteine peptidase; Caspase-1; Caspase1; cysteine protease; ICE; IL-1 beta-converting enzyme; IL-1BC; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45; unnamed protein product
Gene Aliases: CASP1; ICE; IL1BC; IL1BCE; P45
UniProt ID: (Human) P29466
Entrez Gene ID: (Human) 834
endopeptidase activity
cysteine-type endopeptidase activity
protein binding
peptidase activity
cysteine-type peptidase activity
cysteine-type endopeptidase activator activity involved in apoptotic process
hydrolase activity
enzyme binding
kinase binding
cytokine binding
identical protein binding
CARD domain binding
negative regulation of transcription from RNA polymerase II promoter
plasma membrane repair
pattern recognition receptor signaling pathway
proteolysis
apoptotic process
inflammatory response
signal transduction
osmosensory signaling pathway
protein secretion
protein autoprocessing
protein catabolic process
response to lipopolysaccharide
positive regulation of interleukin-1 beta production
positive regulation of interleukin-18 production
interleukin-18-mediated signaling pathway
defense response to bacterium
regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
icosanoid biosynthetic process
ceramide biosynthetic process
regulation of inflammatory response
positive regulation of inflammatory response
protein maturation
defense response to virus
protein poly-ADP-ribosylation
pyroptosis
interleukin-1-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to mechanical stimulus
cellular response to interferon-gamma
cytokine precursor processing
signaling receptor ligand precursor processing
AIM2 inflammasome complex assembly
pyroptotic cell death
positive regulation of tumor necrosis factor-mediated signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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