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Immunohistochemical analysis of Caspase 1 in formalin-fixed, paraffin-embedded section of human reactive lymph node using a Caspase 1 polyclonal antibody (Product # PA1-41214), DAB chromogen and Hematoxylin counterstain. B is a higher magnification of A. Staining in macrophages is observed.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues Q(31) T R V L N K E E M E K V K R(45) of human Caspase-1.|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: antigen standard for CASP1 (transient overexpression lysate), Hela whole cell extract.
Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis (1). The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a tetramer to form the active enzyme. Caspase-1/ICE (IL-1b converting enzyme) is similar to the cell death gene CED-3 of Caenorhabditilis elegans and regulates multiple proinflammatory cytokines, including interleukin-1b and interferon-gamma-inducing factor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CASP1 nirs variant 1; caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase); ICE; IL-1 beta-converting enzyme; IL1B-convertase; IL1BC; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; P45
CASP1; ICE; IL1BC; IL1BCE; P45