Proteintech

Caspase 3 Polyclonal Antibody

Product Image Gallery

Caspase 3 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed NIH/3T3 cells using 19677-1-AP (Caspase 3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Caspase 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 19677-1-AP ( Caspase 3 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Caspase 3 Antibody in Western Blot (WB) — Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 19677-1-AP (Caspase 3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Caspase 3 Antibody in Immunoprecipitation (IP) — IP result of anti-Caspase 3 (IP:19677-1-AP, 4ug; Detection:19677-1-AP 1:300) with NIH/3T3 cells lysate 3440 ug.

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:50-1:500

Immunofluorescence (IF)

1:50-1:500

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:1000

View 316 publications 316 publications

Immunohistochemistry (IHC)

View 25 publications 25 publications

Miscellaneous PubMed (Misc)

View 14 publications 14 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Artificial Control, Hamster, Human, Mouse, Pig, Plant, Rat, Sheep, Zebrafish

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

This antibody can recognize p17, p19 and p32 of Caspase 3.

Target Information

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Apopain; Apopain precursor; CASP-3; Caspase 3 apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis related cysteine protease; Caspase 3, apoptosis related cysteine protease (ICE-like cysteine protease); caspase 3, apoptosis-related cysteine peptidase; caspase 3, apoptosis-related cysteine protease; Caspase-3; Caspase-3 subunit p12; Caspase-3 subunit p17; Caspase3; CPP-32; CPP32beta; Cysteine protease CPP32; ICE3; IRP; LICE; PARP cleavage protease; procaspase3; Protein Yama; SCA-1; SREBP cleavage activity 1; yama protein

Gene Aliases: A830040C14Rik; AC-3; Apopain; CASP-3; CASP3; Caspase-3; CC3; CPP-32; CPP32; CPP32B; Lice; mldy; SCA-1; Yama

UniProt ID: (Human) P42574, (Mouse) P70677, (Rat) P55213

Entrez Gene ID: (Human) 836, (Mouse) 12367, (Rat) 25402

Molecular Function: cysteine protease enzyme modulator hydrolase protease protease inhibitor